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Literature summary extracted from

  • Dey, P.M.; Pridham, J.B.
    Biochemistry of alpha-galactosidases (1972), Adv. Enzymol. Relat. Areas Mol. Biol., 36, 91-130.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.22 Mn2+ and reducing agents stabilizes activity in cell free extract Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Ag+
-
 Medicago sativa
3.2.1.22 Ag+
-
 Prunus dulcis
3.2.1.22 Ag+
-
 Vicia faba
3.2.1.22 Cu2+
-
 Prunus dulcis
3.2.1.22 Hg2+
-
 Medicago sativa
3.2.1.22 Hg2+
-
 Prunus dulcis
3.2.1.22 Hg2+
-
 Umbelopsis vinacea
3.2.1.22 Hg2+
-
 Vicia faba
3.2.1.22 iodacetamide
-
 Klebsiella aerogenes
3.2.1.22 iodacetamide
-
 Streptococcus pneumoniae
3.2.1.22 iodacetamide
-
 Streptomyces olivaceus
3.2.1.22 N-ethyl maleimide
-
 Klebsiella aerogenes
3.2.1.22 N-ethyl maleimide
-
 Streptococcus pneumoniae
3.2.1.22 N-ethyl maleimide
-
 Streptomyces olivaceus
3.2.1.22 p-chloromercuribenzoate
-
 Klebsiella aerogenes
3.2.1.22 p-chloromercuribenzoate
-
 Streptococcus pneumoniae
3.2.1.22 p-chloromercuribenzoate
-
 Streptomyces olivaceus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.13
-
 galactinol alpha-galactosidase I Vicia faba
3.2.1.22 0.33
-
 o-nitrophenyl alpha-D-galactoside
-
 Prunus dulcis
3.2.1.22 0.38
-
 p-nitrophenyl alpha-D-galactoside alpha-galactosidase I Vicia faba
3.2.1.22 0.45
-
 p-nitrophenyl alpha-D-galactoside alpha-galactosidase II Vicia faba
3.2.1.22 0.53
-
 p-nitrophenyl alpha-D-galactoside
-
 Prunus dulcis
3.2.1.22 0.62
-
 6-bromo-2-naphthyl alpha-D-galactoside alpha-galactosidase II Vicia faba
3.2.1.22 0.69
-
 galactinol alpha-galactosidase II Vicia faba
3.2.1.22 0.69
-
 o-nitrophenyl alpha-D-galactoside alpha-galactosidase II Vicia faba
3.2.1.22 0.77
-
 melibiose alpha-galactosidase II Vicia faba
3.2.1.22 0.78
-
 o-cresyl alpha-D-galactoside alpha-galactosidase II Vicia faba
3.2.1.22 0.83
-
 m-chlorophenyl alpha-D-galactoside alpha-galactosidase I Vicia faba
3.2.1.22 0.87
-
 p-aminophenyl alpha-D-galactoside alpha-galactosidase II Vicia faba
3.2.1.22 0.95
-
 p-aminophenyl alpha-D-galactoside alpha-galactosidase I Vicia faba
3.2.1.22 0.96
-
 melibiose alpha-galactosidase I Vicia faba
3.2.1.22 1
-
 p-cresyl alpha-D-galactoside alpha-galactosidase II Vicia faba
3.2.1.22 1.11
-
 phenyl alpha-D-galactoside alpha-galactosidase I Vicia faba
3.2.1.22 1.14
-
 o-nitrophenyl alpha-D-galactoside alpha-galactosidase I Vicia faba
3.2.1.22 1.17
-
 m-chlorophenyl alpha-D-galactoside alpha-galactosidase II Vicia faba
3.2.1.22 1.25
-
 phenyl alpha-D-galactoside alpha-galactosidase II Vicia faba
3.2.1.22 1.33
-
 o-cresyl alpha-D-galactoside alpha-galactosidase I Vicia faba
3.2.1.22 1.38
-
 m-cresyl alpha-D-galactoside alpha-galactosidase I Vicia faba
