BRENDA - Enzyme Database

Quarternary structure, Mg2+ interactions, and some kinetic properties of the beta-galactosidase from Thermoanaerobacterium thermosulfurigenes EM1

Huber, R.E.; Roth, N.J.; Bahl, H.; J. Protein Chem. 15, 621-629 (1996)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.23
2-deoxy-D-galactose
-
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
D-arabinose
-
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
D-galactono-1,4-lactone
-
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
D-galactose
-
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
D-glucose
-
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
D-Lyxose
-
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
glycerol
-
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
isopropyl thiogalactoside
-
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
lactose
-
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
ribose
-
Thermoanaerobacterium thermosulfurigenes
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.23
0.37
-
p-nitrophenyl-beta-D-galactoside
at 50°C, in presence of 10 mM Mg2+
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
0.39
-
p-nitrophenyl-beta-D-galactoside
at 25°C, in presence of 10 mM Mg2+
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
0.58
-
o-nitrophenyl-beta-D-galactoside
at 50°C, in presence of 10 mM Mg2+
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
0.64
-
o-nitrophenyl-beta-D-galactoside
at 25°C, in presence of 10 mM Mg2+
Thermoanaerobacterium thermosulfurigenes
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.23
K+
no effect
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
Mg2+
contains 0.19 Mg2+ per monomer
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
Na+
no effect
Thermoanaerobacterium thermosulfurigenes
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.23
175000
178000
gel filtration, non-denaturing PAGE
Thermoanaerobacterium thermosulfurigenes
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.23
Thermoanaerobacterium thermosulfurigenes
-
-
-
3.2.1.23
Thermoanaerobacterium thermosulfurigenes EM1
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.23
o-nitrophenyl-beta-D-galactoside + H2O
-
171346
Thermoanaerobacterium thermosulfurigenes
o-nitrophenol + beta-D-galactose
-
-
-
?
3.2.1.23
o-nitrophenyl-beta-D-galactoside + H2O
-
171346
Thermoanaerobacterium thermosulfurigenes EM1
o-nitrophenol + beta-D-galactose
-
-
-
?
3.2.1.23
p-nitrophenyl-beta-D-galactoside + H2O
-
171346
Thermoanaerobacterium thermosulfurigenes
p-nitrophenol + beta-D-galactose
-
-
-
?
3.2.1.23
p-nitrophenyl-beta-D-galactoside + H2O
-
171346
Thermoanaerobacterium thermosulfurigenes EM1
p-nitrophenol + beta-D-galactose
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.23
42.4
-
p-nitrophenyl-beta-D-galactoside
at 25°C, in presence of 10 mM Mg2+
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
54.5
-
o-nitrophenyl-beta-D-galactoside
at 25°C, in presence of 10 mM Mg2+
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
264
-
o-nitrophenyl-beta-D-galactoside
at 50°C, in presence of 10 mM Mg2+
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
312
-
p-nitrophenyl-beta-D-galactoside
at 50°C, in presence of 10 mM Mg2+
Thermoanaerobacterium thermosulfurigenes
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.23
6.5
-
-
Thermoanaerobacterium thermosulfurigenes
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.23
2-deoxy-D-galactose
-
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
D-arabinose
-
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
D-galactono-1,4-lactone
-
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
D-galactose
-
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
D-glucose
-
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
D-Lyxose
-
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
glycerol
-
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
isopropyl thiogalactoside
-
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
lactose
-
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
ribose
-
Thermoanaerobacterium thermosulfurigenes
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.23
0.37
-
p-nitrophenyl-beta-D-galactoside
at 50°C, in presence of 10 mM Mg2+
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
0.39
-
p-nitrophenyl-beta-D-galactoside
at 25°C, in presence of 10 mM Mg2+
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
0.58
-
o-nitrophenyl-beta-D-galactoside
at 50°C, in presence of 10 mM Mg2+
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
0.64
-
o-nitrophenyl-beta-D-galactoside
at 25°C, in presence of 10 mM Mg2+
Thermoanaerobacterium thermosulfurigenes
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.23
K+
no effect
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
Mg2+
contains 0.19 Mg2+ per monomer
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
Na+
no effect
Thermoanaerobacterium thermosulfurigenes
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.23
175000
178000
gel filtration, non-denaturing PAGE
Thermoanaerobacterium thermosulfurigenes
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.23
o-nitrophenyl-beta-D-galactoside + H2O
-
171346
Thermoanaerobacterium thermosulfurigenes
o-nitrophenol + beta-D-galactose
-
-
-
?
3.2.1.23
o-nitrophenyl-beta-D-galactoside + H2O
-
171346
Thermoanaerobacterium thermosulfurigenes EM1
o-nitrophenol + beta-D-galactose
-
-
-
?
3.2.1.23
p-nitrophenyl-beta-D-galactoside + H2O
-
171346
Thermoanaerobacterium thermosulfurigenes
p-nitrophenol + beta-D-galactose
-
-
-
?
3.2.1.23
p-nitrophenyl-beta-D-galactoside + H2O
-
171346
Thermoanaerobacterium thermosulfurigenes EM1
p-nitrophenol + beta-D-galactose
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.23
42.4
-
p-nitrophenyl-beta-D-galactoside
at 25°C, in presence of 10 mM Mg2+
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
54.5
-
o-nitrophenyl-beta-D-galactoside
at 25°C, in presence of 10 mM Mg2+
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
264
-
o-nitrophenyl-beta-D-galactoside
at 50°C, in presence of 10 mM Mg2+
Thermoanaerobacterium thermosulfurigenes
3.2.1.23
312
-
p-nitrophenyl-beta-D-galactoside
at 50°C, in presence of 10 mM Mg2+
Thermoanaerobacterium thermosulfurigenes
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.23
6.5
-
-
Thermoanaerobacterium thermosulfurigenes