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Literature summary extracted from

  • Guranowski, A.B.; Chiang, P.K.; Cantoni, G.L.
    5'-Methylthioadenosine nucleosidase. Purification and characterization of the enzyme from Lupinus luteus seeds (1981), Eur. J. Biochem., 114, 293-299.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.16 3-Deazaadenine
-
 Lupinus luteus
3.2.2.16 9-erythro-(2-hydroxyl 3-nonyl)adenine
-
 Lupinus luteus
3.2.2.16 adenine potent competitive inhibitor, feedback inhibition by one of its products Lupinus luteus
3.2.2.16 Adenosine 5'-carboxamide
-
 Lupinus luteus
3.2.2.16 Decoyinine
-
 Lupinus luteus
3.2.2.16 sinefungin
-
 Lupinus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.16 0.00031
-
 5'-deoxy-5'-methylthioadenosine
-
 Escherichia coli
3.2.2.16 0.00041
-
 5'-deoxy-5'-methylthioadenosine
-
 Lupinus luteus
3.2.2.16 0.055
-
 5'-methylthioinosine
-
 Lupinus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.16 31000
-
-
 Escherichia coli
3.2.2.16 31000
-
 2 * 31000, SDS-PAGE Lupinus luteus
3.2.2.16 62000
-
 gel filtration Lupinus luteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.16 5'-methylthioadenosine + H2O Escherichia coli
-
 ?
-
 ?
3.2.2.16 5'-methylthioadenosine + H2O Lupinus luteus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.16 Escherichia coli
-
-
-
3.2.2.16 Lupinus luteus
-
 yellow lupin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.16
-
 Lupinus luteus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.16 seed
-
 Lupinus luteus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.16 27
-
-
 Lupinus luteus

Storage Stability

EC Number Storage Stability Organism
3.2.2.16 -20°C, 50 mM Bicine buffer, 5% glycerol buffer, stable for at least 3 months, tolerates repeated freezing and thawing Lupinus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.16 5'-deoxy-5'-isobutylthio-adenosine + H2O 1-butanol buffer/propan-2-ol buffer Lupinus luteus 5-isobutylthio-D-ribose + adenine
-
 ?
3.2.2.16 5'-deoxy-5'-propylthioadenosine + H2O 1-butanol buffer/propan-2-ol buffer Lupinus luteus 5-propylthio-D-ribose + adenine
-
 ?
3.2.2.16 5'-deoxyadenosine + H2O 1-butanol buffer Lupinus luteus 5-deoxy-D-ribose + adenine
-
 ?
3.2.2.16 5'-ethylthio-2-aminoadenosine + H2O 1-butanol buffer/propan-2-ol buffer Lupinus luteus 5-ethylthio-D-ribose + 2-aminoadenosine
-
 ?
3.2.2.16 5'-ethylthioadenosine + H2O 1-butanol buffer Lupinus luteus 5-ethylthio-D-ribose + adenine
-
 ?
3.2.2.16 5'-ethylthioinosine + H2O 1-butanol buffer Lupinus luteus 5-ethylthio-D-ribose + hypoxanthine
-
 ?
3.2.2.16 5'-isobutylthio-3-deaza-adenosine + H2O 1-butanol buffer/propan-2-ol buffer Lupinus luteus 5-isobutylthio-D-ribose + 3-deazadenosine
-
 ?
3.2.2.16 5'-isobutylthioinosine + H2O 1-butanol buffer Lupinus luteus 5-isobutylthio-D-ribose + hypoxanthine
-
 ?
3.2.2.16 5'-methylthioadenosine + H2O
-
 Escherichia coli ?
-
 ?
3.2.2.16 5'-methylthioadenosine + H2O
-
 Lupinus luteus ?
-
 ?
3.2.2.16 5'-methylthioarabinosyladenine + H2O 1-butanol buffer/propan-2-ol buffer Lupinus luteus 5-methylthioarabinose + adenine
-
 ?
3.2.2.16 5'-methylthioinosine + H2O maximal velocity about 50-times lower than for 5'-methylthioadenosine Lupinus luteus 5-methylthio-D-ribose + hypoxanthine
-
 ?
3.2.2.16 5'-n-butylthioadenosine + H2O 1-butanol buffer Lupinus luteus 5-n-butylthio-D-ribose + adenine
-
 ?
3.2.2.16 5'-n-butylthioinosine + H2O 1-butanol buffer Lupinus luteus 5-n-butylthio-D-ribose + hypoxanthine
-
 ?
3.2.2.16 S-adenosylmercaptoethanol + H2O 1-butanol buffer/propan-2-ol buffer Lupinus luteus ?
-
 ?
3.2.2.16 S-inosylmercaptoethanol + H2O propan-2-ol buffer Lupinus luteus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.16 dimer 2 * 31000, SDS-PAGE Lupinus luteus

Synonyms

EC Number Synonyms Comment Organism
3.2.2.16 lupin MeSAdo nucleosidase
-
 Lupinus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.16 6.8
-
 50 mM potassium phosphate buffer Escherichia coli
3.2.2.16 8 8.5
-
 Lupinus luteus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.2.16 6.5 9.5
-
 Lupinus luteus