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Literature summary extracted from

  • Arner, R.J.; Prabhu, K.S.; Thompson, J.T.; Hildenbrandt, G.R.; Liken, A.D.; Reddy, C.C.
    myo-Inositol oxygenase: molecular cloning and expression of a unique enzyme that oxidizes myo-inositol and D-chiro-inositol (2001), Biochem. J., 360, 313-320.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.99.1 cysteine 1 mM Fe2+ plus 2 mM cysteine required Sus scrofa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.99.1 open reading frame of 849 base pairs Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.99.1 5.9
-
 myo-inositol with Fe(2+) and L-cysteine as activators Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.99.1 Fe2+ 1 mM Fe2+ plus 2mM cysteine required Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.99.1 32663
-
 electrospray MS determination, gel filtration, SDS-PAGE, sedimentation equilibirum, enzyme does not undergo oligomerization in the presence of myo-inositol Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.99.1 myo-inositol + O2 Sus scrofa major role in pathogenesis of diabetis d-glucuronate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.99.1 Sus scrofa Q8WN98 SwissProt Accession Code:Q8WN98
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.99.1
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.99.1 kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.99.1 D-chiro-inositol + O2 much less active with D-chiro-inositol than with myo-inositol Sus scrofa D-glucuronate
-
 ?
1.13.99.1 myo-inositol + O2 highly specific for myo-inositol Sus scrofa D-glucuronate + H2O
-
 ?
1.13.99.1 myo-inositol + O2 major role in pathogenesis of diabetis Sus scrofa d-glucuronate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.99.1 monomer 1 * 32663, electrospray MS determination, gel filtration, SDS-PAGE, sedimentation equilibirum, enzyme does not undergo oligomerization in the presence of myo-inositol Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.99.1 0.183
-
 myo-inositol with Fe(2+) and L-cysteine as activators Sus scrofa