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Literature summary extracted from

  • Stehlin, C.; Heacock, D.H.; Liu, H.; Musier-Forsyth, K.
    Chemical modification and site-directed mutagenesis of the single cysteine in motif 3 of class II Escherichia coli prolyl-tRNA synthetase (1997), Biochemistry, 36, 2932-2938.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.15 C443A mutagenesis of C443 to amino acids Ala, Gly and Ser results in significant decreases, 16fold to 225fold in kcat/KmPro, as measured by the ATP-diphosphate exchange assay. The Ala and Gly mutations have relatively small effect, 4fold to 7fold, on the overall aminoacylation reaction, while the activity of the C443 mutant in this same assay is substantially reduced, 80fold Escherichia coli
6.1.1.15 C443G mutagenesis of C443 to amino acids Ala, Gly and Ser results in significant decreases, 16fold to 225fold in kcat/KmPro, as measured by the ATP-diphosphate exchange assay. The Ala and Gly mutations have relatively small effect, 4fold to 7fold, on the overall aminoacylation reaction, while the activity of the C443 mutant in this same assay is substantially reduced, 80fold Escherichia coli
6.1.1.15 C443S mutagenesis of C443 to amino acids Ala, Gly and Ser results in significant decreases, 16fold to 225fold in kcat/KmPro, as measured by the ATP-diphosphate exchange assay. The Ala and Gly mutations have relatively small effect, 4fold to 7fold, on the overall aminoacylation reaction, while the activity of the C443 mutant in this same assay is substantially reduced, 80fold Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.15 iodoacetamide more than 90% protection by 10 mM ATP or 10 mM ATP + 10 mM Pro Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.15 0.3
-
 Pro wild-type Escherichia coli
6.1.1.15 1.7
-
 Pro mutant C443A Escherichia coli
6.1.1.15 2.4
-
 Pro mutant C443G Escherichia coli
6.1.1.15 3.1
-
 Pro mutant C443S Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.15 Escherichia coli
-
 wild-type enzyme and mutant enzymes C443A, C443G, and C443S
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro
-
 Escherichia coli AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.15 3.7
-
 ATP mutant C443S, ATP-diphosphate exchange Escherichia coli
6.1.1.15 15
-
 ATP mutant C443G, ATP-diphosphate exchange Escherichia coli
6.1.1.15 28
-
 ATP mutant C443A, ATP-diphosphate exchange Escherichia coli
6.1.1.15 81
-
 ATP wild-type enzyme, ATP-diphosphate exchange Escherichia coli