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Literature summary extracted from

  • Jones, G.H.; Ballou, C.E.
    Studies on the structure of yeast mannan. I. Purification and some properties of an alpha-mannosidase from an Arthrobacter species (1969), J. Biol. Chem., 244, 1043-1051.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.77 Cu2+
-
Arthrobacter sp.
3.2.1.77 EDTA
-
Arthrobacter sp.
3.2.1.77 Mg2+
-
Arthrobacter sp.
3.2.1.77 Zn2+
-
Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.77 0.01
-
Mannan based on molecular weight of 22000 from intact S. cerevisiae mannan Arthrobacter sp.
3.2.1.77 0.5 2 oligosaccharides from mannan molecular weight of mannan multiplied by factor 50 describes molar concentration of side chains in the mannan molecule Arthrobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.77 extracellular
-
Arthrobacter sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.77 Ca2+ restores activity after EDTA treatment Arthrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.77 mannan + H2O Arthrobacter sp.
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.77 Arthrobacter sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.77 partially Arthrobacter sp.

Storage Stability

EC Number Storage Stability Organism
3.2.1.77 -10°C, indefinitely, Arthrobacter sp.
3.2.1.77 20°C, one drop of toluene, 1 day, 15% decline in activity Arthrobacter sp.
3.2.1.77 4°C, 2 days, Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.77 mannan + H2O
-
Arthrobacter sp. mannose + 1,6-alpha-D-mannan-backbone further slow degradation ?
3.2.1.77 mannan + H2O
-
Arthrobacter sp. ?
-
?
3.2.1.77 oligosaccharides from mannan + H2O
-
Arthrobacter sp. mannose + ?
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.77 70
-
not stable above Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.77 6.5 7 mannan Arthrobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.77 5 9 mannan Arthrobacter sp.