3.2.1.22 1.54
-
 p-cresyl alpha-D-galactoside alpha-galactosidase I Vicia faba
3.2.1.22 1.57
-
 m-nitrophenyl alpha-D-galactoside
-
 Prunus dulcis
3.2.1.22 1.8
-
 6-bromo-2-naphthyl alpha-D-galactoside alpha-galactosidase I Vicia faba
3.2.1.22 2
-
 m-cresyl alpha-D-galactoside alpha-galactosidase II Vicia faba
3.2.1.22 2.24
-
 melibiose
-
 Prunus dulcis
3.2.1.22 2.5
-
 m-nitrophenyl alpha-D-galactoside alpha-galactosidase II Vicia faba
3.2.1.22 4
-
 raffinose alpha-galactosidase I Vicia faba
3.2.1.22 4.54
-
 o-cresyl alpha-D-galactoside
-
 Prunus dulcis
3.2.1.22 4.76
-
 p-cresyl alpha-D-galactoside
-
 Prunus dulcis
3.2.1.22 4.76
-
 p-nitrophenyl alpha-D-fucoside alpha-galactosidase I Vicia faba
3.2.1.22 5
-
 phenyl alpha-D-galactoside
-
 Prunus dulcis
3.2.1.22 5
-
 raffinose alpha-galactosidase II Vicia faba
3.2.1.22 5.26
-
 stachyose alpha-galactosidase II Vicia faba
3.2.1.22 5.88
-
 n-propyl alpha-D-galactoside alpha-galactosidase II Vicia faba
3.2.1.22 5.88
-
 p-nitrophenyl alpha-D-fucoside alpha-galactosidase II Vicia faba
3.2.1.22 6.13
-
 n-propyl alpha-D-galactoside alpha-galactosidase I Vicia faba
3.2.1.22 6.25
-
 ethyl alpha-D-galactoside
-
 Prunus dulcis
3.2.1.22 6.25
-
 n-propyl alpha-D-galactoside
-
 Prunus dulcis
3.2.1.22 7.13
-
 methyl alpha-D-galactoside alpha-galactosidase I Vicia faba
3.2.1.22 7.5
-
 stachyose alpha-galactosidase I Vicia faba
3.2.1.22 8
-
 ethyl alpha-D-galactoside alpha-galactosidase II Vicia faba
3.2.1.22 8.33
-
 m-chlorophenyl alpha-D-galactoside
-
 Prunus dulcis
3.2.1.22 8.33
-
 m-cresyl alpha-D-galactoside
-
 Prunus dulcis
3.2.1.22 8.93
-
 ethyl alpha-D-galactoside alpha-galactosidase I Vicia faba
3.2.1.22 10
-
 m-nitrophenyl alpha-D-galactoside alpha-galactosidase I Vicia faba
3.2.1.22 10.9
-
 methyl alpha-D-galactoside
-
 Prunus dulcis
3.2.1.22 12.5
-
 raffinose
-
 Prunus dulcis
3.2.1.22 12.5
-
 p-nitrophenyl beta-L-arabinoside alpha-galactosidase II Vicia faba
3.2.1.22 14.3
-
 p-nitrophenyl beta-L-arabinoside alpha-galactosidase I Vicia faba
3.2.1.22 14.3
-
 methyl alpha-D-galactoside alpha-galactosidase II Vicia faba
3.2.1.22 33.3
-
 p-nitrophenyl beta-L-arabinoside
-
 Prunus dulcis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.22 chloroplast
-
 Spinacia oleracea 9507
-
3.2.1.22 cytoplasm
-
 Rattus norvegicus 5737
-
3.2.1.22 mitochondrion
-
 Rattus norvegicus 5739
-
3.2.1.22 mitochondrion
-
 Spinacia oleracea 5739
-
3.2.1.22 particle-bound
-
 Sus scrofa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.22 K+ activates the enzyme Vicia faba

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 23000
-
 gel filtration Helianthus annuus
3.2.1.22 25000
-
 gel filtration Cyamopsis tetragonoloba
3.2.1.22 26000
-
 gel filtration Coffea sp.
