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Literature summary extracted from

  • Takegawa, K.; Naitoh, T.; Iwahara, S.
    Isolation of two endo-beta-N-acetylglucosaminidases with different specificities from Pseudomonas sp. (1991), Biochem. Int., 24, 793-799.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.96 analysis the enzyme will be an excellent tool for clarification of the structures and functions of complex oligosaccharides in glycoproteins Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.96 PCMB 1 mM, 84% inhibition Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.96 94000
-
 x * 94000, endo-beta-N-acetylglucosaminidase PI, SDS-PAGE Pseudomonas sp.
3.2.1.96 106000
-
 x * 106000, endo-beta-N-acetylglucosaminidase PI, SDS-PAGE Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.96 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.96 endo-beta-N-acetylglucosaminidase PI and PII Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.96 culture medium
-
 Pseudomonas sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.96 (Man)3(GlcNAc)2-N-Asn-dansyl + H2O endo-beta-N-acetylglucosaminidase PII, no activity with endo-beta-N-acetylglucosaminidase PII Pseudomonas sp. ?
-
 ?
3.2.1.96 additional information endo-beta-N-acetylglucosaminidase PI hydrolyzes high-mannose type oligosaccharides GP-IV and GP-V faster than the hybrid type FP-I, GP-II, and GP-III. Endo-beta-N-acetylglucosaminidase PII hydrolyzes high mannose and hybrid type oligosaccharides Pseudomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.96 ? x * 106000, endo-beta-N-acetylglucosaminidase PI, SDS-PAGE Pseudomonas sp.
3.2.1.96 ? x * 94000, endo-beta-N-acetylglucosaminidase PI, SDS-PAGE Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.96 45
-
 10 min, 5% loss of activity, endo-beta-N-acetylglucosaminidase PII Pseudomonas sp.
3.2.1.96 50
-
 10 min, 60% loss of activity, endo-beta-N-acetylglucosaminidase PII Pseudomonas sp.
3.2.1.96 55
-
 10 min, stable, endo-beta-N-acetylglucosaminidase I Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.96 5 6 ENGase PI Pseudomonas sp.
3.2.1.96 6 8 ENGase PII Pseudomonas sp.