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Literature summary extracted from

  • Gordon, R.B.; Brown, D.H.; Illingworth Brown, B.
    Preparation and properties of the glycogen-debranching enzyme from rabbit liver (1972), Biochim. Biophys. Acta, 289, 97-107.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.33 stable in 2 M urea, irreversible denaturation with higher concentrations, 3 M, of urea Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.33 guanidine at 0.05 M: 20% inhibition, at 0.15 M: 50% inhibition Oryctolagus cuniculus
3.2.1.33 imidazole buffer, 50% inhibition Oryctolagus cuniculus
3.2.1.33 Tris buffer, strong inhibition Oryctolagus cuniculus
3.2.1.33 Urea muscle enzyme: reversible 95% inhibition by 2 M urea, with higher concentration, 3 M urea: time-dependent irreversible denaturation, liver enzyme: reversible 57% inhibition by 2 M urea; reversible inhibitor below 2 M, 90% inhibition by 2.6 M urea Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.33 179300
-
 glycogen debranching enzyme, liver enzyme, sucrose density gradient centrifugation Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.33 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.33 glycogen debranching enzyme Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.33 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.33 4.5
-
 liver enzyme Oryctolagus cuniculus
3.2.1.33 5.86
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.33 63-alpha-glucosyl maltotetraose + H2O branched pentasaccharide "fast B5" Oryctolagus cuniculus maltotetraose + D-glucose
-
 r
3.2.1.33 63-alpha-maltotriosyl maltotetraose + H2O branched heptasaccharide B7 Oryctolagus cuniculus ?
-
 ?
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O
-
 Oryctolagus cuniculus limit dextrin + D-glucose
-
 r

Synonyms

EC Number Synonyms Comment Organism
3.2.1.33 glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.33 additional information
-
 pH-optimum depends on type of buffer Oryctolagus cuniculus
3.2.1.33 5.4
-
 imidazole buffer, about 50% inhibition Oryctolagus cuniculus
3.2.1.33 6
-
 substrate: glycogen phosphorylase limit dextrin, citrate /phosphate buffer Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.33 5 8
-
 Oryctolagus cuniculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.33 0.0015
-
 guanidine
-
 Oryctolagus cuniculus