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Literature summary extracted from
- beta-Ureidopropionase with N-carbamoyl-alpha-L-amino acid amidohydrolase activity from an aerobic bacterium, Pseudomonas putida IFO 12996 (1994), Eur. J. Biochem., 223, 625-630.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.6 | 2,2'-dipyridyl | 2 mM 70% inhibition | Pseudomonas putida |  |
| 3.5.1.6 | 5,5'-dithiobis-(2-nitrobenzoic acid) | - |
Pseudomonas putida | |
| 3.5.1.6 | 8-hydroxyquinoline | 2 mM 100% inhibition | Pseudomonas putida | |
| 3.5.1.6 | Ag+ | - |
Pseudomonas putida | |
| 3.5.1.6 | beta-Alanine | product inhibition | Pseudomonas putida | |
| 3.5.1.6 | beta-Alanine | product inhibition | Rattus rattus | |
| 3.5.1.6 | Cd2+ | - |
Pseudomonas putida | |
| 3.5.1.6 | Cu2+ | - |
Euglena gracilis | |
| 3.5.1.6 | Cu2+ | - |
Pseudomonas putida | |
| 3.5.1.6 | ethylendiaminetetraacetate | 2 mM 100% inhibition | Pseudomonas putida | |
| 3.5.1.6 | Hg2+ | - |
Pseudomonas putida | |
| 3.5.1.6 | N-carbamoyl-D-alanine | - |
Pseudomonas putida | |
| 3.5.1.6 | N-carbamoyl-L-leucine | - |
Pseudomonas putida | |
| 3.5.1.6 | N-ethylmaleimide | - |
Pseudomonas putida | |
| 3.5.1.6 | o-phenanthroline | 2 mM 93% inhibition | Pseudomonas putida | |
| 3.5.1.6 | p-chloromercuribenzoic acid | - |
Pseudomonas putida | |
| 3.5.1.6 | Pb2+ | - |
Pseudomonas putida | |
| 3.5.1.6 | phenylhydrazine | 49% inhibition | Pseudomonas putida | |
| 3.5.1.6 | Semicarbazide | 78% inhibition | Pseudomonas putida | |
| 3.5.1.6 | Sn2+ | - |
Pseudomonas putida | |
| 3.5.1.6 | sodium propionate | - |
Pseudomonas putida | |
| 3.5.1.6 | sodium propionate | - |
Rattus rattus | |
| 3.5.1.6 | Zn2+ | - |
Pseudomonas putida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.6 | 0.68 | - |
N-Carbamoylglycine | - |
Pseudomonas putida | |
| 3.5.1.6 | 1.56 | - |
N-Carbamoyl-beta-alanine | - |
Pseudomonas putida | |
| 3.5.1.6 | 2.81 | - |
N-carbamoyl-beta-aminoisobutyric acid | - |
Pseudomonas putida | |
| 3.5.1.6 | 3.74 | - |
beta-ureidopropionic acid | - |
Pseudomonas putida | |
| 3.5.1.6 | 4.52 | - |
DL-beta-ureidobutyric acid | - |
Pseudomonas putida | |
| 3.5.1.6 | 7.71 | - |
N-formyl-DL-alanine | - |
Pseudomonas putida | |
| 3.5.1.6 | 8.78 | - |
N-acetyl-DL-alanine | - |
Pseudomonas putida | |
| 3.5.1.6 | 11.6 | - |
gamma-ureido-n-butyrate | - |
Pseudomonas putida | |
| 3.5.1.6 | 42.3 | - |
N-carbamoyl-DL-2-aminovaleric acid | - |
Pseudomonas putida | |
| 3.5.1.6 | 75.1 | - |
N-carbamoyl-L-serine | - |
Pseudomonas putida | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.1.6 | cytosol | - |
Pseudomonas putida | 5829 | - |
| 3.5.1.6 | cytosol | - |
Rattus rattus | 5829 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.6 | Mg2+ | - |
Euglena gracilis | |
| 3.5.1.6 | Mg2+ | - |
Pseudomonas putida | |
| 3.5.1.6 | Ni2+ | - |
Pseudomonas putida | |
| 3.5.1.6 | Zn2+ | - |
Pseudomonas putida | |
| 3.5.1.6 | Zn2+ | - |
Rattus rattus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.6 | 42000 | - |
6 * 42000, SDS-PAGE | Rattus rattus |
| 3.5.1.6 | 45000 | - |
2 * 45000, SDS-PAGE | Pseudomonas putida |
| 3.5.1.6 | 95000 | - |
gel filtration | Pseudomonas putida |
| 3.5.1.6 | 240000 | - |
gel filtration | Rattus rattus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.6 | N-carbamoyl-beta-alanine + H2O | Mus musculus | - |
? | - |
? | |
| 3.5.1.6 | N-carbamoyl-beta-alanine + H2O | Euglena gracilis | - |
? | - |
? | |
| 3.5.1.6 | N-carbamoyl-beta-alanine + H2O | Pseudomonas putida | - |
? | - |
? | |
| 3.5.1.6 | N-carbamoyl-beta-alanine + H2O | Comamonas sp. | - |
? | - |
? | |
| 3.5.1.6 | N-carbamoyl-beta-alanine + H2O | Rattus rattus | - |
? | - |
? | |
