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Literature summary extracted from

  • Brown, D.H.; Illingworth Brown, B.
    Enzymes of glycogen debranching: amylo-1,6-glucosidase (I) and oligo-1,4 -> 1,4-glucantransferase (II) (1966), Methods Enzymol., 8, 515-524.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.33 additional information no activating substance Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.33 glycylglycine slight inhibition Oryctolagus cuniculus
3.2.1.33 p-hydroxymercuribenzoate 0.3 mM: completely Oryctolagus cuniculus
3.2.1.33 Tris 0.02 M Tris: 50-60% inhibition Oryctolagus cuniculus
3.2.1.33 Urea glucosidase-transferase: 31% inhibition at 0.21 M, 72% at 1.6 M, 98% at 3.2 M Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.33 additional information
-
 additional information
-
 Oryctolagus cuniculus
3.2.1.33 0.74
-
 glycogen phosphorylase limit dextrin
-
 Oryctolagus cuniculus
3.2.1.33 1.8
-
 63-alpha-glucosyl maltotetraose branched pentasaccharide B5, pH 6.6 Oryctolagus cuniculus
3.2.1.33 5.6
-
 63-alpha-maltotriosyl maltotetraose branched heptasaccharide B7, muscle enzyme Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.33 267000 279000 glycogen debranching enzyme, equilibrium ultracentrifugation Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.33 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.33
-
 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.33 muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.33 additional information
-
-
 Oryctolagus cuniculus
3.2.1.33 0.82
-
 substrate: 63-alpha-glucosyl maltotetraose Oryctolagus cuniculus
3.2.1.33 3.9
-
 substrate: limit dextrin Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.33 63-alpha-glucosyl maltotetraose + H2O branched pentasaccharide "fast B5" Oryctolagus cuniculus maltotetraose + D-glucose
-
 r
3.2.1.33 63-alpha-maltotriosyl maltotetraose + H2O branched heptasaccharide B7 Oryctolagus cuniculus ?
-
 ?
3.2.1.33 beta-amylase limit dextrin + H2O
-
 Oryctolagus cuniculus limit dextrin + D-glucose
-
 r
3.2.1.33 glycogen phosphorylase limit dextrin + H2O
-
 Oryctolagus cuniculus ?
-
 ?
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O
-
 Oryctolagus cuniculus limit dextrin + D-glucose partially debranched glycogen r
3.2.1.33 additional information
-
 Oryctolagus cuniculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.33 glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.33 37
-
-
 Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.33 6.1 6.4 sodium citrate/mercaptoethanol buffer Oryctolagus cuniculus