Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chibata, I.; Ishikawa, T.; Tosa, T.
    epsilon-lysine acylase from Achromobacter pestifer (1970), Methods Enzymol., 19, 756-762.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.17 Ag+
-
 Achromobacter pestifer
3.5.1.17 citrate slight inhibitory effect Achromobacter pestifer
3.5.1.17 diphosphate marked inhibition Achromobacter pestifer
3.5.1.17 EDTA slight inhibitory effect Achromobacter pestifer
3.5.1.17 Hg+
-
 Achromobacter pestifer
3.5.1.17 Hg2+
-
 Achromobacter pestifer
3.5.1.17 Monoiodoacetic acid
-
 Achromobacter pestifer
3.5.1.17 omega-chloroacetophenone
-
 Achromobacter pestifer
3.5.1.17 oxalate marked inhibition Achromobacter pestifer
3.5.1.17 p-chloromercuribenzoate inhibition is completely recovered by addition of cysteine Achromobacter pestifer

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.17 0.1
-
 N6-benzoyl-L-lysine
-
 Achromobacter pestifer
3.5.1.17 0.29
-
 N6-chloroacetyl-L-lysine
-
 Achromobacter pestifer
3.5.1.17 0.5
-
 N6-acetyl-L-lysine
-
 Achromobacter pestifer

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.17 epsilon-N-acyl-L-lysine + H2O Gallus gallus
-
 ?
-
 ?
3.5.1.17 epsilon-N-acyl-L-lysine + H2O Rattus norvegicus
-
 ?
-
 ?
3.5.1.17 epsilon-N-acyl-L-lysine + H2O Sus scrofa
-
 ?
-
 ?
3.5.1.17 epsilon-N-acyl-L-lysine + H2O Pseudomonas sp.
-
 ?
-
 ?
3.5.1.17 epsilon-N-acyl-L-lysine + H2O Mushroom
-
 ?
-
 ?
3.5.1.17 epsilon-N-acyl-L-lysine + H2O Aspergillus oryzae
-
 ?
-
 ?
3.5.1.17 epsilon-N-acyl-L-lysine + H2O Achromobacter pestifer
-
 ?
-
 ?
3.5.1.17 epsilon-N-acyl-L-lysine + H2O Columba sp.
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.17 Achromobacter pestifer
-
 EA ATCC 23584
-
3.5.1.17 Aspergillus oryzae
-
 No. 10, mould
-
3.5.1.17 Columba sp.
-
 pigeon
-
3.5.1.17 Gallus gallus
-
 chicken
-
3.5.1.17 Mushroom
-
-
-
3.5.1.17 Pseudomonas sp.
-
-
-
3.5.1.17 Rattus norvegicus
-
 rat
-
3.5.1.17 Sus scrofa
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.17
-
 Rattus norvegicus
3.5.1.17
-
 Sus scrofa
3.5.1.17
-
 Pseudomonas sp.
3.5.1.17
-
 Aspergillus oryzae
3.5.1.17
-
 Achromobacter pestifer

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.17 brain
-
 Gallus gallus
-
3.5.1.17 brain
-
 Rattus norvegicus
-
3.5.1.17 brain
-
 Sus scrofa
-
3.5.1.17 brain
-
 Columba sp.
-
3.5.1.17 heart
-
 Gallus gallus
-
3.5.1.17 heart
-
 Rattus norvegicus
-
3.5.1.17 heart
-
 Sus scrofa
-
3.5.1.17 heart
-
 Columba sp.
-
3.5.1.17 kidney richest source Gallus gallus
-
3.5.1.17 kidney richest source Rattus norvegicus
-
3.5.1.17 kidney richest source Sus scrofa
-
3.5.1.17 kidney richest source Columba sp.
-
3.5.1.17 liver
-
 Gallus gallus
-
3.5.1.17 liver
-
 Rattus norvegicus
-
3.5.1.17 liver
-
 Sus scrofa
-
3.5.1.17 liver
-
 Columba sp.
-
3.5.1.17 pancreas
-
 Gallus gallus
-
3.5.1.17 pancreas
-
 Rattus norvegicus
-
3.5.1.17 pancreas
-
 Sus scrofa
-
3.5.1.17 pancreas
-
 Columba sp.
-
3.5.1.17 spleen
-
 Gallus gallus
-
3.5.1.17 spleen
-
 Rattus norvegicus
-
3.5.1.17 spleen
-
 Sus scrofa
-
3.5.1.17 spleen
-
 Columba sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.17 14.5
-
 homogenous protein, vertical zone electrophoresis, purification 60.4fold Achromobacter pestifer
3.5.1.17 240
-
 cell-free extract, purification 1fold Achromobacter pestifer

Storage Stability

EC Number Storage Stability Organism
3.5.1.17 0°C, 60% ammonium sulfate, can be stored several months without decrease of activity Achromobacter pestifer

