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Literature summary extracted from

  • Norris, R.D.; Fowden, L.
    Substrate discrimination by prolyl-tRNA synthetase from various higher plants (1972), Phytochemistry, 11, 2921-2935.
No PubMed abstract available

General Stability

EC Number General Stability Organism
6.1.1.15 L-azetidine-2-carboxylic acid, cis(exo)-3,4-methano-L-proline, 3,4-dehydro-DL-proline, L-thiazolidine-4-carboxylic acid, N-methylglycine, N-methyl-L-alanine or trans-3-hydroxy-L-proline protect against inactivation at 70°C and 40°C Phaseolus vulgaris
6.1.1.15 L-azetidine-2-carboxylic acid, cis(exo)-3,4-methano-L-proline, 3,4-dehydro-DL-proline, L-thiazolidine-4-carboxylic acid, N-methylglycine, N-methyl-L-alanine or trans-3-hydroxy-L-proline protect against inactivation at 70°C and 40°C Delonix regia
6.1.1.15 Pro or ATP markedly stabilizes against heat inactivation Phaseolus vulgaris
6.1.1.15 Pro or ATP markedly stabilizes against heat inactivation Delonix regia
6.1.1.15 Pro or ATP markedly stabilizes against heat inactivation Parkinsonia aculeata
6.1.1.15 several analogues of Pro protect against thermal inactivation Phaseolus vulgaris
6.1.1.15 several analogues of Pro protect against thermal inactivation Delonix regia

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.15 2-Pyrrolidinone
-
 Phaseolus vulgaris
6.1.1.15 3,4-Dehydroproline
-
 Delonix regia
6.1.1.15 3,4-Dehydroproline
-
 Phaseolus vulgaris
6.1.1.15 3-pyrroline
-
 Delonix regia
6.1.1.15 3-pyrroline
-
 Phaseolus vulgaris
6.1.1.15 4-Methylene-DL-proline weak Delonix regia
6.1.1.15 cis(exo)-3,4-Methano-L-proline
-
 Delonix regia
6.1.1.15 cis(exo)-3,4-Methano-L-proline
-
 Phaseolus vulgaris
6.1.1.15 cis-3-hydroxy-L-proline weak Delonix regia
6.1.1.15 cis-3-hydroxy-L-proline weak Phaseolus vulgaris
6.1.1.15 CsCl concentration required to inhibit ATP-diphosphate exchange by 50%: above 2 M Beta vulgaris
6.1.1.15 CsCl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.5 M Delonix regia
6.1.1.15 CsCl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.51 M Parkinsonia aculeata
6.1.1.15 CsCl concentration required to inhibit ATP-diphosphate exchange by 50%: 1.7 M Phaseolus vulgaris
6.1.1.15 DL-Proline amide
-
 Phaseolus vulgaris
6.1.1.15 KCl concentration required to inhibit ATP-diphosphate exchange by 50%: above 2 M Beta vulgaris
6.1.1.15 KCl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.37 M Delonix regia
6.1.1.15 KCl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.4 M Parkinsonia aculeata
6.1.1.15 KCl concentration required to inhibit ATP-diphosphate exchange by 50%: 2.1 M Phaseolus vulgaris
6.1.1.15 L-azetidine-2-carboxylic acid
-
 Delonix regia
6.1.1.15 L-azetidine-2-carboxylic acid
-
 Phaseolus vulgaris
6.1.1.15 L-thiazolidine-4-carboxylic acid
-
 Delonix regia
6.1.1.15 L-thiazolidine-4-carboxylic acid
-