3.2.1.22 30000
-
 germinated seeds, gel filtration Vicia sativa
3.2.1.22 30000 32400 gel filtration Prunus dulcis
3.2.1.22 32300
-
 alpha-galactosidase II, gel filtration Pisum sativum
3.2.1.22 38000
-
 gel filtration Medicago sativa
3.2.1.22 38000
-
 alpha-galactosidase II, gel filtration Vigna radiata var. radiata
3.2.1.22 38000
-
 alpha-galactosidase II, gel filtration Vicia faba
3.2.1.22 39800
-
 alpha-galactosidase II, gel filtration Phaseolus vulgaris
3.2.1.22 50000
-
 alpha-galactosidase II, gel filtration Acer pseudoplatanus
3.2.1.22 57000
-
 alpha-galactosidase II, gel filtration Vicia dumetorum
3.2.1.22 77000
-
 dormant seeds, alpha-galactosidase II, gel filtration Vicia sativa
3.2.1.22 87000
-
 gel filtration Cajanus indicus
3.2.1.22 121600
-
 alpha-galactosidase I, gel filtration Pisum sativum
3.2.1.22 125900
-
 alpha-galactosidase I, gel filtration Phaseolus vulgaris
3.2.1.22 159000
-
 alpha-galactosidase I, gel filtration Helianthus annuus
3.2.1.22 166000
-
 dormant seeds, alpha-galactosidase I, gel filtration Vicia sativa
3.2.1.22 167000
-
 alpha-galactosidase I, gel filtration Acer pseudoplatanus
3.2.1.22 195000
-
 alpha-galactosidase I, gel filtration Vicia dumetorum
3.2.1.22 209000
-
 alpha-galactosidase I, gel filtration Vigna radiata var. radiata
3.2.1.22 209000
-
 alpha-galactosidase I, gel filtration Vicia faba

