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Literature summary extracted from

  • Shailubhai, K.; Pratta, M.A.; Vijay, I.K.
    Purification and characterization of glucosidase I involved in N-linked glycoprotein processing in bovine mammary gland (1987), Biochem. J., 247, 555-562.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.106 Lubrol Px activation Rattus norvegicus
3.2.1.106 Lubrol Px activation Bos taurus
3.2.1.106 additional information no significant activation by phospholipids Bos taurus
3.2.1.106 Triton X-100
-
 Rattus norvegicus
3.2.1.106 Triton X-100
-
 Bos taurus

General Stability

EC Number General Stability Organism
3.2.1.106 1% poly(ethylene glycol) 4000 stabilizes Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.106 1-deoxynojirimycin strong inhibition Bos taurus
3.2.1.106 1-deoxynojirimycin
-
 Mus musculus
3.2.1.106 1-deoxynojirimycin
-
 Rattus norvegicus
3.2.1.106 Ag+ potent inhibitor, inhibition reversed by adding an excess of dithiothreitol Bos taurus
3.2.1.106 Co2+ potent inhibitor, inhibition reversed by adding an excess of dithiothreitol Bos taurus
3.2.1.106 Cu2+ potent inhibitor, inhibition reversed by adding an excess of dithiothreitol Bos taurus
3.2.1.106 D-glucose no significant inhibition, 10 mM: 13% inhibition Bos taurus
3.2.1.106 D-mannose 10 mM: no inhibition Bos taurus
3.2.1.106 heavy-metal ions potent inhibitors, inhibition reversed by adding an excess of dithiothreitol Bos taurus
3.2.1.106 Hg2+ potent inhibitor, inhibition reversed by adding an excess of dithiothreitol Bos taurus
3.2.1.106 kojibiose 1 mM: 76% inhibition; alpha-1,2-linked glucose disaccharide Bos taurus
3.2.1.106 additional information no inhibition by alpha,alpha'-bipyridyl and o-phenanthroline; no inhibition by EDTA; no significant inhibition by nigerose, Glc(alpha1-3)Glc disaccharide, maltose, gentibiose, cellobiose, sucrose Bos taurus
3.2.1.106 additional information
-
 Mus musculus
3.2.1.106 additional information no inhibition by EDTA Rattus norvegicus
3.2.1.106 N-(5-carboxypentyl)-1-deoxynojirimycin
-
 Bos taurus
3.2.1.106 p-nitrophenyl-alpha-D-glucoside 0.0005 mM: 19% inhibition Bos taurus
3.2.1.106 p-nitrophenyl-alpha-D-glucoside
-
 Rattus norvegicus
3.2.1.106 p-nitrophenyl-beta-D-glucoside 0.0005 mM: 14% inhibition Bos taurus
3.2.1.106 p-nitrophenyl-beta-D-glucoside
-
 Rattus norvegicus
3.2.1.106 Zn2+ potent inhibitor, inhibition reversed by adding an excess of dithiothreitol Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.106 endoplasmic reticulum
-
 Rattus norvegicus 5783
-
3.2.1.106 endoplasmic reticulum
-
 Bos taurus 5783
-
3.2.1.106 microsome
-
 Rattus norvegicus
-
-
3.2.1.106 microsome
-
 Bos taurus
-
-
3.2.1.106 additional information
-
 Rattus norvegicus
-
-
3.2.1.106 additional information specific antibodies against glucosidase I allow subcellular localization of glucosidase I Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.106 additional information
-
 Rattus norvegicus
3.2.1.106 additional information metal ions not required Bos taurus
3.2.1.106 phosphate activation up to 25 mM Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.106 85000
-
 4 * 85000, SDS-PAGE reducing conditions Bos taurus
3.2.1.106 320000 330000 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.106 Glc3Man9GlcNAc2 + H2O Cavia porcellus
-
 ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Rattus norvegicus hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Bos taurus hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Rattus norvegicus specific involvement in the N-linked oligosaccharide-processing pathway ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Bos taurus specific involvement in the N-linked oligosaccharide-processing pathway ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Mus musculus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Rattus norvegicus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Bos taurus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
3.2.1.106 additional information Mus musculus catalyzes initial step in post-glycosylation processing of cellular glycoprotein ?
-
 ?
3.2.1.106 additional information Rattus norvegicus catalyzes initial step in post-glycosylation processing of cellular glycoprotein ?
-
 ?
3.2.1.106 additional information Bos taurus catalyzes initial step in post-glycosylation processing of cellular glycoprotein ?
-
 ?
3.2.1.106 additional information Mus musculus involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
3.2.1.106 additional information Rattus norvegicus involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
3.2.1.106 additional information Bos taurus involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
3.2.1.106 additional information Mus musculus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
3.2.1.106 additional information Rattus norvegicus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
3.2.1.106 additional information Bos taurus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
