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Literature summary extracted from

  • Ito, S.; Muramatsu, T.; Kobata, A.
    endo-beta-N-Acetylglucosaminidases acting on carbohydrate moieties of glycoproteins: purification and properties of the two enzymes with different specificities from Clostridium perfringens (1975), Arch. Biochem. Biophys., 171, 78-86.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.96 EDTA slight inhibition Clostridium perfringens
3.2.1.96 p-chloromercuriphenyl sulfonate
-
 Clostridium perfringens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.96 0.032
-
 Manalpha1->6(Manalpha1->3)Manbeta1->4GlcNAcbeta1->4(Fucalpha1->6)GlcNAc->N-acetylAsn endo-beta-N-acetylglucosaminidase CI Clostridium perfringens
3.2.1.96 0.05
-
 Manalpha1->6(Manalpha1->3)Manalpha1->6(Manalpha1->2Manalpha1->3)Manbeta1->4GlcNAcbeta1->4GlcNAcAsn endo-beta-N-acetylglucosaminidase CII Clostridium perfringens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.96 Clostridium perfringens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.96 endo-beta-N-acetylglucosaminidase CI and CII Clostridium perfringens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.96 culture medium
-
 Clostridium perfringens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.96 0.299
-
 endo-beta-N-acetylglucosaminidase CI Clostridium perfringens
3.2.1.96 0.304
-
 endo-beta-N-acetylglucosaminidase CII Clostridium perfringens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.96 (Man)6(GlcNAc)2Asn + H2O
-
 Clostridium perfringens (Man)6GlcNAc + GlcNAc-Asn
-
 ?
3.2.1.96 glycopeptide + H2O specificity Clostridium perfringens ?
-
 ?
3.2.1.96 Manalpha1->6(Manalpha1->3)Manalpha1->6(Manalpha1->2Manalpha1->3)Manbeta1->4GlcNAcbeta1->4GlcNAcAsn + H2O endo-beta-N-acetylglucosaminidase CII Clostridium perfringens Manalpha1->6(Manalpha1->3)Manalpha1->6(Manalpha1->2Manalpha1->3)Manbeta1->4GlcNAcbeta1 + GlcNAcAsn
-
 ?
3.2.1.96 Manalpha1->6(Manalpha1->3)Manbeta1->4GlcNAcbeta1->4(Fucalpha1->6)GlcNAc->N-acetylAsn + H2O endo-beta-N-acetylglucosaminidase CI Clostridium perfringens Manalpha1->6(Manalpha1->3)Manbeta1->4GlcNAc + Fucalpha1-6GlcNAc-N-acetylAsn
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.96 6.5
-
 endo-beta-N-acetylglucosaminidase CI Clostridium perfringens
3.2.1.96 7
-
 endo-beta-N-acetylglucosaminidase CI Clostridium perfringens