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Literature summary extracted from
- Biotinidase (1990), Methods Enzymol., 184, 103-111.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.5.1.12 | medicine | plasma biotinidase activity assay for diagnosis and screen newborn infants for biotinidase deficiency, treatment of inborn metabolism errors with large doses of biotin | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.12 | biotin | competitive inhibition at acidic pH, no inhibition at ph 7.4 with p-(N-biotinylamino)benzoic acid as substrate | Enterococcus faecalis |  |
| 3.5.1.12 | biotin | competitive inhibition at acidic pH, no inhibition at ph 7.4 with p-(N-biotinylamino)benzoic acid as substrate | Homo sapiens | |
| 3.5.1.12 | biotin | competitive inhibition at acidic pH, no inhibition at ph 7.4 with p-(N-biotinylamino)benzoic acid as substrate | Lacticaseibacillus casei | |
| 3.5.1.12 | biotin | competitive inhibition at acidic pH, no inhibition at ph 7.4 with p-(N-biotinylamino)benzoic acid as substrate | Sus scrofa | |
| 3.5.1.12 | CuCl2 | - |
Homo sapiens | |
| 3.5.1.12 | CuCl2 | - |
Rattus rattus | |
| 3.5.1.12 | diisopropylfluorophosphate | - |
Lacticaseibacillus casei | |
| 3.5.1.12 | diisopropylfluorophosphate | - |
Rattus rattus | |
| 3.5.1.12 | diisopropylfluorophosphate | - |
Sus scrofa | |
| 3.5.1.12 | monoiodoacetate | - |
Homo sapiens | |
| 3.5.1.12 | monoiodoacetate | - |
Sus scrofa | |
| 3.5.1.12 | p-hydroxymercuribenzoate | not inhibited | Enterococcus faecalis | |
| 3.5.1.12 | p-hydroxymercuribenzoate | - |
Homo sapiens | |
| 3.5.1.12 | p-hydroxymercuribenzoate | not inhibited | Lacticaseibacillus casei | |
| 3.5.1.12 | p-hydroxymercuribenzoate | - |
Rattus rattus | |
| 3.5.1.12 | p-hydroxymercuribenzoate | - |
Sus scrofa | |
| 3.5.1.12 | phenylmethylsulfonyl fluoride | - |
Homo sapiens | |
| 3.5.1.12 | ZnCl2 | - |
Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.12 | 0.005 | - |
epsilon-N-biotinyl-L-lysine | - |
Homo sapiens | |
| 3.5.1.12 | 0.0062 | - |
epsilon-N-biotinyl-L-lysine | human plasma | Homo sapiens | |
| 3.5.1.12 | 0.0077 | - |
epsilon-N-biotinyl-L-lysine | - |
Homo sapiens | |
| 3.5.1.12 | 0.0078 | - |
epsilon-N-biotinyl-L-lysine | - |
Homo sapiens | |
| 3.5.1.12 | 0.008 | - |
epsilon-N-biotinyl-L-lysine | serum | Sus scrofa | |
| 3.5.1.12 | 0.0306 | - |
p-(N-biotinylamino)benzoic acid | - |
Homo sapiens | |
| 3.5.1.12 | 0.0319 | - |
p-(N-biotinylamino)benzoic acid | - |
Homo sapiens | |
| 3.5.1.12 | 0.0333 | - |
p-(N-biotinylamino)benzoic acid | - |
Homo sapiens | |
| 3.5.1.12 | 0.0341 | - |
p-(N-biotinylamino)benzoic acid | - |
Homo sapiens | |
| 3.5.1.12 | 0.055 | - |
biotinyl-4-aminobenzoic acid | porcine serum | Sus scrofa | |
| 3.5.1.12 | 0.1002 | - |
m-(N-biotinylamino)benzoic acid | - |
Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.1.12 | microsome | rough endoplasmatic reticulum and Golgi apparatus | Rattus rattus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.12 | 66000 | 76000 | SDS-PAGE, sedimentation analysis | Homo sapiens |
| 3.5.1.12 | 76000 | - |
- |
Sus scrofa |
| 3.5.1.12 | 76000 | - |
gel filtration | Homo sapiens |
| 3.5.1.12 | 110000 | - |
- |
Homo sapiens |
| 3.5.1.12 | 115000 | - |
- |
Homo sapiens |
| 3.5.1.12 | 115000 | - |
gel filtration | Sus scrofa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.12 | biotin amide + H2O | Enterococcus faecalis | - |
? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | Lacticaseibacillus casei | - |
? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | Rattus rattus | - |
? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | Homo sapiens | cleaves D-biotinylamides and esters, recycling of the vitamin | ? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | Sus scrofa | cleaves D-biotinylamides and esters, recycling of the vitamin | ? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | Homo sapiens | - |
? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | Sus scrofa | - |
? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | Enterococcus faecalis | - |
? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | Lacticaseibacillus casei | - |
? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | Rattus rattus | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.12 | Enterococcus faecalis | - |
- |
- |
| 3.5.1.12 | Homo sapiens | - |
human | - |
| 3.5.1.12 | Lacticaseibacillus casei | - |
- |
- |
| 3.5.1.12 | Rattus rattus | - |
rat | - |
| 3.5.1.12 | Sus scrofa | - |
pig | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.12 | - |
Enterococcus faecalis |
| 3.5.1.12 | - |
Lacticaseibacillus casei |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.1.12 | adrenal gland | - |
Homo sapiens | - |
| 3.5.1.12 | blood | serum, plasma, leukocytes | Homo sapiens | - |
| 3.5.1.12 | blood | serum, plasma, leukocytes | Sus scrofa | - |
| 3.5.1.12 | blood | serum, plasma, leukocytes | Rattus rattus | - |
| 3.5.1.12 | fibroblast | - |
Homo sapiens | - |
| 3.5.1.12 | kidney | - |
Homo sapiens | - |
| 3.5.1.12 | kidney | - |
Sus scrofa | - |
| 3.5.1.12 | kidney | - |
Rattus rattus | - |
| 3.5.1.12 | liver | - |
Homo sapiens | - |
| 3.5.1.12 | liver | - |
Sus scrofa | - |
| 3.5.1.12 | liver | - |
Rattus rattus | - |
| 3.5.1.12 | small intestine | - |
Homo sapiens | - |
| 3.5.1.12 | small intestine | - |
Rattus rattus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.1.12 | -70°C stable | Homo sapiens |
| 3.5.1.12 | 4°C, 0.1 M phosphate buffer pH 6.0, 1 mM 2-mercaptoethanol, 1 mM EDTA, 1 month without loss of activity | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.12 | biotin amide + H2O | - |
Enterococcus faecalis | ? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | - |
Lacticaseibacillus casei | ? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | - |
Rattus rattus | ? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | cleaves D-biotinylamides and esters, recycling of the vitamin | Homo sapiens | ? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | cleaves D-biotinylamides and esters, recycling of the vitamin | Sus scrofa | ? | - |
? | |
| 3.5.1.12 | biotinyl-4-aminobenzoic acid + H2O | - |
Homo sapiens | biotin + 4-aminobenzoic acid | - |
? | |
| 3.5.1.12 | biotinyl-4-aminobenzoic acid + H2O | - |
Sus scrofa | biotin + 4-aminobenzoic acid | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Sus scrofa | biotin + L-lysine | - |
ir | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Enterococcus faecalis | biotin + L-lysine | - |
ir | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Lacticaseibacillus casei | biotin + L-lysine | - |
ir | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Rattus rattus | biotin + L-lysine | - |
ir | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | cannot cleave biocytin that is bound to avidin | Homo sapiens | biotin + L-lysine | - |
ir | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Sus scrofa | ? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Enterococcus faecalis | ? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Lacticaseibacillus casei | ? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Rattus rattus | ? | - |
? | |
| 3.5.1.12 | N-(+)biotinyl-4-aminobenzoic acid + H2O | - |
Homo sapiens | 4-aminobenzoic acid + biotin | - |
? | |
| 3.5.1.12 | N-biotinyl-3-aminobenzoic acid + H2O | - |
Homo sapiens | biotin + 3-aminobenzoic acid | - |
? | |
| 3.5.1.12 | N-biotinyl-3-aminobenzoic acid + H2O | - |
Sus scrofa | biotin + 3-aminobenzoic acid | - |
? | |
| 3.5.1.12 | N-biotinyl-beta-alanine + H2O | - |
Sus scrofa | biotin + beta-alanine | - |
? | |
| 3.5.1.12 | N-biotinyl-beta-alanine + H2O | - |
Enterococcus faecalis | biotin + beta-alanine | - |
? | |
| 3.5.1.12 | N-biotinyl-beta-alanine + H2O | - |
Lacticaseibacillus casei | biotin + beta-alanine | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.12 | monomer | - |
Homo sapiens |
| 3.5.1.12 | monomer | - |
Sus scrofa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.12 | 60 | 70 | denaturation after 30 min at 60°C | Sus scrofa |
| 3.5.1.12 | 60 | 70 | 15 min 60°C 60% activity lost, 70°C activity completely lost | Homo sapiens |
| 3.5.1.12 | 60 | 70 | stable up to 60°C, stable 30 min at 60°C, at 70°C activity decreases after 10 min to 20-50% of the initial value | Rattus rattus |
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