3.2.1.106 additional information Bos taurus glucosidase I is a control enzyme for the regulation of glycoprotein processing ?
-
 ?
3.2.1.106 additional information Bos taurus play a crucial role in the overall regulation of glycoprotein biosynthesis ?
-
 ?
3.2.1.106 additional information Mus musculus involved in the formation of high mannose and complex glycoproteins ?
-
 ?
3.2.1.106 additional information Bos taurus involved in the formation of high mannose and complex glycoproteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.106 Bos taurus
-
 cow
-
3.2.1.106 Bos taurus
-
 lactating cow
-
3.2.1.106 Cavia porcellus
-
-
-
3.2.1.106 Mus musculus
-
-
-
3.2.1.106 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.106 lactating mammary gland Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.106 heart
-
 Cavia porcellus
-
3.2.1.106 heart
-
 Bos taurus
-
3.2.1.106 liver
-
 Cavia porcellus
-
3.2.1.106 liver
-
 Mus musculus
-
3.2.1.106 liver
-
 Rattus norvegicus
-
3.2.1.106 liver
-
 Bos taurus
-
3.2.1.106 mammary gland
-
 Cavia porcellus
-
3.2.1.106 mammary gland
-
 Mus musculus
-
3.2.1.106 mammary gland
-
 Rattus norvegicus
-
3.2.1.106 mammary gland lactating mammary glands Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.106 additional information
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.2.1.106 -20°C, several months, without loss of activity Bos taurus
3.2.1.106 25°C, buffer containing 1% poly(ethylene glycol) 4000, 72 h, 75% activity retained Bos taurus
3.2.1.106 4°C, 1 week, without loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.106 Glc3Man9GlcNAc2 + H2O
-
 Cavia porcellus D-glucose + Glc2Man9GlcNAc2
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O
-
 Mus musculus D-glucose + Glc2Man9GlcNAc2
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Rattus norvegicus D-glucose + Glc2Man9GlcNAc2
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Bos taurus D-glucose + Glc2Man9GlcNAc2
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O
-
 Cavia porcellus ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Rattus norvegicus ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Bos taurus ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Rattus norvegicus ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Bos taurus ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Mus musculus ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Rattus norvegicus ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Bos taurus ?
-
 ?
3.2.1.106 additional information Glc1Man9GlcNAc2: not a substrate Bos taurus ?
-
 ?
3.2.1.106 additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Rattus norvegicus ?
-
 ?
3.2.1.106 additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Bos taurus ?
-
 ?
3.2.1.106 additional information p-nitrophenyl-alpha-D-glucopyranoside: not a substrate Bos taurus ?
-
 ?
3.2.1.106 additional information Glc2Man9GlcNAc2: not a substrate Bos taurus ?
-
 ?
3.2.1.106 additional information p-nitrophenyl-beta-D-glucopyranoside: not a substrate Bos taurus ?
-
 ?
3.2.1.106 additional information catalyzes initial step in post-glycosylation processing of cellular glycoprotein Mus musculus ?
-
 ?
3.2.1.106 additional information catalyzes initial step in post-glycosylation processing of cellular glycoprotein Rattus norvegicus ?
-
 ?
3.2.1.106 additional information catalyzes initial step in post-glycosylation processing of cellular glycoprotein Bos taurus ?
-
 ?
3.2.1.106 additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Mus musculus ?
-
 ?
3.2.1.106 additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Rattus norvegicus ?
-
 ?
3.2.1.106 additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Bos taurus ?
-
 ?
3.2.1.106 additional information involved in processing of asparagine-linked oligosaccharides Mus musculus ?
-
 ?
3.2.1.106 additional information involved in processing of asparagine-linked oligosaccharides Rattus norvegicus ?
-
 ?
3.2.1.106 additional information involved in processing of asparagine-linked oligosaccharides Bos taurus ?
-
 ?
3.2.1.106 additional information glucosidase I is a control enzyme for the regulation of glycoprotein processing Bos taurus ?
-
 ?
3.2.1.106 additional information play a crucial role in the overall regulation of glycoprotein biosynthesis Bos taurus ?
-
 ?
3.2.1.106 additional information involved in the formation of high mannose and complex glycoproteins Mus musculus ?
-
 ?
3.2.1.106 additional information involved in the formation of high mannose and complex glycoproteins Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.106 tetramer 4 * 85000, SDS-PAGE reducing conditions Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.106 37
-
-
 Rattus norvegicus
3.2.1.106 37
-
 assay at Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.106 25
-
 50% activity lost after 24 h, in buffer containing 1% poly(ethylene glycol) 4000: 75% activity retained after 72 h Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.106 6.6 7
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.106 6 7.5 half-maximal activity at Bos taurus