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Literature summary extracted from
- Purification and physico-chemical properties of an endo-1,5-alpha-L-arabinanase (EC 3.2.1.99) isolated from an Aspergillus niger pectinase preparation (1994), Carbohydr. Polym., 24, 247-263.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.99 | analysis | - |
Aspergillus niger |
| 3.2.1.99 | nutrition | - |
Aspergillus niger |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.99 | Hg2+ | completely | Aspergillus niger |  |
| 3.2.1.99 | HgCl2 | completely | Aspergillus niger | |
| 3.2.1.99 | iodoacetamide | very slight inhibition | Aspergillus niger | |
| 3.2.1.99 | MgSO4 | very slight inhibition | Aspergillus niger | |
| 3.2.1.99 | additional information | no inhibition by CaCl2, EDTA, L-arabono-gamma-lactone, L-arabinose, D-arabitol | Aspergillus niger | |
| 3.2.1.99 | Zn2+ | - |
Aspergillus niger | |
| 3.2.1.99 | ZnSO4 | - |
Aspergillus niger | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.99 | additional information | - |
additional information | kinetic data | Aspergillus niger |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.99 | 33900 | - |
MALDI-TOF-MS | Aspergillus niger |
| 3.2.1.99 | 41000 | - |
gel filtration | Aspergillus niger |
| 3.2.1.99 | 42500 | - |
SDS-PAGE | Aspergillus niger |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.99 | Aspergillus niger | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.99 | glycoprotein | - |
Aspergillus niger |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.99 | - |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.99 | 14 | - |
substrate: branched sugar beet arabinan | Aspergillus niger |
| 3.2.1.99 | 81.1 | - |
substrate: debranched sugar beet arabinan | Aspergillus niger |
| 3.2.1.99 | 90.2 | - |
best substrate: linear 1,5-alpha-L-arabinan | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.99 | 1,5-alpha-L-arabinan + H2O | best substrate, endo mechanism | Aspergillus niger | alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara | - |
? | |
| 3.2.1.99 | 1,5-alpha-L-arabinan + H2O | best substrate: linear 1,5-alpha-L-haze arabinan | Aspergillus niger | alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara | - |
? | |
| 3.2.1.99 | galactan + H2O | very poor substrate | Aspergillus niger | ? | - |
? | |
| 3.2.1.99 | L-arabinan + H2O | debranched and branched sugar beet arabinan | Aspergillus niger | L-arabino-oligosaccharides | - |
? | |
| 3.2.1.99 | additional information | substrate specificity, overview | Aspergillus niger | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.99 | endo-ABA | - |
Aspergillus niger |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.99 | 55 | - |
- |
Aspergillus niger |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.99 | 40 | - |
long incubation times, 26.5 h, at 40°C: 90% activity retained | Aspergillus niger |
| 3.2.1.99 | 45 | - |
up to 45°C: more than 95% activity retained for 3 h | Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.99 | 4.5 | 5 | - |
Aspergillus niger |
| 3.2.1.99 | 4.8 | - |
maximum | Aspergillus niger |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.99 | 3.5 | 8 | 85% activity retained between, 67 h at 25°C | Aspergillus niger |
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