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Literature summary extracted from

  • Mori, M.; Tatibana, M.
    A multienzyme complex of carbamoyl-phosphate synthase (glutamine):aspartate carbamoyltransferase:dihydroorotase (rat ascites hepatoma cells and rat liver) (1978), Methods Enzymol., 51, 111-121.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.5.5 5-phospho-alpha-D-ribosyl 1-diphosphate stimulates Rattus norvegicus

General Stability

EC Number General Stability Organism
6.3.5.5 enzyme is most stable in the presence of 30% dimethyl sulfoxide, 5% glycerol, 1 mM dithiothreitol. MgATP2-, MgUTP2-, and 5-phospho-alpha-D-ribosyl 1-diphosphate effectively stabilize Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.5 4-hydroxymercuribenzoate
-
Rattus norvegicus
6.3.5.5 ADP competitive with MgATP2- Rattus norvegicus
6.3.5.5 Ala inhibits glutamine hydrolysis and Gln-dependent carbamoyl phosphate synthesis Rattus norvegicus
6.3.5.5 CTP
-
Rattus norvegicus
6.3.5.5 dUDP
-
Rattus norvegicus
6.3.5.5 dUTP
-
Rattus norvegicus
6.3.5.5 Gly
-
Rattus norvegicus
6.3.5.5 Mersalyl
-
Rattus norvegicus
6.3.5.5 polyamines e.g. spermine, spermidine or putrescine Rattus norvegicus
6.3.5.5 putrescine
-
Rattus norvegicus
6.3.5.5 spermidine
-
Rattus norvegicus
6.3.5.5 spermine
-
Rattus norvegicus
6.3.5.5 UDP
-
Rattus norvegicus
6.3.5.5 UDPglucose
-
Rattus norvegicus
6.3.5.5 UTP
-
Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.5 0.021
-
L-Gln in 10% glycerol, pH 7 Rattus norvegicus
6.3.5.5 1
-
HCO3- in 30% dimethyl sulfoxide Rattus norvegicus
6.3.5.5 1.7
-
ATP MgATP2-, in 10% glycerol, pH 7 Rattus norvegicus
6.3.5.5 10
-
HCO3- in 10% glycerol, pH 7 Rattus norvegicus
6.3.5.5 15
-
NH4+ in 10% glycerol, pH 7 Rattus norvegicus
6.3.5.5 20
-
HCO3- in absence of cryoprotectants Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.5 K+ required for maximal activity Rattus norvegicus
6.3.5.5 K+ Km: 18 mM Rattus norvegicus
6.3.5.5 Mg2+ optimal concentration: 5 mM Rattus norvegicus
6.3.5.5 Mg2+ free Mg2+ is required in addition to MgATP2- Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.3 210000
-
4 * 210000, SDS-PAGE Rattus norvegicus
3.5.2.3 870000
-
ultracentrifugation Rattus norvegicus
6.3.5.5 additional information
-
the multienzyme complex of carbamoyl-phosphate synthase(glutamine), aspartate carbamoyltransferase and dihydroorotase has a MW of 870000 as determined by sedimentation equilibrium studies and 210000 by SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.2 carbamoylphosphate + L-aspartate Rattus norvegicus
-
phosphate + N-carbamoyl-L-aspartate
-
?
2.1.3.2 carbamoylphosphate + L-aspartate Mesocricetus auratus
-
phosphate + N-carbamoyl-L-aspartate
-
?
3.5.2.3 N-carbamoyl-L-aspartate Rattus norvegicus
-
L-dihydroorotate + H2O
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Mesocricetus auratus
-
-
-
2.1.3.2 Rattus norvegicus
-
-
-
3.5.2.3 Rattus norvegicus
-
-
-
6.3.5.5 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.2 multienzyme complex Rattus norvegicus
2.1.3.2 multienzyme complex Mesocricetus auratus
3.5.2.3
-
Rattus norvegicus
6.3.5.5 multienzyme complex of carbamoyl-phosphate synthase(glutamine), aspartate carbamoyltransferase and dihydroorotase Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.3.2 hepatoma ascites cell
-
Rattus norvegicus
-
2.1.3.2 liver
-
Rattus norvegicus
-
2.1.3.2 liver
-
Mesocricetus auratus
-
3.5.2.3 hepatoma ascites cell line
-
Rattus norvegicus
-
3.5.2.3 liver
-
Rattus norvegicus
-
6.3.5.5 hepatoma ascites cell AH13 Rattus norvegicus
-
6.3.5.5 liver
-
Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
3.5.2.3 -80°C Rattus norvegicus
6.3.5.5 -80°C, 30% dimethyl sulfoxide, 5% glycerol, 1 mM dithiothreitol, stable for 6 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate
-
Rattus norvegicus phosphate + N-carbamoyl-L-aspartate
-
?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
Mesocricetus auratus phosphate + N-carbamoyl-L-aspartate
-
?
2.1.3.2 carbamoylphosphate + L-aspartate
-
Rattus norvegicus phosphate + N-carbamoyl-L-aspartate
-
?
2.1.3.2 carbamoylphosphate + L-aspartate
-
Mesocricetus auratus phosphate + N-carbamoyl-L-aspartate
-
?
3.5.2.3 L-carbamoylaspartate
-
Rattus norvegicus L-dihydroorotate + H2O
-
r
3.5.2.3 N-carbamoyl-L-aspartate
-
Rattus norvegicus L-dihydroorotate + H2O
-
r
6.3.5.5 2 ATP + L-Gln + HCO3-
-
Rattus norvegicus 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
6.3.5.5 2 ATP + NH4+ + HCO3-
-
Rattus norvegicus 2 ADP + phosphate + carbamoyl phosphate
-
?
6.3.5.5 ATP + L-glutamine + hydrogencarbonate + H2O ATP in form of MgATP2- Rattus norvegicus ADP + phosphate + L-glutamate + carbamoyl phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.1.3.2 More
-
Mesocricetus auratus
3.5.2.3 multimer
-
Rattus norvegicus
3.5.2.3 tetramer 4 * 210000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.5 6.8 7.2 Gln-dependent activity Rattus norvegicus
6.3.5.5 7.8
-
NH4+-dependent activity Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.5.5 7
-
half-life: 20 min Rattus norvegicus