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Literature summary extracted from

  • Frenzel, T.; Zenk, M.H.
    Purification and characterization of three isoforms of S-adenosyl-L-methionine: (R,S)-tetrahydrobenzylisoquinoline-N-methyltransferase from Berberis koetineana cell cultures (1990), Phytochemistry, 29, 3491-3497.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.1.1.115 synthesis the enzyme immobilized on CH-Sepharose or CPG-10 glass beads is a useful tool for the preparative synthesis of isotopically labelled N-methylated benzylisoquinoline alkaloids Berberis koetineana

General Stability

EC Number General Stability Organism
2.1.1.115 -20°C, in presence of 20% glycerol, half-life of immobilized enzyme: 40 days Berberis koetineana
2.1.1.115 6°C, in presence of 20% glycerol, half-life of immobilized enzyme: 20 days Berberis koetineana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.115 0.0015
-
 (S)-6-O-methylnorlaudanosoline isoenzyme NMT-III Berberis koetineana
2.1.1.115 0.003
-
 (S)-6-O-methylnorlaudanosoline isoenzyme NMT-II Berberis koetineana
2.1.1.115 0.004
-
 (R)-6-O-methylnorlaudanosoline isoenzyme NMT-II Berberis koetineana
2.1.1.115 0.005
-
 (S)-norreticuline isoenzyme NMT-III Berberis koetineana
2.1.1.115 0.005
-
 (S)-norreticuline isoenzyme NMT-I Berberis koetineana
2.1.1.115 0.009
-
 (S)-norreticuline isoenzyme NMT-II Berberis koetineana
2.1.1.115 0.012
-
 (R)-norreticuline isoenzyme NMT-III Berberis koetineana
2.1.1.115 0.012
-
 (R)-norreticuline isoenzyme NMT-I Berberis koetineana
2.1.1.115 0.015
-
 (R)-norreticuline isoenzyme NMT-II Berberis koetineana
2.1.1.115 0.017
-
 (S)-norreticuline isoenzyme NMT-I Berberis koetineana
2.1.1.115 0.018
-
 (R)-norreticuline isoenzyme NMT-I Berberis koetineana
2.1.1.115 0.05
-
 (R)-6-O-methylnorlaudanosoline isoenzyme NMT-III Berberis koetineana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.115 60000
-
 isoenzyme NMT-I and NMT-III, gel filtration Berberis koetineana
2.1.1.115 68000
-
 gel filtration Berberis vulgaris
2.1.1.115 78000
-
 isoenzyme NMT-II, gel filtration Berberis koetineana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.115 S-Adenosyl-L-methionine + coclaurine Berberis koetineana
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.115 Argemone platyceras
-
-
-
2.1.1.115 Berberis angulosa
-
-
-
2.1.1.115 Berberis beaniana
-
-
-
2.1.1.115 Berberis bretschneideri
-
-
-
2.1.1.115 Berberis carminea
-
-
-
2.1.1.115 Berberis crataegina
-
-
-
2.1.1.115 Berberis dictyophylla
-
-
-
2.1.1.115 Berberis gagnepainii
-
-
-
2.1.1.115 Berberis henryana
-
-
-
2.1.1.115 Berberis julianae
-
-
-
2.1.1.115 Berberis koetineana
-
 3 isoenzymes: NMT-I, NMT-II, NMT-III
-
2.1.1.115 Berberis laxifolia
-
-
-
2.1.1.115 Berberis nervosa
-
-
-
2.1.1.115 Berberis stenophylla
-
-
-
2.1.1.115 Berberis stolonifera
-
 V 29
-
2.1.1.115 Berberis stolonifera V 29
-
 V 29
-
2.1.1.115 Berberis taliensis
-
-
-
2.1.1.115 Berberis thunbergii
-
 Atropurpurea
-
2.1.1.115 Berberis turcomanica
-
-
-
2.1.1.115 Berberis vulgaris
-
-
-
2.1.1.115 Berberis wilsoniae
-
-
-
2.1.1.115 Bocconia cordata
-
-
-
2.1.1.115 Coptis japonica
-
-
-
2.1.1.115 Corydalis vaginans
-
-
-
2.1.1.115 Eschscholzia californica
-
-
-
2.1.1.115 Glaucium corniculatum
-
-
-
2.1.1.115 Glaucium elegans
-
-
-
2.1.1.115 Glaucium flavum
-
-
-
2.1.1.115 Hyalosepalum caffrum
-
-
-
2.1.1.115 Podophyllum peltatum
-
-
-
2.1.1.115 Sinopodophyllum hexandrum
-
-
-
2.1.1.115 Stephania delavayi
-
-
-
2.1.1.115 Thalictrum dipterocarpum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.115
-
 Berberis koetineana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.115 cell culture
-
 Berberis koetineana
-

