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Literature summary extracted from

  • Roon, R.J.; Levenberg, B.
    Urea amidolyase. I. Properties of the enzyme from Candida utilis (1972), J. Biol. Chem., 247, 4107-4113.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.6 ADP
-
 Cyberlindnera jadinii
6.3.4.6 Avidin
-
 Chlamydomonas reinhardtii
6.3.4.6 Avidin
-
 Cyberlindnera jadinii
6.3.4.6 Avidin
-
 Saccharomyces cerevisiae
6.3.4.6 BaCl2
-
 Cyberlindnera jadinii
6.3.4.6 CaCl2
-
 Cyberlindnera jadinii
6.3.4.6 CoCl2
-
 Cyberlindnera jadinii
6.3.4.6 F-
-
 Cyberlindnera jadinii
6.3.4.6 HgCl2
-
 Cyberlindnera jadinii
6.3.4.6 iodoacetate
-
 Cyberlindnera jadinii
6.3.4.6 PCMB
-
 Cyberlindnera jadinii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.6 0.1
-
 Urea
-
 Cyberlindnera jadinii
6.3.4.6 0.1
-
 Urea
-
 Saccharomyces cerevisiae
6.3.4.6 0.25
-
 ATP
-
 Cyberlindnera jadinii
6.3.4.6 0.5
-
 HCO3-
-
 Cyberlindnera jadinii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.6 Cs+ monovalent cation required, K+, Rb+, Cs+, or NH4+ Cyberlindnera jadinii
6.3.4.6 K+ monovalent cation required, K+, Rb+, Cs+ or NH4+ Cyberlindnera jadinii
6.3.4.6 Mg2+ divalent cation required, Mg2+ or Mn2+, maximal activity at 8 mM Cyberlindnera jadinii
6.3.4.6 Mn2+ divalent cation required, Mg2+ or Mn2+, maximal activity at 2 mM, much less effective than Mg2+ at the optimal level Cyberlindnera jadinii
6.3.4.6 NH4+ monovalent cation required, K+, Rb+, Cs+, or NH4+ Cyberlindnera jadinii
6.3.4.6 Rb+ monovalent cation required, K+, Rb+, Cs+, or NH4+ Cyberlindnera jadinii

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.6 Auxenochlorella pyrenoidosa
-
 grown on urea as sole nitogen source
-
6.3.4.6 Chlamydomonas reinhardtii
-
 grown on urea as sole nitrogen source
-
6.3.4.6 Cyberlindnera jadinii
-
-
-
6.3.4.6 Cyberlindnera jadinii
-
 inducible enzyme
-
6.3.4.6 Debaryomyces subglobosus
-
 grown on urea as sole nitrogen source
-
6.3.4.6 Pseudochlorella pringsheimii
-
 grown on urea as sole nitogen source
-
6.3.4.6 Saccharomyces cerevisiae
-
 grown on urea as sole nitrogen source
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.6 ATP + acetamide + HCO3-
-
 Cyberlindnera jadinii ?
-
 ?
6.3.4.6 ATP + acetamide + HCO3-
-
 Saccharomyces cerevisiae ?
-
 ?
6.3.4.6 ATP + cyanamide + HCO3-
-
 Cyberlindnera jadinii ?
-
 ?
6.3.4.6 ATP + formamide + HCO3-
-
 Cyberlindnera jadinii ?
-
 ?
6.3.4.6 ATP + formamide + HCO3-
-
 Saccharomyces cerevisiae ?
-
 ?
6.3.4.6 ATP + hydroxyurea + HCO3-
-
 Cyberlindnera jadinii ?
-
 ?
6.3.4.6 ATP + hydroxyurea + HCO3-
-
 Saccharomyces cerevisiae ?
-
 ?
6.3.4.6 ATP + imidodicarbonic acid diamide + HCO3- iminodicarbonic acid diamide, i.e. biuret Cyberlindnera jadinii ?
-
 ?
6.3.4.6 ATP + N-methylurea + HCO3-
-
 Cyberlindnera jadinii ?
-
 ?
6.3.4.6 ATP + N-methylurea + HCO3-
-
 Saccharomyces cerevisiae ?
-
 ?
6.3.4.6 ATP + propionamide + HCO3-
-
 Cyberlindnera jadinii ?
-
 ?
6.3.4.6 ATP + urea + HCO3-
-
 Chlamydomonas reinhardtii ADP + phosphate + allophanate
-
 ?
6.3.4.6 ATP + urea + HCO3-
-
 Cyberlindnera jadinii ADP + phosphate + allophanate
-
 ?
6.3.4.6 ATP + urea + HCO3-
-
 Saccharomyces cerevisiae ADP + phosphate + allophanate
-
 ?
6.3.4.6 ATP + urea + HCO3-
-
 Pseudochlorella pringsheimii ADP + phosphate + allophanate
-
 ?
6.3.4.6 ATP + urea + HCO3-
-
 Auxenochlorella pyrenoidosa ADP + phosphate + allophanate
-
 ?
6.3.4.6 ATP + urea + HCO3-
-
 Debaryomyces subglobosus ADP + phosphate + allophanate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.4.6 30
-
 stable for short periods of time Cyberlindnera jadinii
6.3.4.6 45
-
 10 min, 50% loss of activity Cyberlindnera jadinii
6.3.4.6 55
-
 10 min, complete denaturation Cyberlindnera jadinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.6 7.8 7.9
-
 Cyberlindnera jadinii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.4.6 7.3 8.7 about 50% of maximal activity at pH 7.3 and 8.7 Cyberlindnera jadinii