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.17 epsilon-N-acyl-L-lysine + H2O
-
 Gallus gallus ?
-
 ?
3.5.1.17 epsilon-N-acyl-L-lysine + H2O
-
 Rattus norvegicus ?
-
 ?
3.5.1.17 epsilon-N-acyl-L-lysine + H2O
-
 Sus scrofa ?
-
 ?
3.5.1.17 epsilon-N-acyl-L-lysine + H2O
-
 Pseudomonas sp. ?
-
 ?
3.5.1.17 epsilon-N-acyl-L-lysine + H2O
-
 Mushroom ?
-
 ?
3.5.1.17 epsilon-N-acyl-L-lysine + H2O
-
 Aspergillus oryzae ?
-
 ?
3.5.1.17 epsilon-N-acyl-L-lysine + H2O
-
 Achromobacter pestifer ?
-
 ?
3.5.1.17 epsilon-N-acyl-L-lysine + H2O
-
 Columba sp. ?
-
 ?
3.5.1.17 additional information enzyme is inducibly formed by N6-benzoyl-L-, -D-, or -DL-lysine, no optical specificity on hydrolysis of N6-acyl-DL-lysine, the differences indicate that the epsilon-lysine acylases from animal, mould and bacterial sources are different enzymes Aspergillus oryzae ?
-
 ?
3.5.1.17 N6-acetyl-L-lysine + H2O
-
 Gallus gallus L-lysine + acetate
-
 ?
3.5.1.17 N6-acetyl-L-lysine + H2O
-
 Rattus norvegicus L-lysine + acetate
-
 ?
3.5.1.17 N6-acetyl-L-lysine + H2O
-
 Sus scrofa L-lysine + acetate
-
 ?
3.5.1.17 N6-acetyl-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + acetate
-
 ?
3.5.1.17 N6-acyl-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + carboxylic acid
-
 ?
3.5.1.17 N6-alpha-naphthoyl-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + 1-naphthol
-
 ?
3.5.1.17 N6-benzoyl-L-lysine + H2O
-
 Gallus gallus L-lysine + benzoate
-
 ?
3.5.1.17 N6-benzoyl-L-lysine + H2O
-
 Rattus norvegicus L-lysine + benzoate
-
 ?
3.5.1.17 N6-benzoyl-L-lysine + H2O
-
 Sus scrofa L-lysine + benzoate
-
 ?
3.5.1.17 N6-benzoyl-L-lysine + H2O
-
 Pseudomonas sp. L-lysine + benzoate
-
 ?
3.5.1.17 N6-benzoyl-L-lysine + H2O
-
 Aspergillus oryzae L-lysine + benzoate
-
 ?
3.5.1.17 N6-benzoyl-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + benzoate
-
 ?
3.5.1.17 N6-benzoyl-L-lysine + H2O
-
 Columba sp. L-lysine + benzoate
-
 ?
3.5.1.17 N6-butyryl-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + butanoate
-
 ?
3.5.1.17 N6-caproyl-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + n-hexanoate
-
 ?
3.5.1.17 N6-caprylyl-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + caprylic acid
-
 ?
3.5.1.17 N6-carbobenzoxy-L-lysine + H2O
-
 Gallus gallus L-lysine + benzoate
-
 ?
3.5.1.17 N6-carbobenzoxy-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + benzoate
-
 ?
3.5.1.17 N6-carbobenzoxy-L-lysine + H2O
-
 Columba sp. L-lysine + benzoate
-
 ?
3.5.1.17 N6-chloroacetyl-L-lysine + H2O
-
 Gallus gallus L-lysine + chloroacetate
-
 ?
3.5.1.17 N6-chloroacetyl-L-lysine + H2O
-
 Rattus norvegicus L-lysine + chloroacetate
-
 ?
3.5.1.17 N6-chloroacetyl-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + chloroacetate
-
 ?
3.5.1.17 N6-chloroacetyl-L-lysine + H2O
-
 Columba sp. L-lysine + chloroacetate
-
 ?
3.5.1.17 N6-cyclohexanecarboxyl-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + cyclohexanecarboxylate
-
 ?
3.5.1.17 N6-dichloroacetyl-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + dichloroacetate
-
 ?
3.5.1.17 N6-glycyl-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + glycine
-
 ?
3.5.1.17 N6-iodoacetyl-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + iodoacetate
-
 ?
3.5.1.17 N6-isobutyryl-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + isobutyrate
-
 ?
3.5.1.17 N6-m-nitrobenzoyl-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + 3-nitrobenzoate
-
 ?
3.5.1.17 N6-metacrylyl-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + ?
-
 ?
3.5.1.17 N6-p-nitrobenzoyl-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + 4-nitrobenzoate
-
 ?
3.5.1.17 N6-phenylacetyl-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + phenylacetate
-
 ?
3.5.1.17 N6-trichloroacetyl-L-lysine + H2O
-
 Achromobacter pestifer L-lysine + trichloroacetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.17 epsilon-lysine acylase
-
 Gallus gallus
3.5.1.17 epsilon-lysine acylase
-
 Rattus norvegicus
3.5.1.17 epsilon-lysine acylase
-
 Achromobacter pestifer
3.5.1.17 epsilon-lysine acylase
-
 Columba sp.
3.5.1.17 epsilon-N-acyl-L-lysine amidohydrolase
-
 Achromobacter pestifer

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.17 4.8 5.2
-
 Achromobacter pestifer
3.5.1.17 6
-
-
 Pseudomonas sp.
3.5.1.17 7
-
-
 Rattus norvegicus
3.5.1.17 8.2 8.4
-
 Aspergillus oryzae