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Literature summary extracted from

  • Spector, T.; Jones, T.E.; Elion, G.B.
    Specificity of adenylosuccinate synthetase and adenylosuccinate lyase from Leishmania donovani. Selective amination of an antiprotozoal agent (1979), J. Biol. Chem., 254, 8422-8426.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.3.2.2 unstable to quick freezing in dry ice/acetone Leishmania donovani

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.2.2 2'-deoxy-AMP
-
 Leishmania donovani
4.3.2.2 4-aminopyrazolo[3,4-d]pyrimidine ribose 5'-phosphate
-
 Leishmania donovani
4.3.2.2 8-Aza-AMP
-
 Leishmania donovani
4.3.2.2 AMP competitive Leishmania donovani
4.3.2.2 arabinosyl-AMP
-
 Leishmania donovani
4.3.2.2 EDTA above 15 mM Leishmania donovani
4.3.2.2 fumarate noncompetitive Leishmania donovani
4.3.2.2 MgCl2
-
 Leishmania donovani
4.3.2.2 phosphate competitive Leishmania donovani
6.3.4.4 (NH4)2SO4 25 mM Leishmania donovani
6.3.4.4 2'-GMP
-
 Leishmania donovani
6.3.4.4 3'-GMP
-
 Leishmania donovani
6.3.4.4 6-Thio-GMP
-
 Leishmania donovani
6.3.4.4 8-Aza-7-deaza-GMP
-
 Leishmania donovani
6.3.4.4 8-aza-GMP
-
 Leishmania donovani
6.3.4.4 acyclo-GMP
-
 Leishmania donovani
6.3.4.4 arabinosyl-GMP
-
 Leishmania donovani
6.3.4.4 cyclic GMP
-
 Leishmania donovani
6.3.4.4 dGMP 2'-dGMP Leishmania donovani
6.3.4.4 GMP and analogs Leishmania donovani
6.3.4.4 KCl 75 mM Leishmania donovani
6.3.4.4 phosphoenolpyruvate
-
 Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.2 0.0008
-
 2'-deoxy-succino-AMP
-
 Leishmania donovani
4.3.2.2 0.0027
-
 arabinosyl-succino-AMP
-
 Leishmania donovani
4.3.2.2 0.0033
-
 N6-(1,2-dicarboxyethyl)AMP
-
 Leishmania donovani
4.3.2.2 0.004
-
 AMP
-
 Leishmania donovani
4.3.2.2 0.0095
-
 8-aza-succino-AMP
-
 Leishmania donovani
4.3.2.2 0.011
-
 succino-4-aminopyrazolo[3,4-d]pyrimidine ribose 5'-phosphate
-
 Leishmania donovani
6.3.4.4 0.012
-
 GTP IMP Leishmania donovani
6.3.4.4 0.05
-
 8-azaIMP
-
 Leishmania donovani
6.3.4.4 0.26
-
 arabinosylIMP
-
 Leishmania donovani
6.3.4.4 0.34
-
 allopurinol ribonucleotide
-
 Leishmania donovani
6.3.4.4 0.48
-
 2'-dIMP
-
 Leishmania donovani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.2.2 300000
-
 gel filtration Leishmania donovani
6.3.4.4 250000
-
 gel filtration Leishmania donovani

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.2 Leishmania donovani
-
-
-
6.3.4.4 Leishmania donovani
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.2
-
 Leishmania donovani

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.2.2 promastigote
-
 Leishmania donovani
-

Storage Stability

EC Number Storage Stability Organism
4.3.2.2 -70°C, unstable to storage Leishmania donovani
4.3.2.2 2°C or -20°C, stable for two months Leishmania donovani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.2 2'-deoxy-succino-AMP
-
 Leishmania donovani 2'-deoxy-AMP + fumarate
-
 ?
4.3.2.2 8-aza-succino-AMP
-
 Leishmania donovani 8-aza-AMP + fumarate
-
 ?
4.3.2.2 arabinosyl-succino-AMP
-
 Leishmania donovani arabinosyl-AMP + ?
-
 ?
4.3.2.2 fumarate + AMP
-
 Leishmania donovani N6-(1,2-dicarboxyethyl)AMP
-
 r
4.3.2.2 N6-(1,2-dicarboxyethyl)AMP i.e. adenylosuccinate Leishmania donovani fumarate + AMP
-
 r
4.3.2.2 succino-4-aminopyrazolo[3,4-d]pyrimidine ribose 5'-phosphate
-
 Leishmania donovani 4-aminopyrazolo[3,4-d]pyrimidine ribose 5'-phosphate + ?
-
 ?
6.3.4.4 GTP + 2'-dIMP + L-Asp
-
 Leishmania donovani GDP + phosphate + 2'-deoxysuccinoAMP
-
 ?
6.3.4.4 GTP + 4-hydroxypyrazolo[3,4-d]pyrimidine ribonucleotide + L-Asp i.e. allopurinol ribonucleotide Leishmania donovani GDP + phosphate + 4-aminopyrazolo[3,4-d]pyrimidine ribonucleotide
-
 ?
6.3.4.4 GTP + 8-azaIMP + L-Asp
-
 Leishmania donovani GDP + phosphate + 8-azasuccinoAMP
-
 ?
6.3.4.4 GTP + beta-D-arabinosylIMP + L-Asp
-
 Leishmania donovani GDP + phosphate + arabinosylsuccinoAMP
-
 ?
6.3.4.4 GTP + IMP + L-Asp
-
 Leishmania donovani GDP + phosphate + adenylosuccinate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.2.2 7.7 8.7 Tris buffer Leishmania donovani
6.3.4.4 6.3 6.8
-
 Leishmania donovani