EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.3.11 | D68E | mutation shifts the pH-optimum from pH 7.0 for the wild type enzyme to pH 8.5 for the mutant enzyme, decreased binding affinity for Mg2+ compared to wild type enzyme | Sus scrofa |
3.1.3.11 | D74A | 50000fold reduced turnover number relative to wild type enzyme | Sus scrofa |
3.1.3.11 | D74E | mutation shifts the pH-optimum from pH 7.0 for the wild type enzyme to pH 8.5 for the mutant enzyme, decreased binding affinity for Mg2+ compared to wild type enzyme, no AMP cooperativity, kinetic mechanism of AMP inhibition with respect to Mg2+ is changed from competitive to noncompetitive | Sus scrofa |
3.1.3.11 | D74N | 2000fold reduced turnover number relative to wild type enzyme | Sus scrofa |
3.1.3.11 | K71A | mutation shifts the pH-optimum from pH 7.0 for the wild type enzyme to pH 7.5 for the mutant enzyme | Sus scrofa |
3.1.3.11 | K71M/K72M | mutation shifts the pH-optimum from pH 7.0 for the wild type enzyme to pH 7.5 for the mutant enzyme, 175fold increased inhibition constant for AMP, 2fold increased affinity for Mg2+ | Sus scrofa |
3.1.3.11 | N64A | mutation shifts the pH-optimum from pH 7.0 for the wild type enzyme to pH 8.5 for the mutant enzyme, decreased binding affinity for Mg2+ compared to wild type enzyme, no AMP cooperativity, kinetic mechanism of AMP inhibition with respect to Mg2+ is changed from competitive to noncompetitive | Sus scrofa |
3.1.3.11 | N64Q | mutation shifts the pH-optimum from pH 7.0 for the wild type enzyme to pH 8.5 for the mutant enzyme, decreased binding affinity for Mg2+ compared to wild type enzyme | Sus scrofa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.11 | 0.00103 | - |
fructose 1,6-diphosphate | mutant enzyme D74E, pH 8.5 | Sus scrofa | |
3.1.3.11 | 0.00131 | - |
fructose 1,6-diphosphate | mutant enzyme D68E, pH 7.5 | Sus scrofa | |
3.1.3.11 | 0.00142 | - |
fructose 1,6-diphosphate | mutant enzyme N64A, pH 8.5 | Sus scrofa | |
3.1.3.11 | 0.0016 | - |
fructose 1,6-diphosphate | mutant enzyme K71M/K72M, pH 7.5 | Sus scrofa | |
3.1.3.11 | 0.0017 | - |
fructose 1,6-diphosphate | mutant enzyme N64E, pH 8.5 | Sus scrofa | |
3.1.3.11 | 0.0022 | - |
fructose 1,6-diphosphate | wild type enzyme, pH 8.5 | Sus scrofa | |
3.1.3.11 | 0.0028 | - |
fructose 1,6-diphosphate | wild type enzyme, pH 7.5 | Sus scrofa | |
3.1.3.11 | 0.0028 | - |
fructose 1,6-diphosphate | mutant enzyme K71A, pH 7.5 | Sus scrofa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.11 | Sus scrofa | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.11 | D-fructose 1,6-diphosphate + H2O | - |
Sus scrofa | D-fructose 6-phosphate + phosphate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.11 | 0.0004 | - |
fructose 1,6-diphosphate | mutant enzyme D74A, pH 8.5 | Sus scrofa | |
3.1.3.11 | 0.01 | - |
fructose 1,6-diphosphate | mutant enzyme D74N, pH 8.5 | Sus scrofa | |
3.1.3.11 | 1.2 | - |
fructose 1,6-diphosphate | mutant enzyme D74E, pH 8.5 | Sus scrofa | |
3.1.3.11 | 5.4 | - |
fructose 1,6-diphosphate | mutant enzyme N64E, pH 8.5 | Sus scrofa | |
3.1.3.11 | 6.4 | - |
fructose 1,6-diphosphate | mutant enzyme D68E, pH 7.5 | Sus scrofa | |
3.1.3.11 | 7.1 | - |
fructose 1,6-diphosphate | mutant enzyme N64A, pH 8.5 | Sus scrofa | |
3.1.3.11 | 9.1 | - |
fructose 1,6-diphosphate | wild type enzyme, pH 8.5 | Sus scrofa | |
3.1.3.11 | 16 | - |
fructose 1,6-diphosphate | mutant enzyme K71A, pH 7.5 | Sus scrofa | |
3.1.3.11 | 21 | - |
fructose 1,6-diphosphate | wild type enzyme, pH 7.5 | Sus scrofa | |
3.1.3.11 | 34 | - |
fructose 1,6-diphosphate | mutant enzyme K71M/K72M, pH 7.5 | Sus scrofa |