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Literature summary extracted from

  • Rognes, S.E.
    Anion regulation of lupin Asparagine+ synthetase: chloride activation of the glutamine-utilizing reactions (1980), Phytochemistry, 19, 2287-2293.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.4 2-oxoglutarate 20 mM, 12-15% inhibition Lupinus luteus
6.3.5.4 oxaloacetate 20 mM, 40% inhibition Lupinus luteus
6.3.5.4 pyruvate 20 mM, 12-15% inhibition Lupinus luteus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.4 Br- strong activation of Asn synthesis and Gln hydrolysis. The synthetase reactions with NH3 or NH2OH are only slightly stimulated Lupinus luteus
6.3.5.4 Cl- strong activation of Asn synthesis and Gln hydrolysis, competitive activator with respect to Gln and a noncompetitive activator with respect to MgATP2- and Asp. Cl- changes the substrate saturation kinetics of Gln from negatively cooperative to normal hyperbolic and causes a 50fold increase in the affinity for Gln. Inherent glutaminase activity is enhanced 30fold. The synthetase reactions with NH4+ or NH2OH are only slightly stimulated Lupinus luteus
6.3.5.4 CN- stimulates Asn synthesis and Gln hydrolysis Lupinus luteus
6.3.5.4 F- stimulates Asn synthesis and Gln hydrolysis Lupinus luteus
6.3.5.4 I- stimulates Asn synthesis and Gln hydrolysis Lupinus luteus
6.3.5.4 NO3- stimulates Asn synthesis and Gln hydrolysis Lupinus luteus

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.4 Lupinus luteus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.5.4 cotyledon of seedlings Lupinus luteus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.4 ATP + L-Asp + L-Gln
-
Lupinus luteus AMP + diphosphate + Asn + Glu
-
?
6.3.5.4 ATP + L-Asp + NH2OH
-
Lupinus luteus AMP + diphosphate + beta-aspartylhydroxamate
-
?
6.3.5.4 ATP + L-Asp + NH3
-
Lupinus luteus AMP + diphosphate + Asn
-
?
6.3.5.4 additional information enzyme has inherent glutaminase activity Lupinus luteus ?
-
?