Any feedback?
Literature summary extracted from
- Fructose-1,6-diphosphate from bovine liver (1975), Methods Enzymol., 42C, 363-368.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.11 | 1-Fluoro-2,4-dinitrobenzene | up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus |  |
| 3.1.3.11 | 5,5'-dithiobis-(2-nitrobenzoate) | up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
| 3.1.3.11 | nagarse | stimulates 2fold to 3fold at pH 9.0, loss of activity at pH 6.5 | Bos taurus | |
| 3.1.3.11 | NEM | up to 90% inhibition at pH 6.5-7.0, slight increase of activity at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
| 3.1.3.11 | p-mercuribenzoate | up to 90% inhibition at pH 6.5-7.0, slight increase of activity at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
| 3.1.3.11 | papain | stimulates 2fold to 3fold at pH 9.0, loss of activity at pH 6.5 | Bos taurus | |
| 3.1.3.11 | Pronase | stimulates 2fold to 3fold at pH 9.0, loss of activity at pH 6.5 | Bos taurus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.11 | 1-Fluoro-2,4-dinitrobenzene | up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
| 3.1.3.11 | 5,5'-dithiobis(2-nitrobenzoate) | up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
| 3.1.3.11 | AMP | allosteric | Bos taurus | |
| 3.1.3.11 | fructose 1,6-diphosphate | at high concentrations | Bos taurus | |
| 3.1.3.11 | Glutaraldehyde | - |
Bos taurus | |
| 3.1.3.11 | iodoacetate | - |
Bos taurus | |
| 3.1.3.11 | nagarse | stimulates 2fold to 3fold at pH 9.0, loss of activity at pH 6.5 | Bos taurus | |
| 3.1.3.11 | NEM | up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
| 3.1.3.11 | p-mercuribenzoate | up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
| 3.1.3.11 | papain | stimulates 2fold to 3fold at pH 9.0, loss of activity at pH 6.5 | Bos taurus | |
| 3.1.3.11 | Pronase | stimulates 2fold to 3fold at pH 9.0, loss of activity at pH 6.5 | Bos taurus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.11 | 0.0017 | - |
fructose 1,6-diphosphate | - |
Bos taurus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.11 | Mn2+ | divalent cation required. Mn2+ or Mg2+ | Bos taurus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.11 | 130000 | - |
equilibrium sedimentation | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.11 | D-fructose 1,6-diphosphate + H2O | Bos taurus | enzyme in gluconeogenic pathway | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.11 | Bos taurus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.3.11 | liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.11 | 18 | - |
- |
Bos taurus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.3.11 | -20°C, pH 7.5-8.0, 10 mM Tris, 1.0 mM EDTA, 3.0 mM MgSO4 or 20 mM beta-glycerophosphate, 1.0 mM fructose 1,6-diphosphate, stable for many months | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.11 | beta-glycerophosphate + H2O | substrate at pH 9.0, no activity at pH 6.5 | Bos taurus | ? | - |
? | |
| 3.1.3.11 | D-fructose 1,6-diphosphate + H2O | enzyme in gluconeogenic pathway | Bos taurus | ? | - |
? | |
| 3.1.3.11 | D-fructose 1,6-diphosphate + H2O | - |
Bos taurus | D-fructose 6-phosphate + phosphate | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.11 | additional information | - |
fructose 1,6-diphosphate, Mg2+ and Mn2+ protect against thermal inactivation | Bos taurus |
| 3.1.3.11 | 50 | - |
15 min, 50% loss of activity | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.11 | 6.5 | 7.5 | - |
Bos taurus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
