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Literature summary extracted from

  • Schofield, L.R.; Daniel, R.M.
    Purification and properties of a beta-1,4-xylanase from a cellulolytic extreme thermophile expressed in Escherichia coli (1993), Int. J. Biochem., 25, 609-617.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 expression in Escherichia coli Caldicellulosiruptor saccharolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 iodoacetate 5 mM, 21% loss of activity Caldicellulosiruptor saccharolyticus
3.2.1.8 Ni2+ 5 mM, complete inhibition Caldicellulosiruptor saccharolyticus
3.2.1.8 RbCl 5 mM, 55% inhibition Caldicellulosiruptor saccharolyticus
3.2.1.8 SDS 1% w/w, 86% loss of activity Caldicellulosiruptor saccharolyticus
3.2.1.8 xylose no inhibition Caldicellulosiruptor saccharolyticus
3.2.1.8 Zn2+ 5 mM, complete inhibition Caldicellulosiruptor saccharolyticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 K+ 200 mM, slight activation Caldicellulosiruptor saccharolyticus
3.2.1.8 Li+ 200 mM, slight activation Caldicellulosiruptor saccharolyticus
3.2.1.8 Mg2+ 5 mM MgCl2, slight activation Caldicellulosiruptor saccharolyticus
3.2.1.8 Na+ 200 mM NaCl, slight activation Caldicellulosiruptor saccharolyticus
3.2.1.8 SrCl2 5 mM, slight activation Caldicellulosiruptor saccharolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 32000
-
 gel filtration Caldicellulosiruptor saccharolyticus
3.2.1.8 42000
-
 1 * 42000, SDS-PAGE Caldicellulosiruptor saccharolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Caldicellulosiruptor saccharolyticus
-
-
-
3.2.1.8 Caldicellulosiruptor saccharolyticus Tp8T6.3.3.1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 recombinant enzyme expressed in Escherichia coli Caldicellulosiruptor saccharolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 167
-
-
 Caldicellulosiruptor saccharolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 1,4-beta-D-xylan + H2O low activity on larchwood xylan Caldicellulosiruptor saccharolyticus additional information release of xylobiose and some xylotriose from oat spelt xylan ?
3.2.1.8 1,4-beta-D-xylan + H2O larchwood xylan Caldicellulosiruptor saccharolyticus additional information release of xylobiose and some xylotriose from oat spelt xylan ?
3.2.1.8 1,4-beta-D-xylan + H2O oat spelt xylan Caldicellulosiruptor saccharolyticus additional information release of xylobiose and some xylotriose from oat spelt xylan ?
3.2.1.8 1,4-beta-D-xylan + H2O low activity on larchwood xylan Caldicellulosiruptor saccharolyticus Tp8T6.3.3.1 additional information release of xylobiose and some xylotriose from oat spelt xylan ?
3.2.1.8 1,4-beta-D-xylan + H2O larchwood xylan Caldicellulosiruptor saccharolyticus Tp8T6.3.3.1 additional information release of xylobiose and some xylotriose from oat spelt xylan ?
3.2.1.8 1,4-beta-D-xylan + H2O oat spelt xylan Caldicellulosiruptor saccharolyticus Tp8T6.3.3.1 additional information release of xylobiose and some xylotriose from oat spelt xylan ?
3.2.1.8 4-methylumbelliferyl beta-D-cellobioside + H2O
-
 Caldicellulosiruptor saccharolyticus ?
-
 ?
3.2.1.8 acetylhemicellulose + H2O high activity on sugar cane bagasse hemicellulose A and B Caldicellulosiruptor saccharolyticus hemicellulose + acetate
-
 ?
3.2.1.8 acetylhemicellulose + H2O high activity on sugar cane bagasse hemicellulose A and B Caldicellulosiruptor saccharolyticus Tp8T6.3.3.1 hemicellulose + acetate
-
 ?
3.2.1.8 carboxymethylcellulose + H2O
-
 Caldicellulosiruptor saccharolyticus ?
-
 ?
3.2.1.8 p-nitrophenyl beta-D-cellobioside + H2O
-
 Caldicellulosiruptor saccharolyticus ?
-
 ?
3.2.1.8 p-nitrophenyl beta-D-xylopyranoside transferase activity on Caldicellulosiruptor saccharolyticus ?
-
 ?
3.2.1.8 xylobiose + H2O no hydrolysis Caldicellulosiruptor saccharolyticus ?
-
 ?
3.2.1.8 xylobiose + H2O no hydrolysis Caldicellulosiruptor saccharolyticus Tp8T6.3.3.1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 monomer 1 * 42000, SDS-PAGE Caldicellulosiruptor saccharolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 70
-
-
 Caldicellulosiruptor saccharolyticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 40 80 40°C: about 30% of maximal activity, 80°C: about 20% of maximal activity Caldicellulosiruptor saccharolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 70
-
 pH 6.0, half-life: 4 min in absence of added protein, 30 min, in presence of 0.5 mg/ml bovine serum albumin Caldicellulosiruptor saccharolyticus
3.2.1.8 80
-
 pH 6.0, half-life is 20 min in presence of 0.5 mg/ml bovine serum albumin Caldicellulosiruptor saccharolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6.5
-
-
 Caldicellulosiruptor saccharolyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 5 7.7 more than 50% of maximal activity at pH 5.0 and at pH 7.0 Caldicellulosiruptor saccharolyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 4.5 11.5 stable at room temperature in presence of 0.5 mg/ml bovine serum albumin Caldicellulosiruptor saccharolyticus