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Literature summary extracted from
- Dehydroquinate synthase from Escherichia coli, and its substrate 3-deoxy-D-arabino-heptulosonic acid 7-phosphate (1987), Methods Enzymol., 142, 306-314.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.3.4 | overexpression in Escherichia coli | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.3.4 | EGTA | - |
Escherichia coli |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.3.4 | 0.033 | 0.055 | 3-deoxy-D-arabino-heptulosonic acid 7-phosphate | - |
Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.4 | 38880 | - |
HPLC | Escherichia coli |
| 4.2.3.4 | 38880 | - |
denaturing gel electrophoresis | Escherichia coli |
| 4.2.3.4 | 44000 | - |
HPLC | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.4 | 3-deoxy-D-arabino-heptulosonic acid 7-phosphate | Escherichia coli | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.3.4 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.3.4 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.4 | 44 | - |
- |
Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.2.3.4 | -70°C, more than 1 year | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.4 | 3-deoxy-D-arabino-heptulosonic acid 7-phosphate | - |
Escherichia coli | 3-dehydroquinate + phosphate | - |
ir | |
| 4.2.3.4 | 3-deoxy-D-arabino-heptulosonic acid 7-phosphate | - |
Escherichia coli | ? | - |
? | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.3.4 | NAD+ | 1 mol of NAD+ per mol of protein | Escherichia coli | |
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