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Literature summary extracted from

  • Powell, G.K.; Dekker, E.E.
    Purification and properties of a 4-methylene-L-glutamine amidohydrolase from peanut leaves (1983), J. Biol. Chem., 258, 8677-8683.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.67 additional information activation studies Arachis hypogaea
3.5.1.67 additional information no activation by thiols or sulfhydryl-reacting reagents Arachis hypogaea
3.5.1.67 SDS activation Arachis hypogaea
3.5.1.67 SDS 0.001-1%, 2-3fold stimulation, 4-methylene-L-glutamine or glutamine as substrate Arachis hypogaea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.67 arsenate no inhibition Arachis hypogaea
3.5.1.67 BO33- strong inhibition, 1 mM: 50% inhibition Arachis hypogaea
3.5.1.67 Cu2+ no inhibition Arachis hypogaea
3.5.1.67 additional information inhibition studies; no inhibition by thiols, sulfhydryl-reacting reagents, glutaramic acid, glutarimide, acrylamide, methacrylamide, succinamic acid, acetamide, N-benzoyl-4-methylene-L-glutamine, isoglutamine, 4-methyleneisoglutamine, N-ethylmaleimide, p-mercuribenzoate, 5,5'-dithiobis(2-nitrobenzoic acid), dithiothreitol, chelating agents, EDTA, EGTA, 8-hydroxyquinoline, 8-hydroxyquinoline sulfonic acid, citrate, 2,2'-dipyridyl, 1 mM of following metal ions: Ca2+, Cd2+, Cu2+, Fe2+, Fe3+, Hg2+, Mg2+, Mn2+, Ni2+, Zn2+, 0.1-10 mM: CO32-, SO32-, SO42-, NO3-, AsO43- Arachis hypogaea
3.5.1.67 Urea slight inhibition, 10 mM: 10% inhibition Arachis hypogaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.67 additional information
-
 additional information kinetic data Arachis hypogaea
3.5.1.67 3
-
 4-methylene-L-glutamine
-
 Arachis hypogaea
3.5.1.67 19.5
-
 L-glutamine
-
 Arachis hypogaea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.67 cytosol present in soluble fraction Arachis hypogaea 5829
-
3.5.1.67 additional information subcellular localization Arachis hypogaea
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.67 additional information metal ion not required Arachis hypogaea
3.5.1.67 phosphate no activation observed Arachis hypogaea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.67 42000
-
 gel filtration Arachis hypogaea
3.5.1.67 48600
-
 1 * 48600, SDS-PAGE Arachis hypogaea
3.5.1.67 73000
-
 sucrose density gradient centrifugation, possibly the result of aggregation in the presence of high sucrose concentrations Arachis hypogaea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.67 4-methylene-L-glutamine + H2O Arachis hypogaea highly specific ?
-
 ?
3.5.1.67 4-methylene-L-glutamine + H2O Arachis hypogaea implicated in the process of N-translocation ?
-
 ?
3.5.1.67 4-methylene-L-glutamine + H2O Arachis hypogaea initial enzyme for catalyzing 4-methylene-L-glutamine degradation ?
-
 ?
3.5.1.67 4-methylene-L-glutamine + H2O Arachis hypogaea implicated in the transport of nitrogen to the developing shoots and leaves of germinating peanuts, involved in releasing nitrogen to the plants ?
-
 ?
3.5.1.67 additional information Arachis hypogaea implicated in the process of N-translocation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.67 Arachis hypogaea
-
 multiple forms: AH-1, AH-2, AH-3
-
3.5.1.67 Arachis hypogaea
-
 multiple electrophoretically distinct forms
-
3.5.1.67 Arachis hypogaea
-
 Valencia peanut
-
3.5.1.67 Arachis hypogaea
-
 peanut, groundnut
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.67 from fresh or frozen leaves Arachis hypogaea
3.5.1.67 multiple electrophoretically distinct forms Arachis hypogaea
3.5.1.67 partial Arachis hypogaea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.67 cotyledon 27% of total activity Arachis hypogaea
-
3.5.1.67 hypocotyl 2% of total activity Arachis hypogaea
-
3.5.1.67 leaf 14- to 16-day germinated peanuts Arachis hypogaea
-
3.5.1.67 leaf 68% of total activity Arachis hypogaea
-
3.5.1.67 additional information tissue distribution, not in root, imbibed seeds and mature plants Arachis hypogaea
-
3.5.1.67 additional information level of 4-methylene-L-glutamine amidohydrolase activity increases rapidly with germination to a maximum after 3 weeks Arachis hypogaea
-
3.5.1.67 stem 3% of total activity Arachis hypogaea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.67 49.3 106 3 multiple forms of 4-methylene-L-glutamine amidohydrolase: AH-1: 49.3 micromol/min/mg, AH-2: 106.0 micromol/min/mg, AH-3: 77.3 micromol/min/mg Arachis hypogaea

Storage Stability

EC Number Storage Stability Organism
3.5.1.67 4°C, stable for at least 4 months Arachis hypogaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.67 4-methyl-L-glutamine + H2O about 9% of the rate of 4-methylene-L-glutamine hydrolysis Arachis hypogaea 4-methyl-L-glutamate + NH3
-
 ?
3.5.1.67 4-methylene-L-glutamine + H2O highly specific Arachis hypogaea 4-methylene-L-glutamate + NH3
-
 ?
3.5.1.67 4-methylene-L-glutamine + H2O highly specific Arachis hypogaea ?
-
 ?
3.5.1.67 4-methylene-L-glutamine + H2O implicated in the process of N-translocation Arachis hypogaea ?
-
 ?
3.5.1.67 4-methylene-L-glutamine + H2O initial enzyme for catalyzing 4-methylene-L-glutamine degradation Arachis hypogaea ?
-
 ?
3.5.1.67 4-methylene-L-glutamine + H2O implicated in the transport of nitrogen to the developing shoots and leaves of germinating peanuts, involved in releasing nitrogen to the plants Arachis hypogaea ?
-
 ?
3.5.1.67 L-glutamine + H2O about 20% of the rate of 4-methylene-L-glutamine hydrolysis Arachis hypogaea L-glutamate + NH3
-
 ?
3.5.1.67 additional information substrate specificity Arachis hypogaea ?
-
 ?
3.5.1.67 additional information 5-carbon amino acid amide structure is essential for activity Arachis hypogaea ?
-
 ?
3.5.1.67 additional information L-asparagine: not a substrate Arachis hypogaea ?
-
 ?
3.5.1.67 additional information glutaramic acid, glutarimide, acrylamide, methacrylamide, succinamic acid, acetamide, urea, N-benzoyl-4-methylene-L-glutamine, isoglutamine, 4-methyleneisoglutamine: no substrates Arachis hypogaea ?
-
 ?
3.5.1.67 additional information implicated in the process of N-translocation Arachis hypogaea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.67 monomer 1 * 48600, SDS-PAGE Arachis hypogaea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.67 37
-
 assay at Arachis hypogaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.67 8 9 broad pH optimum Arachis hypogaea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.67 6.5 10.5 pH 6.5: 47% of maximal activity, pH 10.5: 27% of maximal activity Arachis hypogaea