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Acer pseudoplatanus
-
-
-
3.2.1.22 Aspergillus niger
-
-
-
3.2.1.22 Aspergillus paxillus
-
-
-
3.2.1.22 Bos taurus
-
-
-
3.2.1.22 Brassica oleracea
-
-
-
3.2.1.22 Cajanus indicus
-
-
-
3.2.1.22 Calvatia cyanthiformis
-
-
-
3.2.1.22 Canavalia ensiformis
-
-
-
3.2.1.22 Citrullus lanatus subsp. vulgaris
-
-
-
3.2.1.22 Coffea sp.
-
 green coffee beans
-
3.2.1.22 Cyamopsis tetragonoloba
-
-
-
3.2.1.22 Epidinium ecaudatum
-
-
-
3.2.1.22 Escherichia coli
-
-
-
3.2.1.22 Eudiplodinium maggii
-
-
-
3.2.1.22 Helianthus annuus
-
-
-
3.2.1.22 Helix pomatia
-
-
-
3.2.1.22 Hordeum sp.
-
-
-
3.2.1.22 Klebsiella aerogenes
-
-
-
3.2.1.22 Medicago sativa
-
-
-
3.2.1.22 Melolontha melolontha
-
-
-
3.2.1.22 Ophryoscolex caudatus
-
-
-
3.2.1.22 Phaseolus vulgaris
-
-
-
3.2.1.22 Pisum sativum
-
-
-
3.2.1.22 Plantago ovata
-
-
-
3.2.1.22 Polyplastron multivesiculatum
-
-
-
3.2.1.22 Prunus dulcis
-
-
-
3.2.1.22 Raphanus sativus
-
-
-
3.2.1.22 Rattus norvegicus
-
-
-
3.2.1.22 Ricinus communis
-
-
-
3.2.1.22 Saccharomyces sp.
-
 yeast
-
3.2.1.22 Spinacia oleracea
-
-
-
3.2.1.22 Streptococcus equinus
-
-
-
3.2.1.22 Streptococcus pneumoniae
-
-
-
3.2.1.22 Streptomyces olivaceus
-
-
-
3.2.1.22 Sus scrofa
-
-
-
3.2.1.22 Trigonella foenum-graecum
-
-
-
3.2.1.22 Triticum sp.
-
-
-
3.2.1.22 Tritrichomonas suis
-
-
-
3.2.1.22 Umbelopsis vinacea
-
-
-
3.2.1.22 Vicia dumetorum
-
-
-
3.2.1.22 Vicia faba
-
-
-
3.2.1.22 Vicia sativa
-
-
-
3.2.1.22 Vigna radiata var. radiata
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.22 glycoprotein
-
 Aspergillus niger
3.2.1.22 glycoprotein 2.7% D-glucosamine and 10.8% hexoses Umbelopsis vinacea
3.2.1.22 glycoprotein alpha-galactosidase I Vicia faba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.22 brain
-
 Rattus norvegicus
-
3.2.1.22 kidney
-
 Rattus norvegicus
-
3.2.1.22 liver
-
 Sus scrofa
-
3.2.1.22 liver
-
 Bos taurus
-
3.2.1.22 parathyroid gland
-
 Rattus norvegicus
-
3.2.1.22 thyroid gland
-
 Rattus norvegicus
-
3.2.1.22 uterus
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 1-naphthyl alpha-D-galactoside + H2O
-
 Saccharomyces sp. 1-naphthol + D-galactose
-
 ?
3.2.1.22 2-naphthyl alpha-D-galactoside + H2O
-
 Saccharomyces sp. 2-naphthol + D-glucose
-
 ?
3.2.1.22 6-bromo-2-naphthyl alpha-D-galactoside + H2O
-
 Vicia faba 6-bromo-2-naphthol + D-galactose
-
 ?
3.2.1.22 cellobiose + phenyl alpha-D-galactoside
-
 Coffea sp. ?
-
 ?
3.2.1.22 D-galactose + phenyl alpha-D-galactoside
-
 Phaseolus vulgaris ?
-
 ?
3.2.1.22 D-galactose + phenyl alpha-D-galactoside
-
 Vicia sativa ?
-
 ?
3.2.1.22 D-galactose + phenyl alpha-D-galactoside
-
 Ricinus communis ?
-
 ?
3.2.1.22 D-galactose + phenyl alpha-D-galactoside
-
 Raphanus sativus ?
-
 ?
3.2.1.22 D-galactose + phenyl alpha-D-galactoside
-
 Coffea sp. ?
-
 ?
3.2.1.22 D-galactose + phenyl alpha-D-galactoside
-
 Plantago ovata ?
-
 ?
3.2.1.22 D-glucose + melibiose
-
 Streptococcus pneumoniae melibiose + D-glucose
-
 ?
3.2.1.22 D-glucose + phenyl alpha-D-galactoside
-
 Vicia sativa ?
-
 ?
3.2.1.22 D-glucose + phenyl alpha-D-galactoside
-
 Trigonella foenum-graecum ?
-
 ?
3.2.1.22 D-glucose + phenyl alpha-D-galactoside
-
 Coffea sp. ?
-
 ?
3.2.1.22 D-glucose + phenyl alpha-D-galactoside
-
 Plantago ovata ?
-
 ?
3.2.1.22 D-glucose + raffinose
-
 Streptococcus pneumoniae ?
-
 ?
3.2.1.22 D-mannose + phenyl alpha-D-galactoside
-
 Vicia sativa epimelibiose + H2O
-
 ?
3.2.1.22 D-mannose + phenyl alpha-D-galactoside
-
 Coffea sp. epimelibiose + H2O
-
 ?
3.2.1.22 D-mannose + phenyl alpha-D-galactoside
-
 Plantago ovata epimelibiose + H2O
-
 ?
3.2.1.22 ethanol + phenyl alpha-D-galactoside
-
 Trigonella foenum-graecum ?
-
 ?
3.2.1.22 ethyl alpha-D-galactoside + H2O
-
 Vicia faba ethanol + D-galactose
-
 ?
3.2.1.22 ethyl alpha-D-galactoside + H2O
-
 Prunus dulcis ethanol + D-galactose
-
 ?
3.2.1.22 galactinol + H2O
-
 Vicia faba alpha-D-galactopyranose + myoinositol
-
 ?
3.2.1.22 gentianose + phenyl alpha-D-galactoside
-
 Coffea sp. ?
-
 ?
3.2.1.22 gentiobiose + phenyl alpha-D-galactoside
-
 Coffea sp. ?
-
 ?
