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Literature summary extracted from

  • Dunkel, M.P.H.; Amado, R.
    Analysis of endo-(1->5)-alpha-L-arabinanase degradation patterns of linear (1->5)-alpha-L-arabino-oligosaccharides by high-performance anion-exchange chromatography with pulsed amperometric detection (1995), Carbohydr. Res., 268, 151-158.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.99 analysis
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Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.99 Aspergillus niger
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-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.99
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Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O mild hydrolysis: no significant degradation, exhaustive enzymatic hydrolysis: extremely low degradation Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP3 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP4 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP4 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose product formation ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP4 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose intermediate enzymatic hydrolysis: L-arabinobiose and much less L-arabinose + L-arabinotriose ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP4 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose end-products after exhaustive enzymatic degradation ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP5 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP5 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose product formation ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP5 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose end-products after exhaustive enzymatic degradation ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP5 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose intermediate enzymatic hydrolysis: L-arabinobiose + L-arabinotriose ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP6 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP6 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose product formation ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP6 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose end-products after exhaustive enzymatic degradation ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP6 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose intermediate enzymatic hydrolysis: L-arabinobiose + L-arabinotetraose and L-arabinotriose ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP7 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP7 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose product formation ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP7 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose end-products after exhaustive enzymatic degradation ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP7 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose intermediate enzymatic hydrolysis: L-arabinotriose + L-arabinotetraose ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP8 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP8 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose product formation ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP8 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose end-products after exhaustive enzymatic degradation ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O DP8 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose intermediate enzymatic hydrolysis: L-arabinotriose + L-arabinopentaose and L-arabinobiose + L-arabinohexaose and L-arabinotetraose ?
3.2.1.99 alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + H2O no or extremely low degradation Aspergillus niger L-arabinose
-
?
3.2.1.99 alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + H2O DP2 Aspergillus niger L-arabinose
-
?
3.2.1.99 L-arabino-oligosaccharide + H2O degree of polymerization 2-8, DP2-8 Aspergillus niger L-arabino-oligosaccharide composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times ?
3.2.1.99 L-arabino-oligosaccharide + H2O degree of polymerization 2-8, DP2-8 Aspergillus niger L-arabino-oligosaccharide L-arabinose + L-arabinobiose: end-products after exhaustive enzymatic degradation ?
3.2.1.99 L-arabino-oligosaccharide + H2O degree of polymerization 2-8, DP2-8 Aspergillus niger L-arabino-oligosaccharide product formation ?
3.2.1.99 L-arabino-oligosaccharide + H2O linear 1,5-alpha-L-arabino-oligosaccharide Aspergillus niger L-arabino-oligosaccharide composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times ?
3.2.1.99 L-arabino-oligosaccharide + H2O linear 1,5-alpha-L-arabino-oligosaccharide Aspergillus niger L-arabino-oligosaccharide L-arabinose + L-arabinobiose: end-products after exhaustive enzymatic degradation ?
3.2.1.99 L-arabino-oligosaccharide + H2O linear 1,5-alpha-L-arabino-oligosaccharide Aspergillus niger L-arabino-oligosaccharide product formation ?
3.2.1.99 additional information substrate specificity, overview Aspergillus niger ?
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?
3.2.1.99 additional information product specificity, overview Aspergillus niger ?
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?
3.2.1.99 additional information composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times Aspergillus niger ?
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?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.99 endo-ABA
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Aspergillus niger