EC Number | General Stability | Organism |
---|---|---|
3.5.1.29 | stable at room temperature between pH 6 and 10 | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.29 | alpha-hydroxyglutarate | competitive inhibition | Pseudomonas sp. | |
3.5.1.29 | alpha-ketoglutarate | - |
Arthrobacter sp. | |
3.5.1.29 | alpha-ketoglutarate | competitive inhibition | Pseudomonas sp. | |
3.5.1.29 | citrate | competitive inhibition | Pseudomonas sp. | |
3.5.1.29 | diphosphate | - |
Arthrobacter sp. | |
3.5.1.29 | diphosphate | competitive inhibition | Pseudomonas sp. | |
3.5.1.29 | fumarate | - |
Arthrobacter sp. | |
3.5.1.29 | fumarate | competitive inhibition | Pseudomonas sp. | |
3.5.1.29 | glutamate | competitive inhibition | Pseudomonas sp. | |
3.5.1.29 | Glutarate | competitive inhibition | Pseudomonas sp. | |
3.5.1.29 | Hg2+ | - |
Pseudomonas sp. | |
3.5.1.29 | Itaconate | competitively inhibited by di-and tricarboxylic acids, itaconic acid being most effective, monocarboxylic acids do not inhibit | Arthrobacter sp. | |
3.5.1.29 | Itaconate | competitively inhibited by di-and tricarboxylic acids, itaconic acid being most effective, monocarboxylic acids do not inhibit | Pseudomonas sp. | |
3.5.1.29 | malate | competitive inhibition | Pseudomonas sp. | |
3.5.1.29 | Maleate | competitive inhibition | Pseudomonas sp. | |
3.5.1.29 | malonate | competitive inhibition | Pseudomonas sp. | |
3.5.1.29 | N-acetyl-DL-aspartate | - |
Arthrobacter sp. | |
3.5.1.29 | N-acetyl-DL-aspartate | competitive inhibition | Pseudomonas sp. | |
3.5.1.29 | oxaloacetate | - |
Arthrobacter sp. | |
3.5.1.29 | oxaloacetate | competitive inhibition | Pseudomonas sp. | |
3.5.1.29 | phosphate | - |
Arthrobacter sp. | |
3.5.1.29 | phosphate | competitive inhibition | Pseudomonas sp. | |
3.5.1.29 | SO32- | inhibitory effect increases rapidly with time to a constant level, not competitve with substrate | Arthrobacter sp. | |
3.5.1.29 | SO32- | inhibitory effect increases rapidly with time to a constant level, not competitve with substrate | Pseudomonas sp. | |
3.5.1.29 | succinate | - |
Arthrobacter sp. | |
3.5.1.29 | succinate | competitive inhibition | Pseudomonas sp. | |
3.5.1.66 | Dicarboxylic acids | competitive | Arthrobacter sp. | |
3.5.1.66 | diphosphate | competitive | Arthrobacter sp. | |
3.5.1.66 | Hg2+ | 0.01 mM, 15 min, 82% activity remains | Arthrobacter sp. | |
3.5.1.66 | Itaconic acid | competitive, most effective inhibitor, Ki: 0.74 mM | Arthrobacter sp. | |
3.5.1.66 | malonate | competitive, Ki: 20 mM | Arthrobacter sp. | |
3.5.1.66 | additional information | no inhibition by: organic protein reagents, 0.1 mM, 15 min, p-chloromercuribenzoate, 1.0 mM Ni2+, 1.0 mM Co2+, 1.0 mM Mn2+, 1.0 mM Mg2+, 1.0 mM Ca2+, 1.0 mM Cu2+, 1.0 mM Cs+, 1.0 mM Li+, SO42-, NO3-, Cl-, 10 mM dithiothreitol, 100 mM monocarboxylic acids | Arthrobacter sp. | |
3.5.1.66 | phosphate | competitive | Arthrobacter sp. | |
3.5.1.66 | sulfite | noncompetitive, inhibition by a time-dependent mechanism, addition of succinic semialdehyde protects against sulfite inhibition | Arthrobacter sp. | |
3.5.1.66 | Tricarboxylic acids | competitive | Arthrobacter sp. | |
3.5.1.66 | Zn2+ | 1 mM: 40% inhibition | Arthrobacter sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.29 | 0.003 | - |
2-(Acetamidomethylene)succinate | at pH 6.5 and 25°C | Pseudomonas sp. | |
3.5.1.66 | additional information | - |
additional information | kinetic data | Arthrobacter sp. | |
3.5.1.66 | 0.025 | - |
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate | compound B, 25°C, Tris-HCl, pH 7.0 | Arthrobacter sp. | |
3.5.1.66 | 0.123 | - |
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate | compound B, 2-(N-morpholino)ethane-sulfonic acid, pH 6.0 | Arthrobacter sp. | |