| 3.5.1.6 | N-carbamoyl-beta-alanine + H2O | Clostridium uracilicum | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.6 | Clostridium uracilicum | - |
- |
- |
| 3.5.1.6 | Comamonas sp. | - |
- |
- |
| 3.5.1.6 | Euglena gracilis | - |
plant-like unicellular flagellate | - |
| 3.5.1.6 | Mus musculus | - |
- |
- |
| 3.5.1.6 | Pseudomonas putida | - |
IFO 12996 | - |
| 3.5.1.6 | Rattus rattus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.6 | - |
Pseudomonas putida |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.1.6 | liver | - |
Mus musculus | - |
| 3.5.1.6 | liver | - |
Rattus rattus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.6 | DL-beta-ureidobutyric acid + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 3.5.1.6 | gamma-ureido-n-butyric acid + H2O | - |
Pseudomonas putida | 4-aminobutanoate + CO2 + NH3 | - |
? | |
| 3.5.1.6 | N-acetyl-DL-alanine + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 3.5.1.6 | N-carbamoyl-beta-alanine + H2O | - |
Comamonas sp. | beta-alanine + CO2 + NH3 | - |
ir | |
| 3.5.1.6 | N-carbamoyl-beta-alanine + H2O | L enantiomer specific hydrolysis | Mus musculus | beta-alanine + CO2 + NH3 | - |
ir | |
| 3.5.1.6 | N-carbamoyl-beta-alanine + H2O | L enantiomer specific hydrolysis | Euglena gracilis | beta-alanine + CO2 + NH3 | - |
ir | |
| 3.5.1.6 | N-carbamoyl-beta-alanine + H2O | L enantiomer specific hydrolysis | Pseudomonas putida | beta-alanine + CO2 + NH3 | - |
ir | |
| 3.5.1.6 | N-carbamoyl-beta-alanine + H2O | L enantiomer specific hydrolysis | Rattus rattus | beta-alanine + CO2 + NH3 | - |
ir | |
| 3.5.1.6 | N-carbamoyl-beta-alanine + H2O | L enantiomer specific hydrolysis | Clostridium uracilicum | beta-alanine + CO2 + NH3 | - |
ir | |
| 3.5.1.6 | N-carbamoyl-beta-alanine + H2O | - |
Mus musculus | ? | - |
? | |
| 3.5.1.6 | N-carbamoyl-beta-alanine + H2O | - |
Euglena gracilis | ? | - |
? | |
| 3.5.1.6 | N-carbamoyl-beta-alanine + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 3.5.1.6 | N-carbamoyl-beta-alanine + H2O | - |
Comamonas sp. | ? | - |
? | |
| 3.5.1.6 | N-carbamoyl-beta-alanine + H2O | - |
Rattus rattus | ? | - |
? | |
| 3.5.1.6 | N-carbamoyl-beta-alanine + H2O | - |
Clostridium uracilicum | ? | - |
? | |
| 3.5.1.6 | N-carbamoyl-DL-2-aminovaleric acid + H2O | - |
Pseudomonas putida | 2-aminopentanoate + CO2 + NH3 | - |
? | |
| 3.5.1.6 | N-carbamoyl-DL-beta-aminoisobutyrate + H2O | - |
Euglena gracilis | DL-beta-aminoisobutyrate + CO2 + NH3 | - |
? | |
| 3.5.1.6 | N-carbamoyl-DL-beta-aminoisobutyrate + H2O | - |
Pseudomonas putida | DL-beta-aminoisobutyrate + CO2 + NH3 | - |
? | |
| 3.5.1.6 | N-carbamoyl-DL-beta-aminoisobutyrate + H2O | - |
Rattus rattus | DL-beta-aminoisobutyrate + CO2 + NH3 | - |
? | |
| 3.5.1.6 | N-carbamoyl-L-serine + H2O | - |
Pseudomonas putida | L-serine + CO2 + NH3 | - |
? | |
| 3.5.1.6 | N-carbamoylglycine + H2O | - |
Pseudomonas putida | glycine + CO2 + NH3 | - |
? | |
| 3.5.1.6 | N-carbamoylglycine + H2O | - |
Rattus rattus | glycine + CO2 + NH3 | - |
? | |
| 3.5.1.6 | N-formyl-DL-alanine + H2O | - |
Pseudomonas putida | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.6 | dimer | 2 * 45000, SDS-PAGE | Pseudomonas putida |
| 3.5.1.6 | hexamer | 6 * 42000, SDS-PAGE | Rattus rattus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.6 | 60 | - |
- |
Euglena gracilis |
| 3.5.1.6 | 60 | - |
- |
Pseudomonas putida |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.6 | 65 | - |
80% activity lost at 70°C | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.6 | 7.5 | 8.2 | - |
Pseudomonas putida |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.6 | 6.1 | 10.7 | - |
Pseudomonas putida |
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