Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Javid-Majd, F.; Blanchard, J.S.
    Mechanistic analysis of the argE-encoded N-acetylornithine deacetylase (2000), Biochemistry, 39, 1285-1293.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.16 phosphate
-
 Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.16 PCR-amplified argE, cloned and expressed Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.16 1,10-phenanthroline zinc-specific chelator Escherichia coli
3.5.1.16 acetate product inhibition Escherichia coli
3.5.1.16 EDTA
-
 Escherichia coli
3.5.1.16 NaF linear uncompetitive inhibition Escherichia coli
3.5.1.16 ornithine product inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.16 0.81
-
 Nalpha-acetyl-L-methionine
-
 Escherichia coli
3.5.1.16 1
-
 Nalpha-acetyl-L-alanine
-
 Escherichia coli
3.5.1.16 1.3
-
 Nalpha-Acetyl-L-ornithine in presence of Co2+ or Ni2+ Escherichia coli
3.5.1.16 2
-
 N-formyl-L-methionine
-
 Escherichia coli
3.5.1.16 2.2
-
 acetylornithine in presence of Mg2+ Escherichia coli
3.5.1.16 2.4
-
 Nalpha-acetyl-L-glutamine
-
 Escherichia coli
3.5.1.16 2.5
-
 Nalpha-Acetyl-L-ornithine
-
 Escherichia coli
3.5.1.16 3.8
-
 Nalpha-Acetyl-L-ornithine in presence of Cd2+ Escherichia coli
3.5.1.16 4.1
-
 Nalpha-acetyl-L-lysine
-
 Escherichia coli
3.5.1.16 6.7
-
 Nalpha-acetyl-DL-serine
-
 Escherichia coli
3.5.1.16 7
-
 Nalpha-acetyl-L-leucine
-
 Escherichia coli
3.5.1.16 7
-
 Nalpha-chloroacetylglycine
-
 Escherichia coli
3.5.1.16 7.2
-
 Nalpha-Acetyl-L-ornithine in presence of Zn2+ Escherichia coli
3.5.1.16 8.3
-
 Nalpha-acetyl-L-asparagine
-
 Escherichia coli
3.5.1.16 13
-
 Nalpha-acetyl-L-cysteine
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.16 Co2+ enzyme is a metalloprotein, activated by a Co2+ complex Escherichia coli
3.5.1.16 Co2+ activity increases 8fold Escherichia coli
3.5.1.16 PO43- activation Escherichia coli
3.5.1.16 Zn2+ 0.1 mM activity increases 2-fold, further increase in concentration results in inhibition Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.16 42350
-
 deduced from nucleotide sequence of argE gene Escherichia coli
3.5.1.16 42350
-
 electrospray ionization mass spectrometry Escherichia coli
3.5.1.16 43000
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.16 N2-acety-L-ornithine + H2O Escherichia coli step in arginine biosynthesis L-ornithine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.16 Escherichia coli
-
 K-12
-
3.5.1.16 Escherichia coli
-
 160-37
-
3.5.1.16 Escherichia coli
-
 ATCC9637
-
3.5.1.16 Escherichia coli 160-37
-
 160-37
-
3.5.1.16 Escherichia coli ATCC 9637
-
 ATCC9637
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.16 purification of recombinant enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.16 N-acetyl-DL-methionine + H2O
-
 Escherichia coli methionine + acetate
-
 ?
3.5.1.16 N-acetyl-DL-methionine + H2O
-
 Escherichia coli 160-37 methionine + acetate
-
 ?
3.5.1.16 N-acetyl-DL-methionine + H2O
-
 Escherichia coli ATCC 9637 methionine + acetate
-
 ?
3.5.1.16 N-formyl-L-methionine + H2O
-
 Escherichia coli L-methionine + formate
-
 ?
3.5.1.16 N-formyl-L-methionine + H2O
-
 Escherichia coli 160-37 L-methionine + formate
-
 ?
3.5.1.16 N-formyl-L-methionine + H2O
-
 Escherichia coli ATCC 9637 L-methionine + formate
-
 ?
3.5.1.16 N2-acety-L-ornithine + H2O step in arginine biosynthesis Escherichia coli L-ornithine + acetate
-
 ?
3.5.1.16 Nalpha-acetyl-DL-serine + H2O
-
 Escherichia coli DL-serine + acetate
-
 ?
3.5.1.16 Nalpha-acetyl-L-alanine + H2O
-
 Escherichia coli L-alanine + acetate
-
 ?
3.5.1.16 Nalpha-acetyl-L-alanine + H2O
-
 Escherichia coli 160-37 L-alanine + acetate
-
 ?
3.5.1.16 Nalpha-acetyl-L-alanine + H2O
-
 Escherichia coli ATCC 9637 L-alanine + acetate
-
 ?
3.5.1.16 Nalpha-acetyl-L-asparagine + H2O
-
 Escherichia coli L-asparagine + acetate
-
 ?
3.5.1.16 Nalpha-acetyl-L-cysteine + H2O
-
 Escherichia coli L-cysteine + acetate
-
 ?
3.5.1.16 Nalpha-acetyl-L-glutamine + H2O
-
 Escherichia coli L-glutamine + acetate
-
 ?
3.5.1.16 Nalpha-acetyl-L-glutamine + H2O
-
 Escherichia coli 160-37 L-glutamine + acetate
-
 ?
3.5.1.16 Nalpha-acetyl-L-glutamine + H2O
-
 Escherichia coli ATCC 9637 L-glutamine + acetate
-
 ?
3.5.1.16 Nalpha-acetyl-L-leucine + H2O
-
 Escherichia coli L-leucine + acetate
-
 ?
3.5.1.16 Nalpha-acetyl-L-lysine + H2O
-
 Escherichia coli L-lysine + acetate
-
 ?
3.5.1.16 Nalpha-acetyl-L-methionine + H2O
-
 Escherichia coli acetate + L-methionine
-
 ?
3.5.1.16 Nalpha-acetyl-L-ornithine + H2O
-
 Escherichia coli L-ornithine + acetate
-
 ?
3.5.1.16 Nalpha-acetyl-L-ornithine + H2O
-
 Escherichia coli 160-37 L-ornithine + acetate
-
 ?
3.5.1.16 Nalpha-acetyl-L-ornithine + H2O
-
 Escherichia coli ATCC 9637 L-ornithine + acetate
-
 ?
3.5.1.16 Nalpha-chloroacetylglycine + H2O
-
 Escherichia coli glycine + chloroacetate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.16 7
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.16 5.3 8.7
-
 Escherichia coli