3.5.1.66 | 0.25 | - |
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate | compound B, Tris-HCl, pH 8.0 | Arthrobacter sp. | |
3.5.1.66 | 0.56 | - |
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate | compound B, 2-(N-morpholino)ethane-sulfonic acid, pH 6.5 | Arthrobacter sp. | |
3.5.1.66 | 0.68 | - |
3-(acetamidomethylene)succinate | compound A, 25°C, pH 7.0 | Arthrobacter sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.66 | additional information | some inorganic cations activate | Arthrobacter sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.29 | 32500 | - |
gel filtration | Pseudomonas sp. |
3.5.1.66 | 36600 | - |
Cr-7, probably six identical subunits, 6 * 36600, SDS-PAGE | Arthrobacter sp. |
3.5.1.66 | 205000 | - |
Cr-7, gel filtration | Arthrobacter sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.29 | 2-(acetamidomethylene)succinate + 2 H2O | Pseudomonas sp. | - |
acetate + succinate semialdehyde + NH3 + CO2 | - |
? | |
3.5.1.29 | 2-(acetamidomethylene)succinate + 2 H2O | Pseudomonas sp. IA | - |
acetate + succinate semialdehyde + NH3 + CO2 | - |
? | |
3.5.1.66 | 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O | Pseudomonas sp. | compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid | ? | - |
? | |
3.5.1.66 | 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O | Arthrobacter sp. | compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid | ? | - |
? | |
3.5.1.66 | 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O | Arthrobacter sp. | involved in pyridoxine degradation | ? | - |
? | |
3.5.1.66 | 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O | Pseudomonas sp. IA | compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid | ? | - |
? | |
3.5.1.66 | additional information | Pseudomonas sp. | enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen | ? | - |
? | |
3.5.1.66 | additional information | Arthrobacter sp. | enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen | ? | - |
? | |
3.5.1.66 | additional information | Pseudomonas sp. IA | enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.29 | Arthrobacter sp. | - |
Cr-7 | - |
3.5.1.29 | Pseudomonas sp. | - |
MA-1 | - |
3.5.1.66 | Arthrobacter sp. | - |
Cr-7 | - |
3.5.1.66 | Pseudomonas sp. | - |
IA | - |
3.5.1.66 | Pseudomonas sp. IA | - |
IA | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.29 | - |
Pseudomonas sp. |
3.5.1.66 | Cr-7 | Arthrobacter sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.29 | 3.12 | - |
- |
Pseudomonas sp. |
3.5.1.66 | additional information | - |
- |
Arthrobacter sp. |
3.5.1.66 | 3.79 | - |
25°C | Arthrobacter sp. |
EC Number | Storage Stability | Organism |
---|---|---|
3.5.1.66 | Cr-7, -20°C, 0.1 M Tris-HCl, pH 7.0, stable for several months | Arthrobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.29 | 2-(acetamidomethylene)succinate + 2 H2O | - |
Pseudomonas sp. | acetate + succinate semialdehyde + NH3 + CO2 | - |
? | |
3.5.1.29 | 2-(acetamidomethylene)succinate + 2 H2O | - |
Pseudomonas sp. | acetate + succinate semialdehyde + NH3 + CO2 | same products as formed by acid hydrolysis | ir | |
3.5.1.29 | 2-(acetamidomethylene)succinate + 2 H2O | - |
Pseudomonas sp. IA | acetate + succinate semialdehyde + NH3 + CO2 | - |
? | |
3.5.1.29 | 2-(acetamidomethylene)succinate + 2 H2O | - |
Pseudomonas sp. IA | acetate + succinate semialdehyde + NH3 + CO2 | same products as formed by acid hydrolysis | ir | |
3.5.1.66 | 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O | compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid | Pseudomonas sp. | acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2 | - |
? | |