3.2.1.22 glycerol + melibiose
-
 Coffea sp. floridoside + H2O
-
 ?
3.2.1.22 glycerol + phenyl alpha-D-galactoside
-
 Trigonella foenum-graecum ?
-
 ?
3.2.1.22 glycerol + phenyl alpha-D-galactoside
-
 Brassica oleracea ?
-
 ?
3.2.1.22 glycerol + phenyl alpha-D-galactoside
-
 Helianthus annuus ?
-
 ?
3.2.1.22 glycerol + phenyl alpha-D-galactoside
-
 Hordeum sp. ?
-
 ?
3.2.1.22 isopentanol + phenyl alpha-D-galactoside
-
 Trigonella foenum-graecum ?
-
 ?
3.2.1.22 isopropanol + phenyl alpha-D-galactoside
-
 Trigonella foenum-graecum ?
-
 ?
3.2.1.22 lactose + phenyl alpha-D-galactoside
-
 Coffea sp. ?
-
 ?
3.2.1.22 m-chlorophenyl alpha-D-galactoside + H2O
-
 Vicia faba m-chlorophenol + D-galactose
-
 ?
3.2.1.22 m-chlorophenyl alpha-D-galactoside + H2O
-
 Prunus dulcis m-chlorophenol + D-galactose
-
 ?
3.2.1.22 m-cresyl alpha-D-galactoside + H2O
-
 Vicia faba m-cresol + D-galactose
-
 ?
3.2.1.22 m-cresyl alpha-D-galactoside + H2O
-
 Prunus dulcis m-cresol + D-galactose
-
 ?
3.2.1.22 m-nitrophenyl alpha-D-galactoside + H2O
-
 Vicia faba m-nitrophenol + D-galactose
-
 ?
3.2.1.22 m-nitrophenyl alpha-D-galactoside + H2O
-
 Prunus dulcis m-nitrophenol + D-galactose
-
 ?
3.2.1.22 maltose + phenyl alpha-D-galactoside
-
 Coffea sp. ?
-
 ?
3.2.1.22 mannitol + phenyl alpha-D-galactoside
-
 Trigonella foenum-graecum ?
-
 ?
3.2.1.22 melibiose + H2O
-
 Vicia faba D-galactose + D-glucose
-
 ?
3.2.1.22 melibiose + H2O
-
 Vicia sativa D-galactose + D-glucose
-
 ?
3.2.1.22 melibiose + H2O
-
 Prunus dulcis D-galactose + D-glucose
-
 ?
3.2.1.22 melibiose + H2O
-
 Coffea sp. D-galactose + D-glucose
-
 ?
3.2.1.22 melibiose + H2O
-
 Saccharomyces sp. D-galactose + D-glucose
-
 ?
3.2.1.22 melibiose + H2O
-
 Umbelopsis vinacea D-galactose + D-glucose
-
 ?
3.2.1.22 melibiose + H2O
-
 Vicia dumetorum D-galactose + D-glucose
-
 ?
3.2.1.22 melibiose + melibiose
-
 Streptococcus pneumoniae ?
-
 ?
3.2.1.22 melibiose + melibiose
-
 Streptococcus equinus mannotriose + D-glucose
-
 ?
3.2.1.22 melibiose + methanol
-
 Coffea sp. methyl alpha-D-galactoside + D-glucose
-
 ?
3.2.1.22 meso-inositol + phenyl alpha-D-galactoside
-
 Trigonella foenum-graecum ?
-
 ?
3.2.1.22 methyl alpha-D-galactoside + H2O
-
 Vicia faba methanol + D-galactose
-
 ?
3.2.1.22 methyl alpha-D-galactoside + H2O
-
 Prunus dulcis methanol + D-galactose
-
 ?
3.2.1.22 N-acetyl-D-galactosamine + melibiose
-
 Tritrichomonas suis 6-O-alpha-D-galactosyl-N-acetyl-D-galactosamine + D-glucose
-
 ?
3.2.1.22 N-acetyl-D-glucosamine + melibiose
-
 Tritrichomonas suis 6-O-alpha-D-galactosyl-N-acetyl-D-glucosamine + H2O
-
 ?
