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Literature summary extracted from

  • Reitz, M.S.; Rodwell, V.W.
    delta-Aminovaleramidase of Pseudomonas putida (1970), J. Biol. Chem., 245, 3091-3096.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.30 dithioerythritol effectively reverses heavy metal inactivation Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.30 AgNO3 0.00125 mM, 100% inhibition Pseudomonas putida
3.5.1.30 arsenite characteristic of enzymes with essential vicinal sulfhydryl groups Pseudomonas putida
3.5.1.30 Ba2+ 5 mM, inhibition from 10-40% Pseudomonas putida
3.5.1.30 Ca2+ 5 mM, inhibition from 10-40% Pseudomonas putida
3.5.1.30 Cd(acetate)2 0.00125 mM, 94% inhibition Pseudomonas putida
3.5.1.30 Cd2+ 0.5 mM, complete inhibition , characteristic of enzymes with essential vicinal sulfhydryl groups Pseudomonas putida
3.5.1.30 Co2+ 5.0 mM,75% inhibition Pseudomonas putida
3.5.1.30 Cu2+ 5.0 mM, complete inhibition Pseudomonas putida
3.5.1.30 Fe2+ 5 mM, inhibition from 10-40% Pseudomonas putida
3.5.1.30 Hg(NO3)2 0.187 mM, 100% inhibition Pseudomonas putida
3.5.1.30 Hg2+ 0.5 mM, complete inhibition Pseudomonas putida
3.5.1.30 HgCl2 0.00125 mM, 100% inhibition Pseudomonas putida
3.5.1.30 Mg2+ 5 mM, inhibition from 10-40% Pseudomonas putida
3.5.1.30 NaAsO2 0.0125 mM, 47% inhibition Pseudomonas putida
3.5.1.30 p-hydroxymercuribenzoate 0.00025 mM, 44% inhibition, inhibition partially reversed by dithioerythritol Pseudomonas putida
3.5.1.30 Pb2+ 5.0 mM, complete inhibition Pseudomonas putida
3.5.1.30 Sn2+ 5.0 mM, complete inhibition Pseudomonas putida
3.5.1.30 Zn2+ 0.5 mM, complete inhibition Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.30 12
-
 6-Aminohexanamide
-
 Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.30 cytoplasm
-
 Pseudomonas putida 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.30 Al3+ 0.125 mM stimulates activity 37% Pseudomonas putida
3.5.1.30 Fe3+ 0.125 mM stimulates activity 25% Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.30 67000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.30 5-aminopentanamide + H2O Pseudomonas putida known intermediate in lysine catabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.30 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.30 purified 400fold from the soluble fraction of cell-free extract Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.30 130
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.30 5-aminopentanamide + H2O
-
 Pseudomonas putida 5-aminopentanoate + NH3
-
 ?
3.5.1.30 5-aminopentanamide + H2O known intermediate in lysine catabolism Pseudomonas putida ?
-
 ?
3.5.1.30 6-aminohexanamide + H2O
-
 Pseudomonas putida 6-aminohexanoate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.30 delta-aminovaleramidase
-
 Pseudomonas putida
3.5.1.30 delta-aminovaleramide amidohydrolase
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.30 7.5 8.5
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.30 7 9 stable below pH 6.5, irreversibly inactivated above pH 9 Pseudomonas putida