EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.2.14 | ATP | less effective activator than GTP | Azotobacter vinelandii | |
3.2.2.14 | dGMP | - |
Azotobacter vinelandii | |
3.2.2.14 | dGTP | - |
Azotobacter vinelandii | |
3.2.2.14 | GDP | - |
Azotobacter vinelandii | |
3.2.2.14 | GMP | - |
Azotobacter vinelandii | |
3.2.2.14 | GTP | most potent activator | Azotobacter vinelandii | |
3.2.2.14 | guanosine 2'-monophosphate | - |
Azotobacter vinelandii | |
3.2.2.14 | guanosine 3'-monophosphate | - |
Azotobacter vinelandii | |
3.2.2.14 | guanosine 5'-tetraphosphate | most potent activator | Azotobacter vinelandii |
EC Number | General Stability | Organism |
---|---|---|
3.2.2.14 | remains active for 1 week at 4°C in 0.025 M Tris-HCl, pH 7.5, containing 1 mM reduced glutathione | Azotobacter vinelandii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.2.14 | Cd2+ | 1 mM | Azotobacter vinelandii | |
3.2.2.14 | Cu2+ | 1 mM | Azotobacter vinelandii | |
3.2.2.14 | dCMP | effective inhibition in presence of 1 mM GTP, noncompetitive inhibitor | Azotobacter vinelandii | |
3.2.2.14 | dGMP | effective inhibition in presence of 1 mM GTP, noncompetitive inhibitor | Azotobacter vinelandii | |
3.2.2.14 | EDTA | 1 mM inhibits NMN hydrolysis by 30% | Azotobacter vinelandii | |
3.2.2.14 | GMP | effective inhibition in presence of 1 mM GTP | Azotobacter vinelandii | |
3.2.2.14 | N-ethylmaleimide | 50% inhibition at 80 mM | Azotobacter vinelandii | |
3.2.2.14 | p-chloromercuribenzoate | 50% inhibition at 3 mM | Azotobacter vinelandii | |
3.2.2.14 | Zn2+ | 1 mM | Azotobacter vinelandii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.14 | 2 | - |
nicotinamide mononucleotide | in absence of GTP | Azotobacter vinelandii | |
3.2.2.14 | 4 | - |
nicotinamide mononucleotide | 0.52 mM dGTP as effector | Azotobacter vinelandii | |
3.2.2.14 | 4.4 | - |
nicotinamide mononucleotide | 0.5 mM guanosine 5'-tetraphosphate as effector | Azotobacter vinelandii | |
3.2.2.14 | 4.5 | - |
nicotinamide mononucleotide | 0.5 mM/1 mM GTP | Azotobacter vinelandii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.2.14 | 213000 | - |
gel filtration, Sephadex G-200 | Azotobacter vinelandii |
3.2.2.14 | 240000 | - |
gel filtration, Sepharose 6B | Azotobacter vinelandii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.14 | nicotinamide mononucleotide + H2O | Escherichia coli | conversion to nicotinamide as a precursor of NAD+ via nicotinic acid | ? | - |
? | |
3.2.2.14 | nicotinamide mononucleotide + H2O | Azotobacter vinelandii | conversion to nicotinamide as a precursor of NAD+ via nicotinic acid | ? | - |
? | |
3.2.2.14 | nicotinamide mononucleotide + H2O | Azotobacter vinelandii O | conversion to nicotinamide as a precursor of NAD+ via nicotinic acid | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.2.14 | Azotobacter vinelandii | - |
- |
- |
3.2.2.14 | Azotobacter vinelandii O | - |
- |
- |
3.2.2.14 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.2.14 | partially from sonic extract, purified enzyme loses over 90% of its activity after Sephadex G-200 treatment | Azotobacter vinelandii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.2.14 | 0.0051 | - |
- |
Escherichia coli |
3.2.2.14 | 0.0069 | - |
- |
Escherichia coli |
3.2.2.14 | 0.0195 | - |
- |
Azotobacter vinelandii |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.2.14 | 0 to -16°C stored for a month without significant loss of activity | Azotobacter vinelandii |
3.2.2.14 | 0°C, 0.025 M Tris-HCl, pH 9, containing 1 mM reduced glutathione, one-half of enzyme activity lost in 5 days | Azotobacter vinelandii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.14 | nicotinamide mononucleotide + H2O | - |
Escherichia coli | nicotinamide + ribose 5-phosphate | - |
? | |
3.2.2.14 | nicotinamide mononucleotide + H2O | irreversible hydrolysis of the N-ribosidic linkage of NMN | Azotobacter vinelandii | nicotinamide + ribose 5-phosphate | - |
ir | |
3.2.2.14 | nicotinamide mononucleotide + H2O | irreversible hydrolysis of the N-ribosidic linkage of NMN | Azotobacter vinelandii O | nicotinamide + ribose 5-phosphate | - |
ir | |
3.2.2.14 | nicotinamide mononucleotide + H2O | conversion to nicotinamide as a precursor of NAD+ via nicotinic acid | Escherichia coli | ? | - |
? | |
3.2.2.14 | nicotinamide mononucleotide + H2O | conversion to nicotinamide as a precursor of NAD+ via nicotinic acid | Azotobacter vinelandii | ? | - |
? | |
3.2.2.14 | nicotinamide mononucleotide + H2O | conversion to nicotinamide as a precursor of NAD+ via nicotinic acid | Azotobacter vinelandii O | ? | - |
? | |
3.2.2.14 | nicotinic acid mononucleotide + H2O | NaNM reaction slowly, 8% hydrolysis of the rate of NMN | Azotobacter vinelandii | nicotinic acid + ribose 5-phosphate | - |
? | |
3.2.2.14 | nicotinic acid mononucleotide + H2O | NaNM reaction slowly, 8% hydrolysis of the rate of NMN | Azotobacter vinelandii O | nicotinic acid + ribose 5-phosphate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.2.14 | 39 | - |
- |
Azotobacter vinelandii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.2.14 | 8.5 | 9 | - |
Azotobacter vinelandii |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.14 | 3 | - |
p-chloromercuribenzoate | - |
Azotobacter vinelandii | |
3.2.2.14 | 80 | - |
N-ethylmaleimide | - |
Azotobacter vinelandii |