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Literature summary extracted from

  • Wong, K.K.; Murray, B.W.; Lewisch, S.A.; Baxter, M.K.; Ridky, T.W.; Ulissi-DeMario, L.; Kozarich, J.W.
    Molecular properties of pyruvate formate-lyase activating enzyme (1993), Biochemistry, 32, 14102-14110.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.97.1.4 overexpression in Escherichia coli Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.97.1.4 Cd2+
-
 Escherichia coli
1.97.1.4 Cu2+
-
 Escherichia coli
1.97.1.4 Zn2+
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.97.1.4 0.0012
-
 [pyruvate formate-lyase]-glycine
-
 Escherichia coli
1.97.1.4 0.0028
-
 S-adenosyl-L-methionine
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.97.1.4 inclusion body insoluble Escherichia coli 16234
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.97.1.4 Cobalt Co(II) and Cu(II) can be reconstituted into the protein with similar stoichiometry Escherichia coli
1.97.1.4 copper Co(II) and Cu(II) can be reconstituted into the protein with similar stoichiometry Escherichia coli
1.97.1.4 Iron binds one Fe(II) per protein monomer. Co(II) and Cu(II) can be reconstituted into the protein with similar stoichiometry Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.97.1.4 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.97.1.4
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.97.1.4 S-adenosyl-L-methionine + dihydroflavodoxin + formate acetyltransferase-glycine
-
 Escherichia coli 5'-deoxyadenosine + methionine + flavodoxin + formate acetyltransferase-glycine-2-yl-radical
-
 ?
1.97.1.4 S-adenosyl-L-methionine + dihydroflavodoxin + [pyruvate formate-lyase]-glycine
-
 Escherichia coli methionine + 5'-deoxyadenosine + [pyruvate formate-lyase]-glycine radical + flavodoxin
-
 r