Storage Stability

EC Number Storage Stability Organism
2.1.1.115 -20°C, in presence of 20% glycerol, half-life of immobilized enzyme: 40 days Berberis koetineana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.115 S-Adenosyl-L-methionine + (R)-6-O-methylnorlaudanosoline
-
 Berberis koetineana ?
-
 ?
2.1.1.115 S-Adenosyl-L-methionine + (R)-norcoclaurine
-
 Berberis koetineana ?
-
 ?
2.1.1.115 S-Adenosyl-L-methionine + (R)-norprotosinomenine isoenzyme NMT-I: 6% of maximal activity with (R)-tetrahydropapaverine Berberis koetineana ?
-
 ?
2.1.1.115 S-Adenosyl-L-methionine + (R,S)-4'-O-methylnorlaudanosoline isoenzyme NMT-I: 3% of maximal activity with (R)-tetrahydropapaverine Berberis koetineana ?
-
 ?
2.1.1.115 S-Adenosyl-L-methionine + (R,S)-7-O-methylnorlaudanosoline isoenzyme NMT-I: 12% of maximal activity with (R)-tetrahydropapaverine Berberis koetineana ?
-
 ?
2.1.1.115 S-Adenosyl-L-methionine + (R,S)-isococlaurine
-
 Berberis koetineana ?
-
 ?
2.1.1.115 S-Adenosyl-L-methionine + (R,S)-norarmepavine
-
 Berberis koetineana ?
-
 ?
2.1.1.115 S-Adenosyl-L-methionine + (R,S)-norcodamine
-
 Berberis koetineana ?
-
 ?
2.1.1.115 S-Adenosyl-L-methionine + (R,S)-nororientaline
-
 Berberis koetineana ?
-
 ?
2.1.1.115 S-Adenosyl-L-methionine + (RS)-1-benzyl-1,2,3,4-tetrahydroisoquinoline
-
 Berberis koetineana ?
-
 ?
2.1.1.115 S-Adenosyl-L-methionine + (S)-6-O-methylnorlaudanosoline
-
 Berberis koetineana ?
-
 ?
2.1.1.115 S-Adenosyl-L-methionine + (S)-norcoclaurine
-
 Berberis koetineana ?
-
 ?
2.1.1.115 S-Adenosyl-L-methionine + coclaurine
-
 Berberis koetineana ?
-
 ?
2.1.1.115 S-Adenosylmethionine + (R)-coclaurine isoenzyme NMT-II and NMT-III show maximal activity with (R)-coclaurine Berberis koetineana S-Adenosyl-L-homocysteine + N-methyl-(RS)-1-benzyl-1,2,3,4-tetrahydroisoquinoline
-
 ?
2.1.1.115 S-Adenosylmethionine + (R)-norreticuline
-
 Berberis koetineana ?
-
 ?
2.1.1.115 S-Adenosylmethionine + (R)-tetrahydropapaverine isoenzyme NMT-I shows maximal activity with (R)-tetrahydropapaverine Berberis koetineana ?
-
 ?
2.1.1.115 S-Adenosylmethionine + (S)-coclaurine
-
 Berberis koetineana S-Adenosylhomocysteine + N-methylcoclaurine
-
 ?
2.1.1.115 S-Adenosylmethionine + (S)-norreticuline
-
 Berberis koetineana S-Adenosylhomocysteine + (S)-scoulerine
-
 ?
2.1.1.115 S-Adenosylmethionine + (S)-tetrahydropapaverine
-
 Berberis koetineana ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.115 35 40 isoenzyme NMT-I, NMT-II and NMT-III Berberis koetineana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.115 6
-
 in presence of 20% glycerol, half-life of immobilized enzyme: 20 days Berberis koetineana
2.1.1.115 20
-
 in presence of 20% glycerol, half-life of immobilized enzyme: 7 days Berberis koetineana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.115 6.8
-
 isoenzyme NMT-III Berberis koetineana
2.1.1.115 7.4
-
 isoenzyme NMT-I and NMT-II Berberis koetineana