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Literature summary extracted from

  • McKie, V.A.; Black, G.W.; Millward-Sadler, S.J.; Hazlewood, G.P.; Laurie, J.I.; Gilbert, H.J.
    Arabinanase A from Pseudomonas fluorescens subsp. cellulosa exhibits both an endo- and an exo-mode of action (1997), Biochem. J., 323, 547-555.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.99 expressed in Escherichia coli Pseudomonas fluorescens
3.2.1.99 hyperexpression in Escherichia coli Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.99 additional information
-
 additional information
-
 Pseudomonas fluorescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.99 extracellular
-
 Pseudomonas fluorescens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.99 34000
-
 SDS-PAGE Pseudomonas fluorescens
3.2.1.99 39440
-
 calculated from nucleotide sequence Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.99 Pseudomonas fluorescens
-
 arabinanase A, exhibits endo- and exo-mode of action
-
3.2.1.99 Pseudomonas fluorescens
-
 subspecies cellulosa
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.99 recombinant arabinanase Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.99 additional information
-
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.99 1,5-alpha-L-arabinan + H2O linear arabinan Pseudomonas fluorescens alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + ? main product ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O poor substrate Pseudomonas fluorescens alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
 ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
 Pseudomonas fluorescens alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranose
-
 ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
 Pseudomonas fluorescens alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
 ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
 Pseudomonas fluorescens alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
 ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
 Pseudomonas fluorescens alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
 ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
 Pseudomonas fluorescens alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
 ?
3.2.1.99 carboxymethylarabinan + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.2.1.99 L-arabinan + H2O linear arabinan Pseudomonas fluorescens L-arabino-oligosaccharides main product: L-arabinotriose ?
3.2.1.99 L-arabinan + H2O branched sugar beet arabinan containing alpha-1,3-arabinose side chains: not a substrate Pseudomonas fluorescens L-arabino-oligosaccharides main product: L-arabinotriose ?
3.2.1.99 L-arabino-oligosaccharide + H2O
-
 Pseudomonas fluorescens L-arabino-oligosaccharide main product: L-arabinotriose ?
3.2.1.99 additional information inactive on cellulose, avicel, amorphous cellulose, carboxymethylcellulose, xylan, galactan, mannan, 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl alpha-L-arabinopyranoside, 4-nitrophenyl beta-xyloside and 2,4-dinitrophenyl beta-xylobioside Pseudomonas fluorescens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.99 arabinanase A exhibits endo- and exo-mode of action Pseudomonas fluorescens
3.2.1.99 arbA exhibits endo- and exo-mode of action Pseudomonas fluorescens