3.2.1.22 n-butanol + phenyl alpha-D-galactoside
-
 Trigonella foenum-graecum ?
-
 ?
3.2.1.22 n-propanol + phenyl alpha-D-galactoside
-
 Trigonella foenum-graecum ?
-
 ?
3.2.1.22 n-propanol + phenyl alpha-D-galactoside
-
 Brassica oleracea ?
-
 ?
3.2.1.22 n-propanol + phenyl alpha-D-galactoside
-
 Helianthus annuus ?
-
 ?
3.2.1.22 n-propanol + phenyl alpha-D-galactoside
-
 Hordeum sp. ?
-
 ?
3.2.1.22 n-propyl alpha-D-galactoside + H2O
-
 Vicia faba n-propanol + D-galactose
-
 ?
3.2.1.22 n-propyl alpha-D-galactoside + H2O
-
 Prunus dulcis n-propanol + D-galactose
-
 ?
3.2.1.22 o-cresyl alpha-D-galactoside + H2O
-
 Vicia faba o-cresol + D-galactose
-
 ?
3.2.1.22 o-cresyl alpha-D-galactoside + H2O
-
 Prunus dulcis o-cresol + D-galactose
-
 ?
3.2.1.22 o-nitrophenyl alpha-D-galactoside + H2O
-
 Vicia faba o-nitrophenol + D-galactose
-
 ?
3.2.1.22 o-nitrophenyl alpha-D-galactoside + H2O
-
 Prunus dulcis o-nitrophenol + D-galactose
-
 ?
3.2.1.22 p-aminophenyl alpha-D-galactoside + H2O
-
 Vicia faba p-aminophenol + D-galactose
-
 ?
3.2.1.22 p-cresyl alpha-D-galactoside + H2O
-
 Vicia faba p-cresol + D-galactose
-
 ?
3.2.1.22 p-cresyl alpha-D-galactoside + H2O
-
 Prunus dulcis p-cresol + D-galactose
-
 ?
3.2.1.22 p-nitrophenyl alpha-D-fucoside + H2O
-
 Vicia faba p-nitrophenol + D-fucose
-
 ?
3.2.1.22 p-nitrophenyl beta-L-arabinoside + H2O
-
 Vicia faba p-nitrophenol + L-arabinose
-
 ?
3.2.1.22 p-nitrophenyl beta-L-arabinoside + H2O
-
 Prunus dulcis p-nitrophenol + L-arabinose
-
 ?
3.2.1.22 p-nitrophenyl-alpha-D-galactoside + H2O
-
 Vicia faba p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 p-nitrophenyl-alpha-D-galactoside + H2O
-
 Aspergillus niger p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 p-nitrophenyl-alpha-D-galactoside + H2O
-
 Medicago sativa p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 p-nitrophenyl-alpha-D-galactoside + H2O
-
 Prunus dulcis p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 phenyl alpha-D-galactoside + H2O
-
 Vicia faba phenol + D-galactose
-
 ?
3.2.1.22 phenyl alpha-D-galactoside + H2O
-
 Prunus dulcis phenol + D-galactose
-
 ?
3.2.1.22 phenyl alpha-D-galactoside + methanol
-
 Trigonella foenum-graecum methyl alpha-D-galactoside + phenol
-
 ?
3.2.1.22 phenyl alpha-D-galactoside + methanol
-
 Brassica oleracea methyl alpha-D-galactoside + phenol
-
 ?
3.2.1.22 phenyl alpha-D-galactoside + methanol
-
 Helianthus annuus methyl alpha-D-galactoside + phenol
-
 ?
3.2.1.22 phenyl alpha-D-galactoside + methanol
-
 Coffea sp. methyl alpha-D-galactoside + phenol
-
 ?
3.2.1.22 phenyl alpha-D-galactoside + methanol
-
 Hordeum sp. methyl alpha-D-galactoside + phenol
-
 ?
3.2.1.22 raffinose + galactinol
-
 Phaseolus vulgaris stachyose + H2O
-
 ?
3.2.1.22 raffinose + H2O
-
 Vicia faba sucrose + D-galactose
-
 ?
3.2.1.22 raffinose + H2O
-
 Vicia sativa sucrose + D-galactose
-
 ?
