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Literature summary extracted from

  • Pitson, S.M.; Voragen, A.G.J.; Vincken, J.P.; Beldman, G.
    Action patterns and mapping of the substrate-binding regions of endo-(1->5)-alpha-L-arabinanases from Aspergillus niger and Aspergillus aculeatus (1997), Carbohydr. Res., 303, 207-218.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.99 nutrition commercial applications in fruit and vegetable processing, particularly in apple juice manufacture where their use prevents haze formation in pectinase-treated apple pulp by depolymerizing debranched arabinan, linear alpha-1,5-arabinan, formed by the action of alpha-L-arabinofuranosidases during this process Aspergillus niger
3.2.1.99 nutrition commercial applications in fruit and vegetable processing, particularly in apple juice manufacture where their use prevents haze formation in pectinase-treated apple pulp by depolymerizing debranched arabinan, linear alpha-1,5-arabinan, formed by the action of alpha-L-arabinofuranosidases during this process Aspergillus aculeatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.99 additional information
-
 additional information kinetic data and mechanism Aspergillus niger
3.2.1.99 additional information
-
 additional information kinetic data and mechanism Aspergillus aculeatus
3.2.1.99 0.388
-
 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
 Aspergillus niger
3.2.1.99 0.592
-
 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
 Aspergillus aculeatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.99 Aspergillus aculeatus
-
-
-
3.2.1.99 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.99
-
 Aspergillus niger
3.2.1.99
-
 Aspergillus aculeatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.99 1,5-alpha-L-arabinan + H2O
-
 Aspergillus niger alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara major final end-products, upon exhaustive degradation of substrate some monomers, L-arabinose, are produced ?
3.2.1.99 1,5-alpha-L-arabinan + H2O
-
 Aspergillus aculeatus alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara major final end-products, upon exhaustive degradation of substrate some monomers, L-arabinose, are produced ?
3.2.1.99 1,5-alpha-L-arabinan + H2O
-
 Aspergillus niger alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara action patterns of initial hydrolysis products ?
3.2.1.99 1,5-alpha-L-arabinan + H2O
-
 Aspergillus aculeatus alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara action patterns of initial hydrolysis products ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
 Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
 ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
 Aspergillus aculeatus alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranose
-
 ?
3.2.1.99 alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + H2O at very low rate, with high enzyme concentration Aspergillus niger L-arabinose
-
 ?
3.2.1.99 alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + H2O at very low rate, with high enzyme concentration Aspergillus aculeatus L-arabinose
-
 ?
3.2.1.99 L-arabinan + H2O random hydrolysis of L-arabinan Aspergillus niger L-arabino-oligosaccharides
-
 ?
3.2.1.99 L-arabinan + H2O random hydrolysis of L-arabinan Aspergillus aculeatus L-arabino-oligosaccharides
-
 ?
3.2.1.99 L-arabino-oligosaccharide + H2O
-
 Aspergillus niger L-arabino-oligosaccharide
-
 ?
3.2.1.99 L-arabino-oligosaccharide + H2O
-
 Aspergillus aculeatus L-arabino-oligosaccharide
-
 ?
3.2.1.99 additional information substrate specificity, overview Aspergillus niger ?
-
 ?
3.2.1.99 additional information substrate specificity, overview Aspergillus aculeatus ?
-
 ?
3.2.1.99 additional information substrate binding site, action pattern, subsite mapping Aspergillus niger ?
-
 ?
3.2.1.99 additional information substrate binding site, action pattern, subsite mapping Aspergillus aculeatus ?
-
 ?
3.2.1.99 additional information no bi-substrate reactions such as condensation or transglycosylation Aspergillus niger ?
-
 ?
3.2.1.99 additional information no bi-substrate reactions such as condensation or transglycosylation Aspergillus aculeatus ?
-
 ?
3.2.1.99 additional information product specificity, overview Aspergillus niger ?
-
 ?
3.2.1.99 additional information product specificity, overview Aspergillus aculeatus ?
-
 ?
3.2.1.99 additional information inactive on arabinobiitol Aspergillus niger ?
-
 ?
3.2.1.99 additional information inactive on arabinobiitol Aspergillus aculeatus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.99 ABN
-
 Aspergillus niger
3.2.1.99 ABN
-
 Aspergillus aculeatus