 Phaseolus vulgaris
6.1.1.15 LiCl concentration required to inhibit ATP-diphosphate exchange by 50%: 1.2 M Beta vulgaris
6.1.1.15 LiCl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.25 M Delonix regia
6.1.1.15 LiCl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.23 M Parkinsonia aculeata
6.1.1.15 LiCl concentration required to inhibit ATP-diphosphate exchange by 50%: 1.2 M Phaseolus vulgaris
6.1.1.15 N-ethylglycine weak Delonix regia
6.1.1.15 N-Methyl-L-alanine weak Phaseolus vulgaris
6.1.1.15 N-methylglycine weak Delonix regia
6.1.1.15 N-methylglycine
-
 Phaseolus vulgaris
6.1.1.15 NaCl concentration required to inhibit ATP-diphosphate exchange by 50%: 1.5 M Beta vulgaris
6.1.1.15 NaCl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.3 M Delonix regia
6.1.1.15 NaCl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.27 M Parkinsonia aculeata
6.1.1.15 NaCl concentration required to inhibit ATP-diphosphate exchange by 50%: 1.43 M Phaseolus vulgaris
6.1.1.15 NH4Cl concentration required to inhibit ATP-diphosphate exchange by 50%: 1.0 M Beta vulgaris
6.1.1.15 NH4Cl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.35 M Delonix regia
6.1.1.15 NH4Cl concentration required to inhibit ATP-diphosphate exchange by 50%: 0.38 M Parkinsonia aculeata
6.1.1.15 NH4Cl concentration required to inhibit ATP-diphosphate exchange by 50%: 1.1 M Phaseolus vulgaris
6.1.1.15 Pyrrole
-
 Delonix regia
6.1.1.15 Pyrrole
-
 Phaseolus vulgaris
6.1.1.15 Pyrrolidine
-
 Delonix regia
6.1.1.15 Pyrrolidine
-
 Phaseolus vulgaris
6.1.1.15 tetrahydrofuran
-
 Delonix regia
6.1.1.15 tetrahydrofuran
-
 Phaseolus vulgaris
6.1.1.15 Tetrahydrothiophen
-
 Delonix regia
6.1.1.15 Tetrahydrothiophen
-
 Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.15 0.137
-
 L-Pro ATP-diphosphate exchange Phaseolus vulgaris
6.1.1.15 0.182
-
 L-Pro ATP-diphosphate exchange Delonix regia
6.1.1.15 0.28
-
 3,4-dehydro-DL-proline ATP-diphosphate exchange Phaseolus vulgaris
6.1.1.15 0.29
-
 L-Pro ATP-diphosphate exchange Ranunculus bulbosus
6.1.1.15 0.364
-
 3,4-dehydro-DL-proline ATP-diphosphate exchange Ranunculus bulbosus
6.1.1.15 0.43
-
 L-Pro ATP-diphosphate exchange Parkinsonia aculeata
6.1.1.15 0.45
-
 L-Pro ATP-diphosphate exchange Beta vulgaris
6.1.1.15 0.45
-
 L-Pro ATP-diphosphate exchange Convallaria majalis
6.1.1.15 0.5
-
 3,4-dehydro-DL-proline ATP-diphosphate exchange Beta vulgaris
6.1.1.15 0.625
-
 L-Pro ATP-diphosphate exchange Hemerocallis fulva
6.1.1.15 0.74
-
 3,4-dehydro-DL-proline ATP-diphosphate exchange Hemerocallis fulva
6.1.1.15 0.78
-
 3,4-dehydro-DL-proline ATP-diphosphate exchange Delonix regia
6.1.1.15 1.4
-
 3-thiaproline 3,4-dehydro-DL-proline, , ATP-diphosphate exchange Convallaria majalis
6.1.1.15 1.43
-
 L-azetidine-2-carboxylic acid ATP-diphosphate exchange Phaseolus vulgaris
6.1.1.15 2
-
