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Literature summary extracted from

  • Trimble, R.B.; Tarentino, A.L.; Evans, G.; Maley, F.
    Purification and properties of endo-beta-N-acetylglucosaminidase L from Streptomyces plicatus (1979), J. Biol. Chem., 254, 9708-9713.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.96 20-30% loss of initial activity is lost when the protein concentration is decreased to less than 0.02-0.03 mg/ml Streptomyces plicatus
3.2.1.96 stable to lyophilization Streptomyces plicatus
3.2.1.96 stable to repeated freezing and thawing Streptomyces plicatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.96 SDS 0.2%, complete inactivation Streptomyces plicatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.96 0.7
-
 Man(GlcNAc)2Asn-dansyl
-
 Streptomyces plicatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.96 46000
-
 gel filtration Streptomyces plicatus
3.2.1.96 49500
-
 equilibrium sedimentation Streptomyces plicatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.96 Streptomyces plicatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.96
-
 Streptomyces plicatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.96 additional information
-
-
 Streptomyces plicatus

Storage Stability

EC Number Storage Stability Organism
3.2.1.96 4°C, stable for up to 6 months Streptomyces plicatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.96 beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-Asn
-
 Streptomyces plicatus beta-D-Man-(1->4)-beta-D-GlcNAc + beta-D-GlcNAc-Asn
-
 ?
3.2.1.96 Man(GlcNAc)2Asn-dansyl + H2O
-
 Streptomyces plicatus ?
-
 ?
3.2.1.96 N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Streptomyces plicatus 2 N,N'-diacetylchitobiose
-
 ?
3.2.1.96 N,N',N''-triacetylchitotriose + H2O
-
 Streptomyces plicatus N-acetyl-D-glucosamine + N,N'-diacetylchiotobiose
-
 ?
3.2.1.96 N4-(beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-N2-dansyl-L-Asn + H2O
-
 Streptomyces plicatus beta-D-Man-(1->4)-beta-D-GlcNAc + N4-(beta-D-GlcNAc)-N2-dansyl-L-Asn
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.96 monomer 1 * 52000-54000, SDS-PAGE Streptomyces plicatus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.96 endo-beta-N-acetylglucosaminidase L
-
 Streptomyces plicatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.96 4 4.5 hydrolysis of Man(GlcNAc)2Asn-dansyl Streptomyces plicatus