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Literature summary extracted from

  • Hayashi, T.; Akiba, T.; Horikoshi, K.
    Production and purification of new maltohexaose-forming amylases from alkalophilic Bacillus sp. H-167 (1988), Agric. Biol. Chem., 52, 443-448.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.98 synthesis very advantageous for obtaining pure maltohexaose Bacillus sp. H-167

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.98 59000
-
 H-I-2, SDS-PAGE Bacillus sp. H-167
3.2.1.98 73000
-
 H-I-1, SDS-PAGE Bacillus sp. H-167
3.2.1.98 80000
-
 H-II, SDS-PAGE Bacillus sp. H-167

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.98 Bacillus sp. H-167
-
 alkalophilic bacterium, 3 enzymes: H-I-1, H-I-2, H-II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.98
-
 Bacillus sp. H-167

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.98 (Glcalpha(1-4))m + H2O = (Glcalpha(1-4))m-6 + maltohexaose alpha-amylase, product is linear oligosaccharide containing alpha-1,4-glucosidic linkages Bacillus sp. H-167
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.98 culture filtrate
-
 Bacillus sp. H-167
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.98 116
-
 H-I-2 Bacillus sp. H-167
3.2.1.98 124
-
 H-II Bacillus sp. H-167
3.2.1.98 147
-
 H-I-1 Bacillus sp. H-167

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.98 amylopectin + H2O
-
 Bacillus sp. H-167 maltohexaose + ?
-
 ?
3.2.1.98 amylose + H2O
-
 Bacillus sp. H-167 maltohexaose
-
 ?
3.2.1.98 glycogen + H2O
-
 Bacillus sp. H-167 maltohexaose
-
 ?
3.2.1.98 maltohexaose + H2O
-
 Bacillus sp. H-167 maltose + maltotetraose main product ?
3.2.1.98 maltohexaose + H2O
-
 Bacillus sp. H-167 D-glucose + maltopentaose small amounts ?
3.2.1.98 maltohexaose + H2O
-
 Bacillus sp. H-167 maltotriose + ? small amounts ?
3.2.1.98 additional information products have alpha-configuration Bacillus sp. H-167 ?
-
 ?
3.2.1.98 additional information pullulan, dextran or alpha-, beta- or gamma-cyclodextrin: not a substrate Bacillus sp. H-167 ?
-
 ?
3.2.1.98 starch + H2O soluble starch Bacillus sp. H-167 maltohexaose + ? main product ?
3.2.1.98 starch + H2O soluble starch Bacillus sp. H-167 maltohexaose + ? 25-30% maltohexaose in early stage of hydrolysis, alpha-anomer product ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.98 maltohexaose-forming amylase
-
 Bacillus sp. H-167