3.2.1.22 raffinose + H2O
-
 Escherichia coli sucrose + D-galactose
-
 ?
3.2.1.22 raffinose + H2O
-
 Aspergillus niger sucrose + D-galactose
-
 ?
3.2.1.22 raffinose + H2O
-
 Medicago sativa sucrose + D-galactose
-
 ?
3.2.1.22 raffinose + H2O
-
 Prunus dulcis sucrose + D-galactose
-
 ?
3.2.1.22 raffinose + H2O
-
 Coffea sp. sucrose + D-galactose
-
 ?
3.2.1.22 raffinose + H2O
-
 Umbelopsis vinacea sucrose + D-galactose
-
 ?
3.2.1.22 raffinose + H2O
-
 Vicia dumetorum sucrose + D-galactose
-
 ?
3.2.1.22 raffinose + melibiose
-
 Streptococcus equinus stachyose + H2O
-
 ?
3.2.1.22 raffinose + methanol
-
 Coffea sp. methyl alpha-D-galactoside + verbascose + ajugose
-
 ?
3.2.1.22 raffinose + raffinose
-
 Streptococcus pneumoniae ?
-
 ?
3.2.1.22 sorbitol + phenyl alpha-D-galactoside
-
 Trigonella foenum-graecum ?
-
 ?
3.2.1.22 stachyose + H2O
-
 Vicia faba ?
-
 ?
3.2.1.22 stachyose + H2O
-
 Escherichia coli ?
-
 ?
3.2.1.22 stachyose + H2O
-
 Coffea sp. ?
-
 ?
3.2.1.22 stachyose + H2O
-
 Umbelopsis vinacea ?
-
 ?
3.2.1.22 stachyose + methanol
-
 Coffea sp. methyl alpha-D-galactoside + verbascose + ajugose
-
 ?
3.2.1.22 stachyose + stachyose
-
 Streptococcus pneumoniae ?
-
 ?
3.2.1.22 sucrose + galactinol
-
 Phaseolus vulgaris raffinose + H2O
-
 ?
3.2.1.22 sucrose + melibiose
-
 Coffea sp. raffinose + stachyose + planteose
-
 ?
3.2.1.22 sucrose + melibiose
-
 Streptococcus equinus raffinose + D-glucose
-
 ?
3.2.1.22 sucrose + phenyl alpha-D-galactoside
-
 Coffea sp. raffinose + stachyose + planteose
-
 ?
3.2.1.22 sucrose + phenyl alpha-D-galactoside
-
 Vicia sativa ?
-
 ?
3.2.1.22 sucrose + phenyl alpha-D-galactoside
-
 Trigonella foenum-graecum ?
-
 ?
3.2.1.22 sucrose + phenyl alpha-D-galactoside
-
 Plantago ovata ?
-
 ?
3.2.1.22 sucrose + raffinose
-
 Triticum sp. planteose + D-glucose
-
 ?
3.2.1.22 trehalose + phenyl alpha-D-galactoside
-
 Trigonella foenum-graecum ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 43
-
 10 min, 50% loss in activity Spinacia oleracea
3.2.1.22 50
-
 60 min, 30% loss in activity Streptococcus pneumoniae
3.2.1.22 55
-
 15 min, 90% loss of activity Streptomyces olivaceus
3.2.1.22 55
-
 60 min, 35% loss in activity Aspergillus niger
3.2.1.22 55
-
 5 min, 20% loss in activity Bos taurus
3.2.1.22 60
-
 5 min, complete loss in activity Bos taurus
3.2.1.22 60
-
 20 min, 15% loss of activity Prunus dulcis
3.2.1.22 60
-
 alpha-galactosidase I, 30 min, 42% loss in activity, alpha-galactosidase II, 30 min, 81% loss of activity Vicia faba
3.2.1.22 62
-
 5 min, 20% loss in activity Canavalia ensiformis
3.2.1.22 70
-
 30 min, complete loss in activity Helix pomatia
3.2.1.22 75
-
 40 min, 16% loss in activity Vicia sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 2.5 3.5 alpha-galactosidase II, p-nitrophenyl alpha-D-galactoside as substrate Vicia faba
3.2.1.22 3 3.5 alpha-galactosidase II Pisum sativum
3.2.1.22 3 3.5 alpha-galactosidase I, p-nitrophenyl alpha-D-galactoside as substrate Vicia faba
3.2.1.22 3 5 o-nitrophenyl alpha-D-galactoside as substrate Calvatia cyanthiformis
3.2.1.22 3 5 raffinose as substrate Umbelopsis vinacea
3.2.1.22 3 6.5 alpha-galactosidase I Pisum sativum
3.2.1.22 3.2
-
-
 Trigonella foenum-graecum
3.2.1.22 3.2 3.8
-
 Helix pomatia
3.2.1.22 3.5 4.5 1-naphthyl alpha-D-galactoside, 2-naphthyl alpha-D-galactoside or 6-bromo-2-naphthyl alpha-D-galactoside as substrate Saccharomyces sp.
