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Literature summary extracted from

  • Yu, C.K.; Dietrich, L.S.
    Purification and properties of yeast nicotinamide adenine dinucleotide synthetase (1972), J. Biol. Chem., 247, 4794-4802.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.5.1 repeated freezing and thawing accelerates inactivation Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.1 6-diazo-5-oxo-L-norleucine
-
 Saccharomyces cerevisiae
6.3.5.1 azaserine progressively inhibits glutamine-dependent activity. With NH4+ as amide donor there is an initial 20% inhibition, then the NH4+-dependent activity remains constant Saccharomyces cerevisiae
6.3.5.1 diisopropyl fluorophosphate
-
 Saccharomyces cerevisiae
6.3.5.1 Mn2+ can partially replace Mg2+ at 1 mM. At 5 mM inhibition of NAD+ formation Saccharomyces cerevisiae
6.3.5.1 p-chloromercuribenzoate glutamine-dependent and NH4+-dependent activity Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.1 0.17
-
 ATP pH 7.6 Saccharomyces cerevisiae
6.3.5.1 0.19
-
 deamido-NAD+ pH 7.6 Saccharomyces cerevisiae
6.3.5.1 5
-
 Gln pH 7.6 Saccharomyces cerevisiae
6.3.5.1 6.4
-
 NH4+ pH 8.6 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.1 Co2+ can partially replace Mg2+, at 5 mM 30% of the activity relative to Mg2+ Saccharomyces cerevisiae
6.3.5.1 K+ required Saccharomyces cerevisiae
6.3.5.1 Mg2+ required Saccharomyces cerevisiae
6.3.5.1 Mn2+ can partially replace Mg2+ at 1 mM. At 5 mM inhibition of NAD+ formation Saccharomyces cerevisiae
6.3.5.1 NH4+ as effective as K+ in stimulating activity Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.1 630000
-
 high speed equilibrium ultracentrifugation Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.1 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.1
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
6.3.5.1 4°C, 0.2 mg per ml, 10 d, 60% loss of activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.1 ATP + deamido-NAD+ + Gln + hydroxylamine
-
 Saccharomyces cerevisiae AMP + diphosphate + hydroxamate analog + ?
-
 ?
6.3.5.1 ATP + deamido-NAD+ + L-Gln
-
 Saccharomyces cerevisiae AMP + diphosphate + NAD+ + L-Glu
-
 ?
6.3.5.1 ATP + deamido-NAD+ + NH4+
-
 Saccharomyces cerevisiae AMP + diphosphate + NAD+
-
 ?
6.3.5.1 Gln + hydroxylamine
-
 Saccharomyces cerevisiae AMP + diphosphate + hydroxamate analog + ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.5.1 additional information
-
 additional information turnover number: 1260, NAD+ production Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.1 6.2 8.4 broad peak of activity with a maximum around 7.6, with Gln as the amide donor Saccharomyces cerevisiae
6.3.5.1 8.4 8.8 with NH4+ as amide donor Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.5.1 7.8 9.3 7.8: about 40% of maximal activity, 9.3: about 50% of maximal activity, with NH4+ as amide donor Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.5.1 6 10 overnight, no loss of activity Saccharomyces cerevisiae