3.5.1.66 | 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O | compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid | Arthrobacter sp. | acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2 | - |
? | |
3.5.1.66 | 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O | compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid | Pseudomonas sp. IA | acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2 | - |
? | |
3.5.1.66 | 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O | compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid | Pseudomonas sp. | ? | - |
? | |
3.5.1.66 | 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O | compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid | Arthrobacter sp. | ? | - |
? | |
3.5.1.66 | 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O | involved in pyridoxine degradation | Arthrobacter sp. | ? | - |
? | |
3.5.1.66 | 2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O | compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid | Pseudomonas sp. IA | ? | - |
? | |
3.5.1.66 | 3-(acetamidomethylene)succinate + H2O | compound A, alpha-(N-acetylaminomethylene)succinic acid: poor substrate | Arthrobacter sp. | acetate + 4-oxobutanoate + NH3 + CO2 | - |
? | |
3.5.1.66 | additional information | high substrate specificity | Arthrobacter sp. | ? | - |
? | |
3.5.1.66 | additional information | enzyme highly specific, inducible amidohydrolase | Arthrobacter sp. | ? | - |
? | |
3.5.1.66 | additional information | enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen | Pseudomonas sp. | ? | - |
? | |
3.5.1.66 | additional information | enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen | Arthrobacter sp. | ? | - |
? | |
3.5.1.66 | additional information | enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen | Pseudomonas sp. IA | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.29 | monomer | - |
Arthrobacter sp. |
3.5.1.29 | monomer | SDS-PAGE | Pseudomonas sp. |
3.5.1.66 | multimer | Cr-7, probably six identical subunits, 6 * 36600, SDS-PAGE | Arthrobacter sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.29 | compound A hydrolase | - |
- |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.29 | 25 | - |
- |
Pseudomonas sp. |
3.5.1.66 | 25 | - |
assay at | Arthrobacter sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.29 | 70 | 90 | at pH 8 and 70°C stable for 1 min, loses activity completely in 1 min at 90°C | Pseudomonas sp. |
3.5.1.66 | 70 | - |
pH 8.0, 1 min, stable at | Arthrobacter sp. |
3.5.1.66 | 100 | - |
1 min, 65% activity retained | Arthrobacter sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.29 | 1.67 | - |
2-(Acetamidomethylene)succinate | - |
Pseudomonas sp. | |
3.5.1.66 | 2.3 | - |
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate | turnover no./subunit, 25°C | Arthrobacter sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.29 | 6.5 | 7.5 | pH 6.5 in 50 mM sodium phosphate, MES and HEPES buffers, pH 7.5 in Tris buffer | Pseudomonas sp. |
3.5.1.66 | additional information | - |
pI: 4.2, effect of buffer on pH optimum | Arthrobacter sp. |
3.5.1.66 | 6 | 7 | - |
Arthrobacter sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.29 | 6 | 10 | most efficient between pH 6.0 and 7.0 | Pseudomonas sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.29 | 6 | 10 | loses activity rapidly between 4.0 and 5.0 | Pseudomonas sp. |
3.5.1.66 | 4 | 5 | rapid loss of activity | Arthrobacter sp. |
3.5.1.66 | 6 | 10 | stable at room temperature | Arthrobacter sp. |
3.5.1.66 | 8 | - |
70°C, 1 min, stable at | Arthrobacter sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.66 | 0.74 | - |
Itaconic acid | competitive, most effective inhibitor | Arthrobacter sp. | |
3.5.1.66 | 20 | - |
malonate | competitive | Arthrobacter sp. |