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Literature summary extracted from

  • Lea, P.J.; Fowden, L.
    The purification and properties of glutamine-dependent asparagine synthetase isolated from Lupinus luteus (1975), Proc. R. Soc. Lond. B Biol. Sci., 192, 13-26.
No PubMed abstract available

General Stability

EC Number General Stability Organism
6.3.5.4 glycerol and 2-mercaptoethanol are essential for enzyme stability during purification at 4°C Lupinus albus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.4 2-Amino-2-carboxy-L-ethanesulfonamide
-
 Lupinus albus
6.3.5.4 2-Hydroxyethyl-L-Gln
-
 Lupinus albus
6.3.5.4 2-oxoglutarate in presence of aminoxyacetate, 50% inhibition at 5 mM Lupinus albus
6.3.5.4 5-Bromo-4-oxo-L-norvaline
-
 Lupinus albus
6.3.5.4 5-Chloro-4-oxo-L-norvaline
-
 Lupinus albus
6.3.5.4 Albizzine
-
 Lupinus albus
6.3.5.4 AMP 2.5 mM, 50% inhibition Lupinus albus
6.3.5.4 Asn
-
 Lupinus albus
6.3.5.4 ATP strong inhibition above 5 mM Lupinus albus
6.3.5.4 azaserine
-
 Lupinus albus
6.3.5.4 beta-Methyl-DL-Asn
-
 Lupinus albus
6.3.5.4 Ca2+
-
 Lupinus albus
6.3.5.4 Cu2+
-
 Lupinus albus
6.3.5.4 diphosphate 5 mM, 45% inhibition Lupinus albus
6.3.5.4 DL-homo-Gln
-
 Lupinus albus
6.3.5.4 erythro-beta-Hydroxy-L-Asn
-
 Lupinus albus
6.3.5.4 erythro-beta-hydroxy-L-Asp
-
 Lupinus albus
6.3.5.4 erythro-beta-Methyl-L-Asp
-
 Lupinus albus
6.3.5.4 gamma-Methylene-L-Gln
-
 Lupinus albus
6.3.5.4 Gln D-Gln, weak Lupinus albus
6.3.5.4 Glu 150 mM, 50% inhibition Lupinus albus
6.3.5.4 L-Glutamate diamide
-
 Lupinus albus
6.3.5.4 L-Glutamate-gamma-ethyl ester
-
 Lupinus albus
6.3.5.4 L-glutamate-gamma-methyl ester
-
 Lupinus albus
6.3.5.4 L-methionine-S-sulfoximine
-
 Lupinus albus
6.3.5.4 Li+
-
 Lupinus albus
6.3.5.4 meso-Diaminosuccinamate
-
 Lupinus albus
6.3.5.4 N-acetyl-L-Gln
-
 Lupinus albus
6.3.5.4 N-alpha-Methyl-L-Asn
-
 Lupinus albus
6.3.5.4 N-Carbobenzoxy-DL-Gln
-
 Lupinus albus
6.3.5.4 N-ethyl-L-Asn
-
 Lupinus albus
6.3.5.4 N-Methyl-L-Asn
-
 Lupinus albus
6.3.5.4 S-Carbamoylcysteine
-
 Lupinus albus
6.3.5.4 Sn2+
-
 Lupinus albus
6.3.5.4 Sr2+
-
 Lupinus albus
6.3.5.4 threo-beta-Hydroxy-L-Asn
-
 Lupinus albus
6.3.5.4 threo-beta-methyl-L-Asp
-
 Lupinus albus
6.3.5.4 Zn2+
-
 Lupinus albus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.4 0.04
-
 L-Gln
-
 Lupinus albus
6.3.5.4 0.15
-
 ATP
-
 Lupinus albus
6.3.5.4 0.8
-
 L-Asp
-
 Lupinus albus
6.3.5.4 2.1
-
 NH4+
-
 Lupinus albus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.5.4 cytosol
-
 Lupinus albus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.4 Co2+ can partially replace Mg2+ in activation Lupinus albus
6.3.5.4 Mg2+ Km: 1.1 mM Lupinus albus
6.3.5.4 Mn2+ can partially substitute for Mg2+ Lupinus albus
6.3.5.4 Ni2+ can partially replace Mg2+ in activation Lupinus albus

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.4 Lupinus albus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.5.4 cotyledon
-
 Lupinus albus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.5.4 0.193
-
-
 Lupinus albus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.4 ATP + L-Asp + L-Gln
-
 Lupinus albus AMP + diphosphate + Asn + Glu
-
 ?
6.3.5.4 ATP + L-Asp + NH3 30% of the activity relative to Gln Lupinus albus AMP + diphosphate + Asn
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.4 7.9 8.3
-
 Lupinus albus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.5.4 2.5
-
 AMP
-
 Lupinus albus
6.3.5.4 150
-
 Glu
-
 Lupinus albus