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Literature summary extracted from

  • Horowitz, B.; Meister, A.
    Glutamine-dependent asparagine synthetase from leukemia cells. Chloride dependence mechanism of action and inhibition (1972), J. Biol. Chem., 247, 6708-6719.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.4 2,3-dicarboxypyridine
-
 Mus musculus
6.3.5.4 2,4-Dicarboxypyridine
-
 Mus musculus
6.3.5.4 2,5-Dicarboxypyridine
-
 Mus musculus
6.3.5.4 2,6-dicarboxypyridine
-
 Mus musculus
6.3.5.4 2-carboxypyridine
-
 Mus musculus
6.3.5.4 3,4-Dicarboxypyridine
-
 Mus musculus
6.3.5.4 3,5-Dicarboxypyridine
-
 Mus musculus
6.3.5.4 4-Carboxypyridine
-
 Mus musculus
6.3.5.4 Albizzine
-
 Mus musculus
6.3.5.4 alpha,beta-methylene ATP
-
 Mus musculus
6.3.5.4 aminomalonic acid
-
 Mus musculus
6.3.5.4 ammonium maleamate weak Mus musculus
6.3.5.4 AMP
-
 Mus musculus
6.3.5.4 Arg
-
 Mus musculus
6.3.5.4 Asn
-
 Mus musculus
6.3.5.4 beta,gamma-methylene ATP
-
 Mus musculus
6.3.5.4 D-Aspartic acid weak Mus musculus
6.3.5.4 diphosphate
-
 Mus musculus
6.3.5.4 DL-alpha-Aminotricarballylic acid weak Mus musculus
6.3.5.4 Glu
-
 Mus musculus
6.3.5.4 Gly-L-Asn weak Mus musculus
6.3.5.4 L-2-Amino-4-oxo-5-chloropentanoic acid
-
 Mus musculus
6.3.5.4 L-2-Amino-4-oxo-5-hydroxypentanoic acid
-
 Mus musculus
6.3.5.4 L-beta-Aspartate ethyl ester
-
 Mus musculus
6.3.5.4 L-Malic acid weak Mus musculus
6.3.5.4 N-Benzyloxycarbonyl-L-Asn weak Mus musculus
6.3.5.4 N-Benzyloxycarbonyl-L-aspartate weak Mus musculus
6.3.5.4 N-Methyl-DL-aspartic acid
-
 Mus musculus
6.3.5.4 Phthalic acid weak Mus musculus
6.3.5.4 S-Carbamoylcysteine
-
 Mus musculus
6.3.5.4 S-methyl-L-cysteine
-
 Mus musculus
6.3.5.4 S-methyl-L-cysteine-(RS)-sulfoximine weak Mus musculus
6.3.5.4 Trp
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.4 0.2
-
 ATP
-
 Mus musculus
6.3.5.4 0.9
-
 L-Asp
-
 Mus musculus
6.3.5.4 1
-
 L-Gln
-
 Mus musculus
6.3.5.4 9
-
 NH4+
-
 Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.4 Br- can partially replace Cl- in activation Mus musculus
6.3.5.4 Cl- required for glutaminase and glutamine-dependent synthetase activity, not for the NH4+-dependent activity Mus musculus
6.3.5.4 I- can partially replace Cl- in activation Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.4 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.4
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.5.4 leukemia cell line Asn-resistant leukemia cells Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.4 ATP + L-Asp + L-Gln
-
 Mus musculus AMP + diphosphate + Asn + Glu
-
 ?
6.3.5.4 ATP + L-Asp + NH3
-
 Mus musculus AMP + diphosphate + Asn
-
 ?
6.3.5.4 additional information enzyme has inherent glutaminase activity Mus musculus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.4 7.6 8.2
-
 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.5.4 6.7 8.8 minor variation of activity in this pH range Mus musculus