EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.33 | additional information | no activating by usual sugar phosphates or nucleotide effectors of glycolysis such as AMP, ADP, ATP, uridine diphosphoglucose, glucose-1-phosphate, glucose-6-phosphate and phosphate | Squalus acanthias |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.33 | 5,5'-dithiobis(2-nitrobenzoic acid) | 50% inhibition, reversed by glycogen | Squalus acanthias | |
3.2.1.33 | additional information | no inhibition by usual sugar phosphates or nucleotide effectors of glycolysis such as AMP, ADP, ATP, uridine diphosphoglucose, glucose-1-phosphate, glucose-6-phosphate and phosphate | Squalus acanthias | |
3.2.1.33 | Tris | 15 mM: 75% inhibition | Squalus acanthias |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.33 | 160000 | - |
glycogen debranching enzyme, PAGE and SDS-PAGE | Squalus acanthias |
3.2.1.33 | 162000 | - |
glycogen debranching enzyme, carboxymethylation prior to sedimentation equilibrium | Squalus acanthias |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.33 | additional information | Squalus acanthias | glycogen debranching enzyme might play an important role in the structure of glycogen particles, or the control of its metabolism | ? | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.2.1.33 | Ethanol | stable in ethanol precipitation with final concentration of 23% ethanol | Squalus acanthias |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.33 | Squalus acanthias | - |
pacific dogfish | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.33 | no modification | no covalently bound phosphate or carbohydrate, method would not have detected less than 5% carbohydrate | Squalus acanthias |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.33 | glycogen debranching enzyme | Squalus acanthias |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.2.1.33 | 6-O-alpha-D-glucosyl cyclomaltoheptaose + H2O = D-glucose + cyclomaltoheptaose | regulation of enzyme activity | Squalus acanthias |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.33 | muscle | - |
Squalus acanthias | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.33 | 3 | - |
maleate buffer, pH 6.0, 19°C | Squalus acanthias |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.33 | frozen, solution in Tris-glycerol buffer, 3 months, without loss of activity | Squalus acanthias |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.33 | alpha-(1-6)-glucosyl cyclohexaamylose + H2O | - |
Squalus acanthias | cyclohexaamylose + D-glucose | - |
r | |
3.2.1.33 | glycogen + H2O | liver glycogen and shellfish glycogen: one-tenth of activity compared with glycogen phosphorylase limit dextrin as substrate | Squalus acanthias | glycogen + D-glucose | - |
r | |
3.2.1.33 | glycogen phosphorylase-limit dextrin + H2O | - |
Squalus acanthias | limit dextrin + D-glucose | - |
r | |
3.2.1.33 | additional information | glycogen debranching enzyme might play an important role in the structure of glycogen particles, or the control of its metabolism | Squalus acanthias | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.33 | monomer | 1 * 160000, glycogen debranching enzyme, carboxymethylation prior to SDS-PAGE | Squalus acanthias |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.33 | glycogen debranching system | EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system | Squalus acanthias |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.33 | 19 | - |
- |
Squalus acanthias |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.33 | 20 | - |
unstable above | Squalus acanthias |
3.2.1.33 | 30 | - |
rapid inactivation | Squalus acanthias |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.33 | 5.5 | 6.3 | - |
Squalus acanthias |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.33 | 4.6 | 9 | inactive below pH 4.6 and above pH 9 | Squalus acanthias |