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Literature summary extracted from

  • Singer, H.J.; Wise, E.M.; Park, J.T.
    Properties and purification of N-acetylmuramyl-L-alanine amidase from Staphylococcus aureus H (1972), J. Bacteriol., 112, 932-939.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.28 ammonium acetate 18% activation at 0.15 M Staphylococcus aureus
3.5.1.28 ammonium sulfate 20% activation at 0.15 M Staphylococcus aureus
3.5.1.28 NH4Cl 8% activation at 0.15 M Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.28 Cu2+
-
 Staphylococcus aureus
3.5.1.28 EDTA
-
 Staphylococcus aureus
3.5.1.28 K+
-
 Staphylococcus aureus
3.5.1.28 Na+
-
 Staphylococcus aureus
3.5.1.28 p-chloromercuribenzene sulfonate
-
 Staphylococcus aureus
3.5.1.28 Pb2+
-
 Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.28 800000 1000000 sucrose density gradient centrifugation, gel filtration Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.28 Staphylococcus aureus
-
-
-

Storage Stability

EC Number Storage Stability Organism
3.5.1.28 -20°C or 4°C, overnight, in glass tubes, total loss of activity Staphylococcus aureus
3.5.1.28 -20°C, crude cell extract, 2 years, 20% loss of activity Staphylococcus aureus
3.5.1.28 -20°C, overnight, in polycarbonate tubes, 25-50% loss of activity Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.28 murein + H2O also murein extracted from cells grown in the presence of penicillin, N-acetylated murein Staphylococcus aureus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.28 25
-
 10 min at 25°C result in 25% loss of inactivation Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.28 7.3
-
-
 Staphylococcus aureus