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Literature summary extracted from
- Enzymatic properties of endo-beta-N-acetylglucosaminidase from developing tomato fruits and soybean seeds: substrate specificity of plant origin endoglycosidase (1998), Biochim. Biophys. Acta, 1381, 27-36.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.96 | Cu2+ | 6 mM, 80% inhibition | Glycine max |  |
| 3.2.1.96 | Zn2+ | 6 mM, complete inhibition | Glycine max | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.96 | 62000 | - |
gel filtration | Glycine max |
| 3.2.1.96 | 62000 | - |
gel filtration | Solanum lycopersicum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.96 | Glycine max | - |
- |
- |
| 3.2.1.96 | Solanum lycopersicum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.96 | - |
Glycine max |
| 3.2.1.96 | - |
Solanum lycopersicum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.96 | fruit | - |
Solanum lycopersicum | - |
| 3.2.1.96 | seed | - |
Glycine max | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.96 | additional information | - |
- |
Glycine max |
| 3.2.1.96 | additional information | - |
- |
Solanum lycopersicum |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.96 | 0°C, stable for at least 1 month | Glycine max |
| 3.2.1.96 | 0°C, stable for at least 1 month | Solanum lycopersicum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.96 | (Man)3(Xyl)1(GlcNAc)2-pyridylamino + H2O | - |
Glycine max | ? | - |
? | |
| 3.2.1.96 | (Man)3(Xyl)1(GlcNAc)2-pyridylamino + H2O | - |
Solanum lycopersicum | ? | - |
? | |
| 3.2.1.96 | (Man)5(GlcNAc)2-pyridylamino + H2O | - |
Glycine max | (Man)5(GlcNAc) + 2-(acetylamino)-2-deoxy-N-pyridin-2-yl-beta-D-glucopyranosylamine | - |
? | |
| 3.2.1.96 | (Man)5(GlcNAc)2-pyridylamino + H2O | - |
Solanum lycopersicum | (Man)5(GlcNAc) + 2-(acetylamino)-2-deoxy-N-pyridin-2-yl-beta-D-glucopyranosylamine | - |
? | |
| 3.2.1.96 | (Man)6(GlcNAc)2-pyridylamino + H2O | - |
Glycine max | ? | - |
? | |
| 3.2.1.96 | (Man)6(GlcNAc)2-pyridylamino + H2O | - |
Solanum lycopersicum | ? | - |
? | |
| 3.2.1.96 | (Man)7(GlcNAc)2-pyridylamino + H2O | - |
Glycine max | ? | - |
? | |
| 3.2.1.96 | (Man)7(GlcNAc)2-pyridylamino + H2O | - |
Solanum lycopersicum | ? | - |
? | |
| 3.2.1.96 | (Man)8(GlcNAc)2-pyridylamino + H2O | - |
Glycine max | ? | - |
? | |
| 3.2.1.96 | (Man)8(GlcNAc)2-pyridylamino + H2O | - |
Solanum lycopersicum | ? | - |
? | |
| 3.2.1.96 | (Man)9(GlcNAc)2-pyridylamino + H2O | - |
Glycine max | ? | - |
? | |
| 3.2.1.96 | (Man)9(GlcNAc)2-pyridylamino + H2O | - |
Solanum lycopersicum | ? | - |
? | |
| 3.2.1.96 | beta-D-GlcNAc-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-3)-[alpha-D-Man-(1-6)]-alpha-D-Man-(1,6)]-beta-D-Man-(1-4)-beta-D-GlcNAc-(1-4)-beta-D-GlcNAc-pyridylamino | - |
Glycine max | ? | - |
? | |
| 3.2.1.96 | beta-D-GlcNAc-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-3)-[alpha-D-Man-(1-6)]-alpha-D-Man-(1,6)]-beta-D-Man-(1-4)-beta-D-GlcNAc-(1-4)-beta-D-GlcNAc-pyridylamino | - |
Solanum lycopersicum | ? | - |
? | |
| 3.2.1.96 | additional information | specificity | Glycine max | ? | - |
? | |
| 3.2.1.96 | additional information | specificity | Solanum lycopersicum | ? | - |
? | |
| 3.2.1.96 | additional information | description of substrate specificity, enzyme barely hydrolyzes xylose-containing N-glycans (Man)3(Xyl)1(GlcNAc)2-pyridylamino and (Man)3(Fuc)1(Xyl)1(GlcNAc)2-pyridylamino | Glycine max | ? | - |
? | |
| 3.2.1.96 | additional information | description of substrate specificity, enzyme barely hydrolyzes xylose-containing N-glycans (Man)3(Xyl)1(GlcNAc)2-pyridylamino and (Man)3(Fuc)1(Xyl)1(GlcNAc)2-pyridylamino | Solanum lycopersicum | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.96 | endo-GM | - |
Glycine max |
| 3.2.1.96 | endo-LE | - |
Solanum lycopersicum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.96 | 40 | - |
hydrolysis of (Man)6(GlcNAc)2-pyridylamino | Glycine max |
| 3.2.1.96 | 40 | - |
hydrolysis of (Man)6(GlcNAc)2-pyridylamino | Solanum lycopersicum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.96 | 6 | - |
hydrolysis of (Man)6(GlcNAc)2-pyridylamino | Solanum lycopersicum |
| 3.2.1.96 | 6.5 | - |
- |
Solanum lycopersicum |
| 3.2.1.96 | 6.5 | - |
hydrolysis of (Man)6(GlcNAc)2-pyridylamino | Glycine max |
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