Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lerouge, P.; O'Neill, M.A.; Darvill, A.G.; Albersheim, P.
    The purification of commercially available endo-alpha-L-arabinanases and alpha-L-arabinosidase for use in the structural analysis of pectic polysaccharides (1993), Carbohydr. Res., 243, 373-378.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.55 analysis commercially available enzyme, potential for use in the structural analysis of complex carbohydrates Aspergillus niger
3.2.1.99 analysis structural analysis of plant cell pectic polysaccharides and arabinans Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Aspergillus niger
-
-
-
3.2.1.99 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55
-
Aspergillus niger
3.2.1.99 by fast protein liquid chromatography, FPLC Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 1,5-alpha-L-arabinotriose + H2O
-
Aspergillus niger L-arabinose + arabinobiose
-
?
3.2.1.55 alpha-(1->5)-L-arabinofuranotriose + H2O
-
Aspergillus niger L-arabinose
-
?
3.2.1.55 L-arabinan + H2O
-
Aspergillus niger (1,5-alpha-L-arabinose)25 + ?
-
ir
3.2.1.55 additional information no release of arabinose from linear arabinan Aspergillus niger ?
-
?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Aspergillus niger p-nitrophenol + L-arabinose
-
?
3.2.1.99 1,5-alpha-L-arabinan + H2O
-
Aspergillus niger alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara product formation, overview: oligoarabinosides with degree of polymerization between 2 and 5 are the major products, but only trace amounts of free arabinose ?