Literature summary extracted from

Holmes, E.W.; O'Brien, J.S.
Purification and properties of acid beta-galactosidase from feline liver (1979), Biochemistry, 18, 952-958.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.2.1.23	Hg2+	0.2 mM, complete inhibition	Felis catus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.2.1.23	0.14	-	4-methylumbelliferyl-beta-D-galactoside	-	Felis catus
3.2.1.23	0.17	-	ganglioside GM1	-	Felis catus
3.2.1.23	0.62	-	p-nitrophenyl-beta-D-galactoside	-	Felis catus
3.2.1.23	1.5	-	asialofetuin	-	Felis catus
3.2.1.23	2.16	-	4-methylumbelliferyl-alpha-L-arabinoside	-	Felis catus
3.2.1.23	56.9	-	o-nitrophenyl beta-D-fucoside	-	Felis catus

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
3.2.1.23	Na+	hydrolysis of 4-methylumbelliferyl-beta-D-galactoside is mildly stimulated by NaCl	Felis catus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.2.1.23	62000	-	2 * 62000, SDS-PAGE of reduced enzyme	Felis catus
3.2.1.23	115000	-	sucrose density gradient centrifugation	Felis catus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.23	Felis catus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.2.1.23	-	Felis catus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.2.1.23	liver	-	Felis catus	-

Storage Stability

EC Number	Storage Stability	Organism
3.2.1.23	4°C, protein concentration 1.2 mg/ml, stable for 8 months	Felis catus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.1.23	4-methylumbelliferyl alpha-L-arabinoside + H2O	-	Felis catus	4-methylumbelliferone + alpha-L-arabinose	-	?
3.2.1.23	4-methylumbelliferyl-beta-D-galactoside + H2O	-	Felis catus	4-methylumbelliferone + beta-D-galactose	-	?
3.2.1.23	asialofetuin + H2O	-	Felis catus	beta-D-galactose + ?	-	?
3.2.1.23	glycolipid GM1 ganglioside + H2O	-	Felis catus	beta-D-galactose + ?	-	?
3.2.1.23	additional information	transfer of galactose from p-nitrophenyl-beta-D-galactoside and ganglioside GM1 to various carbohydrate acceptors	Felis catus	?	-	?
3.2.1.23	o-nitrophenyl beta-D-fucoside + H2O	-	Felis catus	o-nitrophenol + beta-D-fucose	-	?
3.2.1.23	p-nitrophenyl-beta-D-galactoside + H2O	-	Felis catus	p-nitrophenol + beta-D-galactose	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.2.1.23	dimer	2 * 62000, SDS-PAGE of reduced enzyme	Felis catus

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Release 2023.1 (January 2023)