3.2.1.22 3.5 4.5 alpha-galactosidase II, raffinose as substrate Vicia faba
3.2.1.22 3.5 5.5 alpha-galactosidase I, raffinose as substrate Vicia faba
3.2.1.22 3.5 5.3 melibiose as substrate Saccharomyces sp.
3.2.1.22 3.6 4.2
-
 Coffea sp.
3.2.1.22 3.8 4.5 p-nitrophenyl alpha-D-galactoside as substrate Aspergillus niger
3.2.1.22 4 5 p-nitrophenyl alpha-D-galactoside as substrate Canavalia ensiformis
3.2.1.22 4 6
-
 Streptomyces olivaceus
3.2.1.22 4 6 stachyose or o-nitrophenyl alpha-D-galactoside as substrate Umbelopsis vinacea
3.2.1.22 4 4.5 kidney Rattus norvegicus
3.2.1.22 4.2
-
-
 Citrullus lanatus subsp. vulgaris
3.2.1.22 4.2 4.8 melibiitol as substrate Aspergillus niger
3.2.1.22 4.6
-
-
 Trigonella foenum-graecum
3.2.1.22 4.6
-
 phenyl alpha-D-galactoside as substrate Aspergillus paxillus
3.2.1.22 4.9
-
 brain Rattus norvegicus
3.2.1.22 5 5.5
-
 Epidinium ecaudatum
3.2.1.22 5 5.5 alpha-galactosidase II, p-nitrophenyl alpha-D-galactoside as substrate Vicia faba
3.2.1.22 5 5.5 alpha-galactosidase II Pisum sativum
3.2.1.22 5.1
-
-
 Eudiplodinium maggii
3.2.1.22 5.2
-
-
 Sus scrofa
3.2.1.22 5.2
-
 uterus Rattus norvegicus
3.2.1.22 5.3
-
-
 Spinacia oleracea
3.2.1.22 5.3
-
-
 Polyplastron multivesiculatum
3.2.1.22 5.3
-
 alpha-galactosidase II Plantago ovata
3.2.1.22 5.3
-
 alpha-galactosidase I, phenyl alpha-D-galactoside as substrate Coffea sp.
3.2.1.22 5.4
-
-
 Streptomyces olivaceus
3.2.1.22 5.5 5.7
-
 Prunus dulcis
3.2.1.22 5.6
-
-
 Ophryoscolex caudatus
3.2.1.22 5.6 6
-
 Streptococcus pneumoniae
3.2.1.22 5.6 6.3
-
 Streptococcus equinus
3.2.1.22 5.9
-
 alpha-galactosidase I Plantago ovata
3.2.1.22 6 6.5 alpha-galactosidase I, p-nitrophenyl alpha-D-galactoside as substrate Vicia faba
3.2.1.22 6
-
-
 Melolontha melolontha
3.2.1.22 6
-
 alpha-galactosidase II, phenyl alpha-D-galactoside as substrate Coffea sp.
3.2.1.22 6.3
-
-
 Vicia sativa
3.2.1.22 6.3
-
-
 Coffea sp.
3.2.1.22 6.5 6.7
-
 Phaseolus vulgaris
3.2.1.22 7.5
-
-
 Escherichia coli