 L-azetidine-2-carboxylic acid ATP-diphosphate exchange Ranunculus bulbosus
6.1.1.15 2 3 cis(exo)-3,4-Methano-L-proline ATP-diphosphate exchange Convallaria majalis
6.1.1.15 2.2
-
 3,4-dehydro-DL-proline ATP-diphosphate exchange Beta vulgaris
6.1.1.15 2.2
-
 3,4-dehydro-DL-proline ATP-diphosphate exchange, L-azetidine-2-carboxylic acid Parkinsonia aculeata
6.1.1.15 4.6
-
 cis(exo)-3,4-Methano-L-proline ATP-diphosphate exchange Delonix regia
6.1.1.15 5.3
-
 L-azetidine-2-carboxylic acid ATP-diphosphate exchange Hemerocallis fulva
6.1.1.15 7.1
-
 cis(exo)-3,4-Methano-L-proline ATP-diphosphate exchange Parkinsonia aculeata
6.1.1.15 20 50 L-thiazolidine-4-carboxylic acid ATP-diphosphate exchange Delonix regia
6.1.1.15 20
-
 thiazolidine-4-carboxylic acid ATP-diphosphate exchange Phaseolus vulgaris
6.1.1.15 45
-
 N-methylglycine ATP-diphosphate exchange Parkinsonia aculeata
6.1.1.15 67
-
 N-methylglycine ATP-diphosphate exchange Phaseolus vulgaris
6.1.1.15 68
-
 N-Methyl-L-alanine ATP-diphosphate exchange Phaseolus vulgaris
6.1.1.15 100
-
 N-methylglycine ATP-diphosphate exchange Hemerocallis fulva
6.1.1.15 140
-
 N-methylglycine ATP-diphosphate exchange Ranunculus bulbosus
6.1.1.15 300
-
 N-methylglycine approximate value, , ATP-diphosphate exchange Delonix regia

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.15 Beta vulgaris
-
-
-
6.1.1.15 Convallaria majalis
-
-
-
6.1.1.15 Delonix regia
-
-
-
6.1.1.15 Hemerocallis fulva
-
-
-
6.1.1.15 Parkinsonia aculeata
-
-
-
6.1.1.15 Phaseolus vulgaris
-
-
-
6.1.1.15 Polygonatum multiflorum
-
-
-
6.1.1.15 Ranunculus bulbosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.15
-
 Phaseolus vulgaris
6.1.1.15
-
 Delonix regia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.15 0.101
-
-
 Delonix regia
6.1.1.15 1.655
-
-
 Delonix regia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.15 ATP + 3,4-dehydro-DL-proline + tRNAPro
-
 Phaseolus vulgaris ?
-
 ?
6.1.1.15 ATP + 3,4-dehydro-DL-proline + tRNAPro
-
 Delonix regia ?
-
 ?
6.1.1.15 ATP + 3,4-dehydro-DL-proline + tRNAPro
-
 Hemerocallis fulva ?
-
 ?
6.1.1.15 ATP + 3,4-dehydro-DL-proline + tRNAPro
-
 Ranunculus bulbosus ?
-
 ?
6.1.1.15 ATP + 3,4-dehydro-DL-proline + tRNAPro
-
 Beta vulgaris ?
-
 ?
6.1.1.15 ATP + 3,4-dehydro-DL-proline + tRNAPro
-
 Parkinsonia aculeata ?
-
 ?
6.1.1.15 ATP + 3-thiaproline + tRNAPro
-
 Convallaria majalis ?
-
 ?
6.1.1.15 ATP + cis(exo)-3,4-methano-L-proline + tRNAPro
-
 Delonix regia ?
-
 ?
6.1.1.15 ATP + cis(exo)-3,4-methano-L-proline + tRNAPro
-
 Parkinsonia aculeata ?
-
 ?
6.1.1.15 ATP + cis(exo)-3,4-methano-L-proline + tRNAPro
-
 Convallaria majalis ?
-
 ?
6.1.1.15 ATP + L-azetidine-2-carboxylic acid + tRNAPro
-
 Phaseolus vulgaris ?
-
 ?
6.1.1.15 ATP + L-azetidine-2-carboxylic acid + tRNAPro
-
 Hemerocallis fulva ?
-
 ?
6.1.1.15 ATP + L-azetidine-2-carboxylic acid + tRNAPro
-
 Ranunculus bulbosus ?
