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Literature summary extracted from

  • Pressey, R.
    Tomato alpha-Galactosidases: conversion of human type B erythrocytes to type O (1984), Phytochemistry, 23, 55-58.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.22
-
 p-nitrophenyl alpha-D-galactoside alpha-galactosidase II Solanum lycopersicum
3.2.1.22 0.54
-
 p-nitrophenyl alpha-D-galactoside alpha-galactosidase I Solanum lycopersicum
3.2.1.22 1.8
-
 raffinose alpha-galactosidase I Solanum lycopersicum
3.2.1.22 2.4
-
 stachyose alpha-galactosidase I Solanum lycopersicum
3.2.1.22 2.7
-
 raffinose alpha-galactosidase II Solanum lycopersicum
3.2.1.22 3.8
-
 stachyose alpha-galactosidase II Solanum lycopersicum
3.2.1.22 5.3
-
 methyl alpha-D-galactoside alpha-galactosidase I Solanum lycopersicum
3.2.1.22 8.4
-
 methyl alpha-D-galactoside alpha-galactosidase II Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 19000
-
 alpha-galactosidase II, gel filtration Solanum lycopersicum
3.2.1.22 44000
-
 alpha-galactosidase I, gel filtration Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Solanum lycopersicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22
-
 Solanum lycopersicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 2.3
-
 alpha-galactosidase I Solanum lycopersicum
3.2.1.22 82
-
 alpha-galactosidase II Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 galactomannan + H2O
-
 Solanum lycopersicum ?
-
 ?
3.2.1.22 methyl alpha-D-galactoside + H2O
-
 Solanum lycopersicum methanol + D-galactose
-
 ?
3.2.1.22 p-nitrophenyl-alpha-D-galactoside + H2O
-
 Solanum lycopersicum p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 raffinose + H2O
-
 Solanum lycopersicum sucrose + D-galactose
-
 ?
3.2.1.22 stachyose + H2O
-
 Solanum lycopersicum ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 55
-
 5 min, pH 4.5, 50% inactivation, alpha-galactosidase II Solanum lycopersicum
3.2.1.22 70
-
 5 min, pH 5.2, 50% inactivation, alpha-galactosidase I Solanum lycopersicum