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Literature summary extracted from

  • White, K.N.; Hope, D.B.
    Partial purification and characterization of a microsomal carboxylesterase specific for salicylate esters from guinea-pig liver (1984), Biochim. Biophys. Acta, 785, 138-147.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 bis(4-nitrophenyl) phosphate
-
 Cavia porcellus
3.1.1.1 additional information substrate inhibition increases with increasing chain length of the fatty acid on the ester Cavia porcellus
3.1.1.55 bis(4-nitrophenyl)hydrogen phosphate irreversible, complete inhibition Cavia porcellus
3.1.1.55 bis-4-nitrophenyl phosphate
-
 Cavia porcellus
3.1.1.55 additional information substrate inhibition increases with increasing chain length of the fatty acid on the ester Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.132
-
 n-Decanoyl salicylate
-
 Cavia porcellus
3.1.1.1 0.21
-
 n-Octanoyl salicylate
-
 Cavia porcellus
3.1.1.1 0.447
-
 aspirin
-
 Cavia porcellus
3.1.1.1 0.576
-
 n-Propionyl salicylate
-
 Cavia porcellus
3.1.1.1 0.679
-
 n-Hexanoyl salicylate
-
 Cavia porcellus
3.1.1.1 0.799
-
 n-Butyryl salicylate
-
 Cavia porcellus
3.1.1.1 0.983
-
 n-valeryl salicylate
-
 Cavia porcellus
3.1.1.55 0.132
-
 n-Decanoyl salicylate pH 8.0, 37°C Cavia porcellus
3.1.1.55 0.201
-
 n-Octanoyl salicylate pH 8.0, 37°C Cavia porcellus
3.1.1.55 0.447
-
 Acetyl salicylate pH 8.0, 37°C Cavia porcellus
3.1.1.55 0.576
-
 n-Propionyl salicylate pH 8.0, 37°C Cavia porcellus
3.1.1.55 0.679
-
 n-Hexanoyl salicylate pH 8.0, 37°C Cavia porcellus
3.1.1.55 0.799
-
 n-Butyryl salicylate pH 8.0, 37°C Cavia porcellus
3.1.1.55 0.983
-
 n-Pentanoyl salicylate pH 8.0, 37°C Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.1 microsome
-
 Cavia porcellus
-
-
3.1.1.55 microsome
-
 Cavia porcellus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 54300
-
 gel filtration Cavia porcellus
3.1.1.1 57100
-
 1 * 57100, SDS-PAGE Cavia porcellus
3.1.1.55 54300
-
 gel filtration Cavia porcellus
3.1.1.55 57100
-
 1 * 57100, SDS-PAGE Cavia porcellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.55 acetyl salicylate + H2O Cavia porcellus i.e. aspirin salicylate + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Cavia porcellus
-
-
-
3.1.1.55 Cavia porcellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 aspirin hydrolase, partial Cavia porcellus
3.1.1.55 partial Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.1 liver
-
 Cavia porcellus
-
3.1.1.55 liver
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.55 additional information
-
-
 Cavia porcellus
3.1.1.55 11.31
-
 partially purified enzyme, substrate acetyl salicylate Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 aspirin + H2O
-
 Cavia porcellus acetate + salicylate
-
 ir
3.1.1.1 cholesterol oleate + H2O
-
 Cavia porcellus cholesterol + oleate
-
 ?
3.1.1.1 n-butyryl salicylate + H2O
-
 Cavia porcellus n-butanol + salicylate
-
 ?
3.1.1.1 n-decanoyl salicylate + H2O
-
 Cavia porcellus ? + salicylate
-
 ?
3.1.1.1 n-hexanoyl salicylate + H2O
-
 Cavia porcellus n-hexanol + salicylate
-
 ?
3.1.1.1 n-octanoyl salicylate + H2O
-
 Cavia porcellus ? + salicylate
-
 ?
3.1.1.1 n-propionyl salicylate + H2O
-
 Cavia porcellus n-propionate + salicylate
-
 ?
3.1.1.1 n-propyl salicylate + H2O
-
 Cavia porcellus n-propanol + salicylate
-
 ?
3.1.1.1 n-valeryl salicylate + H2O
-
 Cavia porcellus n-pentanol + salicylate
-
 ?
3.1.1.1 triolein + H2O
-
 Cavia porcellus oleic acid + glycerol
-
 ?
3.1.1.55 acetyl salicylate + H2O i.e. aspirin Cavia porcellus salicylate + acetate
-
 ?
3.1.1.55 acetylsalicylate + H2O i.e. aspirin Cavia porcellus salicylate + acetate
-
 ?
3.1.1.55 n-butyryl salicylate + H2O
-
 Cavia porcellus 2-hydroxybenzoate + butyrate
-
 ?
3.1.1.55 n-decanoyl salicylate + H2O
-
 Cavia porcellus 2-hydroxybenzoate + n-decanoate
-
 ?
3.1.1.55 n-hexanoyl salicylate + H2O
-
 Cavia porcellus 2-hydroxybenzoate + n-hexanoate
-
 ?
3.1.1.55 n-octanoyl salicylate + H2O
-
 Cavia porcellus 2-hydroxybenzoate + n-octanoate
-
 ?
3.1.1.55 n-pentanoyl salicylate + H2O
-
 Cavia porcellus 2-hydroxybenzoate + n-pentanoate
-
 ?
3.1.1.55 n-propionyl salicylate + H2O
-
 Cavia porcellus 2-hydroxybenzoate + propionate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 monomer 1 * 57100, SDS-PAGE Cavia porcellus
3.1.1.55 monomer 1 * 57100, SDS-PAGE Cavia porcellus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 aspirin hydrolase
-
 Cavia porcellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.55 37
-
 assay at Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 7 8.5
-
 Cavia porcellus
3.1.1.55 7 8.5
-
 Cavia porcellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 5.5 9 pH 5.5: about 55% of maximal activity, pH 9.0: about 80% of maximal activity Cavia porcellus
3.1.1.55 5.5 9 pH 5.5: about 50% of activity maximum, pH 9: about 75% of activity maximum Cavia porcellus