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro
-
 Polygonatum multiflorum AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro N-methylglycine can replace proline in ATP-diphosphate exchange Phaseolus vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro N-methylglycine can replace proline in ATP-diphosphate exchange Delonix regia AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro N-methylglycine can replace proline in ATP-diphosphate exchange Hemerocallis fulva AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro N-methylglycine can replace proline in ATP-diphosphate exchange Ranunculus bulbosus AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro N-methylglycine can replace proline in ATP-diphosphate exchange Beta vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro N-methylglycine can replace proline in ATP-diphosphate exchange Parkinsonia aculeata AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro N-methylglycine can replace proline in ATP-diphosphate exchange Convallaria majalis AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro L-azetidine-2-carboxylic acid can replace proline in ATP-diphosphate exchange (not) Phaseolus vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro L-azetidine-2-carboxylic acid can replace proline in ATP-diphosphate exchange (not) Hemerocallis fulva AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro L-azetidine-2-carboxylic acid can replace proline in ATP-diphosphate exchange (not) Ranunculus bulbosus AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro L-azetidine-2-carboxylic acid can replace proline in ATP-diphosphate exchange (not) Beta vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro cis(exo)-3,4-methano-L-proline can replace proline on ATP-diphosphate exchange Delonix regia AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro cis(exo)-3,4-methano-L-proline can replace proline on ATP-diphosphate exchange Parkinsonia aculeata AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro cis(exo)-3,4-methano-L-proline can replace proline on ATP-diphosphate exchange Convallaria majalis AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Phaseolus vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Delonix regia AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Hemerocallis fulva AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Ranunculus bulbosus AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Beta vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Parkinsonia aculeata AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Convallaria majalis AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro L-thiazolidine-4-carboxylic acid can replace proline in ATP-diphosphate exchange Phaseolus vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro L-thiazolidine-4-carboxylic acid can replace proline in ATP-diphosphate exchange Delonix regia AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro L-thiazolidine-4-carboxylic acid can replace proline in ATP-diphosphate exchange Ranunculus bulbosus AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro L-thiazolidine-4-carboxylic acid can replace proline in ATP-diphosphate exchange Beta vulgaris AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro L-thiazolidine-4-carboxylic acid can replace proline in ATP-diphosphate exchange Parkinsonia aculeata AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-thiazolidine-4-carboxylic acid + tRNAPro
-
 Delonix regia ?
-
 ?
6.1.1.15 ATP + N-methyl-L-alanine + tRNAPro
-
 Phaseolus vulgaris ?
-
 ?
6.1.1.15 ATP + N-methylglycine + tRNAPro
-
 Phaseolus vulgaris ?
-
 ?
6.1.1.15 ATP + N-methylglycine + tRNAPro
-
 Delonix regia ?
-
 ?
6.1.1.15 ATP + N-methylglycine + tRNAPro
-
 Hemerocallis fulva ?
-
 ?
6.1.1.15 ATP + N-methylglycine + tRNAPro
-
 Ranunculus bulbosus ?
-
 ?
6.1.1.15 ATP + N-methylglycine + tRNAPro
-
 Parkinsonia aculeata ?
-
 ?
6.1.1.15 ATP + thiazolidine-4-carboxylic acid + tRNAPro
-
 Phaseolus vulgaris ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.15 25
-
 50% loss of ATP-diphosphate exchange activity after 5 min, without stabilizing reagent Delonix regia
6.1.1.15 25
-
 50% loss of ATP-diphosphate exchange activity after 5 min, without stabilizing reagent Parkinsonia aculeata
6.1.1.15 51
-
 50% loss of ATP-diphosphate exchange activity after 5 min, without stabilizing reagent Ranunculus bulbosus
6.1.1.15 55
-
 50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.002 mM ATP Parkinsonia aculeata
6.1.1.15 56
-
 50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.002 mM ATP Delonix regia
6.1.1.15 58
-
 50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.02 mM Pro Parkinsonia aculeata
6.1.1.15 59
-
 50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.02 mM Pro Delonix regia
6.1.1.15 65
-
 50% loss of ATP-diphosphate exchange activity after 5 min, without stabilizing reagent Phaseolus vulgaris
6.1.1.15 67
-
 50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.002 mM ATP + 0.02 mM Pro Delonix regia
6.1.1.15 67
-
 50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.002 mM ATP + 0.02 mM Pro Parkinsonia aculeata
6.1.1.15 73
-
 50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.002 mM ATP Phaseolus vulgaris
6.1.1.15 78
-
 50% loss of ATP-diphosphate exchange activity after 5 min, in presence of 0.002 mM ATP + 0.02 mM Pro Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.15 7.5 7.8 ATP-diphosphate exchange Phaseolus vulgaris
6.1.1.15 7.5 7.8 ATP-diphosphate exchange Ranunculus bulbosus
6.1.1.15 7.6 7.9 ATP-diphosphate exchange Hemerocallis fulva
6.1.1.15 7.9 8.3 ATP-diphosphate exchange Beta vulgaris
6.1.1.15 8.4 8.7 ATP-diphosphate exchange Convallaria majalis
6.1.1.15 8.4 8.8 ATP-diphosphate exchange Polygonatum multiflorum
6.1.1.15 8.8 9.2 ATP-diphosphate exchange Parkinsonia aculeata
6.1.1.15 9 9.4 ATP-diphosphate exchange Delonix regia