EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.3.5.5 | 5-phospho-alpha-D-ribosyl 1-diphosphate | stimulates | Rattus norvegicus |
EC Number | General Stability | Organism |
---|---|---|
6.3.5.5 | enzyme is most stable in the presence of 30% dimethyl sulfoxide, 5% glycerol, 1 mM dithiothreitol. MgATP2-, MgUTP2-, and 5-phospho-alpha-D-ribosyl 1-diphosphate effectively stabilize | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.5.5 | 4-hydroxymercuribenzoate | - |
Rattus norvegicus | |
6.3.5.5 | ADP | competitive with MgATP2- | Rattus norvegicus | |
6.3.5.5 | Ala | inhibits glutamine hydrolysis and Gln-dependent carbamoyl phosphate synthesis | Rattus norvegicus | |
6.3.5.5 | CTP | - |
Rattus norvegicus | |
6.3.5.5 | dUDP | - |
Rattus norvegicus | |
6.3.5.5 | dUTP | - |
Rattus norvegicus | |
6.3.5.5 | Gly | - |
Rattus norvegicus | |
6.3.5.5 | Mersalyl | - |
Rattus norvegicus | |
6.3.5.5 | polyamines | e.g. spermine, spermidine or putrescine | Rattus norvegicus | |
6.3.5.5 | putrescine | - |
Rattus norvegicus | |
6.3.5.5 | spermidine | - |
Rattus norvegicus | |
6.3.5.5 | spermine | - |
Rattus norvegicus | |
6.3.5.5 | UDP | - |
Rattus norvegicus | |
6.3.5.5 | UDPglucose | - |
Rattus norvegicus | |
6.3.5.5 | UTP | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.5.5 | 0.021 | - |
L-Gln | in 10% glycerol, pH 7 | Rattus norvegicus | |
6.3.5.5 | 1 | - |
HCO3- | in 30% dimethyl sulfoxide | Rattus norvegicus | |
6.3.5.5 | 1.7 | - |
ATP | MgATP2-, in 10% glycerol, pH 7 | Rattus norvegicus | |
6.3.5.5 | 10 | - |
HCO3- | in 10% glycerol, pH 7 | Rattus norvegicus | |
6.3.5.5 | 15 | - |
NH4+ | in 10% glycerol, pH 7 | Rattus norvegicus | |
6.3.5.5 | 20 | - |
HCO3- | in absence of cryoprotectants | Rattus norvegicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.5.5 | K+ | required for maximal activity | Rattus norvegicus | |
6.3.5.5 | K+ | Km: 18 mM | Rattus norvegicus | |
6.3.5.5 | Mg2+ | optimal concentration: 5 mM | Rattus norvegicus | |
6.3.5.5 | Mg2+ | free Mg2+ is required in addition to MgATP2- | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.2.3 | 210000 | - |
4 * 210000, SDS-PAGE | Rattus norvegicus |
3.5.2.3 | 870000 | - |
ultracentrifugation | Rattus norvegicus |
6.3.5.5 | additional information | - |
the multienzyme complex of carbamoyl-phosphate synthase(glutamine), aspartate carbamoyltransferase and dihydroorotase has a MW of 870000 as determined by sedimentation equilibrium studies and 210000 by SDS-PAGE | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.3.2 | carbamoylphosphate + L-aspartate | Rattus norvegicus | - |
phosphate + N-carbamoyl-L-aspartate | - |
? | |
2.1.3.2 | carbamoylphosphate + L-aspartate | Mesocricetus auratus | - |
phosphate + N-carbamoyl-L-aspartate | - |
? | |
3.5.2.3 | N-carbamoyl-L-aspartate | Rattus norvegicus | - |
L-dihydroorotate + H2O | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.3.2 | Mesocricetus auratus | - |
- |
- |
2.1.3.2 | Rattus norvegicus | - |
- |
- |
3.5.2.3 | Rattus norvegicus | - |
- |
- |
6.3.5.5 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.3.2 | multienzyme complex | Rattus norvegicus |
2.1.3.2 | multienzyme complex | Mesocricetus auratus |
3.5.2.3 | - |
Rattus norvegicus |
6.3.5.5 | multienzyme complex of carbamoyl-phosphate synthase(glutamine), aspartate carbamoyltransferase and dihydroorotase | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.3.2 | hepatoma ascites cell | - |
Rattus norvegicus | - |
2.1.3.2 | liver | - |
Rattus norvegicus | - |
2.1.3.2 | liver | - |
Mesocricetus auratus | - |
3.5.2.3 | hepatoma ascites cell line | - |
Rattus norvegicus | - |
3.5.2.3 | liver | - |
Rattus norvegicus | - |
6.3.5.5 | hepatoma ascites cell | AH13 | Rattus norvegicus | - |
6.3.5.5 | liver | - |
Rattus norvegicus | - |
EC Number | Storage Stability | Organism |
---|---|---|
3.5.2.3 | -80°C | Rattus norvegicus |
6.3.5.5 | -80°C, 30% dimethyl sulfoxide, 5% glycerol, 1 mM dithiothreitol, stable for 6 months | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.3.2 | carbamoyl phosphate + L-aspartate | - |
Rattus norvegicus | phosphate + N-carbamoyl-L-aspartate | - |
? | |
2.1.3.2 | carbamoyl phosphate + L-aspartate | - |
Mesocricetus auratus | phosphate + N-carbamoyl-L-aspartate | - |
? | |
2.1.3.2 | carbamoylphosphate + L-aspartate | - |
Rattus norvegicus | phosphate + N-carbamoyl-L-aspartate | - |
? | |
2.1.3.2 | carbamoylphosphate + L-aspartate | - |
Mesocricetus auratus | phosphate + N-carbamoyl-L-aspartate | - |
? | |
3.5.2.3 | L-carbamoylaspartate | - |
Rattus norvegicus | L-dihydroorotate + H2O | - |
r | |
3.5.2.3 | N-carbamoyl-L-aspartate | - |
Rattus norvegicus | L-dihydroorotate + H2O | - |
r | |
6.3.5.5 | 2 ATP + L-Gln + HCO3- | - |
Rattus norvegicus | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
6.3.5.5 | 2 ATP + NH4+ + HCO3- | - |
Rattus norvegicus | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
6.3.5.5 | ATP + L-glutamine + hydrogencarbonate + H2O | ATP in form of MgATP2- | Rattus norvegicus | ADP + phosphate + L-glutamate + carbamoyl phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.3.2 | More | - |
Mesocricetus auratus |
3.5.2.3 | multimer | - |
Rattus norvegicus |
3.5.2.3 | tetramer | 4 * 210000, SDS-PAGE | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.5.5 | 6.8 | 7.2 | Gln-dependent activity | Rattus norvegicus |
6.3.5.5 | 7.8 | - |
NH4+-dependent activity | Rattus norvegicus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
6.3.5.5 | 7 | - |
half-life: 20 min | Rattus norvegicus |