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Literature summary extracted from

  • Kanagawa, K.; Oishi, M.; Negoro, S.; Urabe, I.; Okada, H.
    Characterization of the 6-aminohexanoate-dimer hydrolase from Pseudomonas sp. NK87 (1993), J. Gen. Microbiol., 139, 787-795.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.46 expression in Escherichia coli Pseudomonas sp.
3.5.1.46 expression in Escherichia coli Flavobacterium sp.
3.5.2.12 overexpression in Escherichia coli Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.46 diisopropyl fluorophosphate
-
 Flavobacterium sp.
3.5.1.46 diisopropyl fluorophosphate 80% inhibition at 1 mM Pseudomonas sp.
3.5.2.12 diisopropylphosphofluoridate
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.46 0.6
-
 N-(6-aminohexanoyl)-6-aminohexanoate
-
 Pseudomonas sp.
3.5.1.46 15
-
 N-(6-aminohexanoyl)-6-aminohexanoate
-
 Flavobacterium sp.
3.5.2.12 0.6
-
 1,8-Diazacyclotetradecane-2,9-dione
-
 Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.46 intracellular
-
 Pseudomonas sp. 5622
-
3.5.1.46 intracellular
-
 Flavobacterium sp. 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.46 42000
-
 2 * 42000, SDS-PAGE Pseudomonas sp.
3.5.1.46 42000
-
 2 * 42000, SDS-PAGE Flavobacterium sp.
3.5.1.46 76000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.12 1,8-diazacyclotetradecane-2,9-dione + H2O Pseudomonas sp.
-
 N-(6-aminohexanoyl)-6-aminohexanoate
-
 ?
3.5.2.12 1,8-diazacyclotetradecane-2,9-dione + H2O Pseudomonas sp. NK87
-
 N-(6-aminohexanoyl)-6-aminohexanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.46 Flavobacterium sp.
-
-
-
3.5.1.46 Flavobacterium sp. KI72
-
-
-
3.5.1.46 Pseudomonas sp.
-
-
-
3.5.1.46 Pseudomonas sp. NK87
-
-
-
3.5.2.12 Pseudomonas sp.
-
 NK87
-
3.5.2.12 Pseudomonas sp. NK87
-
 NK87
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.46 to homogeneity, chromatography techniques, recombinant enzyme Pseudomonas sp.
3.5.1.46 to homogeneity, chromatography techniques, recombinant enzyme Flavobacterium sp.
3.5.2.12
-
 Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.46 cell culture
-
 Pseudomonas sp.
-
3.5.1.46 cell culture
-
 Flavobacterium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.46 2.7
-
 purified from parental strain Pseudomonas sp.
3.5.1.46 4
-
 purified from cloned Escherichia coli strain Pseudomonas sp.
3.5.2.12 0.004
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.46 N-(6-aminohexanoyl)-6-aminohexanoate + H2O
-
 Pseudomonas sp. 6-aminohexanoate + 6-aminohexanoate
-
 ?
3.5.1.46 N-(6-aminohexanoyl)-6-aminohexanoate + H2O
-
 Flavobacterium sp. 6-aminohexanoate + 6-aminohexanoate
-
 ?
3.5.1.46 N-(6-aminohexanoyl)-6-aminohexanoate + H2O
-
 Flavobacterium sp. KI72 6-aminohexanoate + 6-aminohexanoate
-
 ?
3.5.1.46 N-(6-aminohexanoyl)-6-aminohexanoate + H2O
-
 Pseudomonas sp. NK87 6-aminohexanoate + 6-aminohexanoate
-
 ?
3.5.1.46 N-6-aminohexanoate pentamer + H2O
-
 Pseudomonas sp. 6-aminohexanoate + ?
-
 ?
3.5.1.46 N-6-aminohexanoate pentamer + H2O
-
 Flavobacterium sp. 6-aminohexanoate + ?
-
 ?
3.5.1.46 N-6-aminohexanoate pentamer + H2O
-
 Flavobacterium sp. KI72 6-aminohexanoate + ?
-
 ?
3.5.1.46 N-6-aminohexanoate pentamer + H2O
-
 Pseudomonas sp. NK87 6-aminohexanoate + ?
-
 ?
3.5.1.46 N-6-aminohexanoate tetramer + H2O
-
 Flavobacterium sp. 6-aminohexanoate + N-6-aminohexanoate trimer
-
 ?
3.5.1.46 N-6-aminohexanoate tetramer + H2O highest relative activity Pseudomonas sp. 6-aminohexanoate + N-6-aminohexanoate trimer major products, the trimer is subsequently subjected to hydrolysis, digests exogenously linear oligomers from the N-terminus as monomeric units ?
3.5.1.46 N-6-aminohexanoate tetramer + H2O
-
 Flavobacterium sp. KI72 6-aminohexanoate + N-6-aminohexanoate trimer
-
 ?
3.5.1.46 N-6-aminohexanoate tetramer + H2O highest relative activity Pseudomonas sp. NK87 6-aminohexanoate + N-6-aminohexanoate trimer major products, the trimer is subsequently subjected to hydrolysis, digests exogenously linear oligomers from the N-terminus as monomeric units ?
3.5.1.46 N-6-aminohexanoate trimer + H2O
-
 Pseudomonas sp. 6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
 ?
3.5.1.46 N-6-aminohexanoate trimer + H2O
-
 Flavobacterium sp. 6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
 ?
3.5.1.46 N-6-aminohexanoate trimer + H2O
-
 Flavobacterium sp. KI72 6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
 ?
3.5.1.46 N-6-aminohexanoate trimer + H2O
-
 Pseudomonas sp. NK87 6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
 ?
3.5.2.12 1,8-diazacyclotetradecane-2,9-dione + H2O
-
 Pseudomonas sp. N-(6-aminohexanoyl)-6-aminohexanoate
-
 ?
3.5.2.12 1,8-diazacyclotetradecane-2,9-dione + H2O i.e. 6-aminohexanoic acid cyclic dimer Pseudomonas sp. N-(6-aminohexanoyl)-6-aminohexanoate
-
 ?
3.5.2.12 1,8-diazacyclotetradecane-2,9-dione + H2O
-
 Pseudomonas sp. NK87 N-(6-aminohexanoyl)-6-aminohexanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.46 dimer 2 * 42000, SDS-PAGE Pseudomonas sp.
3.5.1.46 dimer 2 * 42000, SDS-PAGE Flavobacterium sp.

Synonyms

EC Number Synonyms Comment Organism
3.5.1.46 EII
-
 Pseudomonas sp.
3.5.1.46 EII
-
 Flavobacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.46 40
-
-
 Flavobacterium sp.
3.5.1.46 48
-
-
 Pseudomonas sp.
3.5.2.12 48
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.46 45
-
 stable up to Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.46 9.2
-
 N-(6-aminohexanoyl)-6-aminohexanoate
-
 Pseudomonas sp.
3.5.1.46 19
-
 N-(6-aminohexanoyl)-6-aminohexanoate
-
 Flavobacterium sp.
3.5.2.12 10.7
-
 1,8-Diazacyclotetradecane-2,9-dione
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.46 7.5
-
-
 Pseudomonas sp.
3.5.1.46 9
-
-
 Flavobacterium sp.
3.5.2.12 7.5
-
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.46 6.8 8.5 stable within Pseudomonas sp.