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Literature summary extracted from

  • Okino, N.; Tani, M.; Imayama, S.; Ito, M.
    Purification and characterization of a novel ceramidase from Pseudomonas aeruginoa (1998), J. Biol. Chem., 273, 14368-14373.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.23 Mg2+
-
 Pseudomonas aeruginosa
3.5.1.23 Triton X-100
-
 Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.23 Cu2+
-
 Pseudomonas aeruginosa
3.5.1.23 EDTA
-
 Pseudomonas aeruginosa
3.5.1.23 EGTA
-
 Pseudomonas aeruginosa
3.5.1.23 Hg2+
-
 Pseudomonas aeruginosa
3.5.1.23 N-oleoylethanolamine
-
 Pseudomonas aeruginosa
3.5.1.23 phytosphingosine
-
 Pseudomonas aeruginosa
3.5.1.23 sphinganine
-
 Pseudomonas aeruginosa
3.5.1.23 sphingosine
-
 Pseudomonas aeruginosa
3.5.1.23 Zn2+
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.23 0.139
-
 N-palmitoylsphingosine
-
 Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.23 extracellular
-
 Pseudomonas aeruginosa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.23 Ca2+ after EDTA treatment, Ca2+ reestablishes activity completely Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.23 70000
-
 x * 70000, SDS-PAGE Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.23 83400fold Pseudomonas aeruginosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.23 culture filtrate
-
 Pseudomonas aeruginosa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.23 186
-
-
 Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
3.5.1.23 -85°C, 2 months, with 1 mg/ml bovine serum albumin, no loss of activity Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 7-nitrobenz-2-oxa-1,3-diazole-N-lauroylsphingosine + H2O
-
 Pseudomonas aeruginosa ?
-
 r
3.5.1.23 N-palmitoylphytosphingosine + H2O
-
 Pseudomonas aeruginosa ?
-
 r
3.5.1.23 N-palmitoylsphinganine + H2O
-
 Pseudomonas aeruginosa palmitic acid + sphinganine
-
 r
3.5.1.23 N-palmitoylsphingosine + H2O
-
 Pseudomonas aeruginosa palmitic acid + sphingosine
-
 r
3.5.1.23 N-stearoylphytosphingosine + H2O
-
 Pseudomonas aeruginosa ?
-
 r
3.5.1.23 N-stearoylsphinganine + H2O
-
 Pseudomonas aeruginosa stearic acid + sphinganine
-
 r
3.5.1.23 N-stearoylsphingosine + H2O
-
 Pseudomonas aeruginosa stearic acid + sphingosine
-
 r

Subunits

EC Number Subunits Comment Organism
3.5.1.23 ? x * 70000, SDS-PAGE Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.23 24
-
 stable at 24°C for 12 h Pseudomonas aeruginosa
3.5.1.23 37
-
 total loss of activity after 12 h at 37°C Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 8.5
-
-
 Pseudomonas aeruginosa