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Literature summary extracted from

  • Duerre, J.A.
    S-Adenosylhomocysteine nucleosidase (Escherichia coli) (1971), Methods Enzymol., 17, 411-415.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.2.9 unstable in solution of low ionic strength Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.9 5.4
-
 5'-methylthioadenosine
-
 Escherichia coli
3.2.2.9 9
-
 S-adenosylhomocysteine
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.9 5'-methylthioadenosine + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
 ?
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O Escherichia coli
-
 ?
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O Klebsiella aerogenes
-
 ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
 ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O Escherichia coli
-
 ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O Klebsiella aerogenes
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.9 Escherichia coli
-
-
-
3.2.2.9 Klebsiella aerogenes
-
-
-
3.2.2.9 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.9
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.9 3.6
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.2.2.9 0°C, 0.1 M phosphate buffer containing 0.009 M 2-mercaptoethanol and 0.0005 M EDTA stable for 2 weeks, retains 80% of its total activity after 2 weeks at -40°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.9 5'-methylthioadenosine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium 5-methylthio-D-ribose + adenine
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Escherichia coli 5-methylthio-D-ribose + adenine
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Klebsiella aerogenes 5-methylthio-D-ribose + adenine
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Escherichia coli ?
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Klebsiella aerogenes ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium adenine + S-ribosyl-L-homocysteine
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Escherichia coli adenine + S-ribosyl-L-homocysteine
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Klebsiella aerogenes adenine + S-ribosyl-L-homocysteine
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Escherichia coli ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Klebsiella aerogenes ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.9 6.5
-
 optimal pH for both natural substrates, 5'-methylthioadenosine and S-adenosyl-L-homocysteine, with little variation from pH 5.7-7.1 Escherichia coli