Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Milrad de Forchetti, S.R.; Cazzulo, J.J.
    Some properties of the pyruvate carboxylase from Pseudomonas fluorescens (1976), J. Gen. Microbiol., 93, 75-81.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.4.1.1 acetyl-CoA no effect Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.1 ADP strong Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.1 0.08
-
 ATP pyruvate, second values 0.21 mM Pseudomonas fluorescens
6.4.1.1 0.21
-
 pyruvate second value 0.08 Pseudomonas fluorescens
6.4.1.1 3.1
-
 HCO3-
-
 Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.1 K+ monovalent cations activate, NH4+ or K+ Pseudomonas fluorescens
6.4.1.1 Mg2+ activates Pseudomonas fluorescens
6.4.1.1 NH4+ monovalent cations activate, NH4+ or K+ Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.1 300000
-
 gel filtration Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.1 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.1
-
 Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.1 9.34
-
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.1 ATP + pyruvate + HCO3-
-
 Pseudomonas fluorescens ADP + phosphate + oxaloacetate
-
 ?
6.4.1.1 ATP + pyruvate + HCO3- ATP in form of MgATP2- Pseudomonas fluorescens ADP + phosphate + oxaloacetate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.4.1.1 46
-
 10 min, 50% loss of activity Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.1 7.1
-
 in presence of MnATP2- Pseudomonas fluorescens
6.4.1.1 9
-
 in presence of MgATP2- Pseudomonas fluorescens